Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PN942_RS02725 Genome accession   NZ_CP116807
Coordinates   546956..547852 (-) Length   298 a.a.
NCBI ID   WP_017000235.1    Uniprot ID   A0AAP1RT50
Organism   Mammaliicoccus lentus strain PVZ.22     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 541956..552852
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PN942_RS02690 (PN942_02685) - 542404..542922 (-) 519 WP_017000243.1 shikimate kinase -
  PN942_RS02695 (PN942_02690) - 543188..544360 (-) 1173 WP_000195429.1 IS256-like element IS256 family transposase -
  PN942_RS02700 (PN942_02695) comGF 544578..544925 (-) 348 WP_282461086.1 competence type IV pilus minor pilin ComGF -
  PN942_RS02705 (PN942_02700) comGE 544900..545172 (-) 273 WP_228082628.1 competence type IV pilus minor pilin ComGE -
  PN942_RS02710 (PN942_02705) comGD 545159..545587 (-) 429 WP_017000239.1 competence type IV pilus minor pilin ComGD -
  PN942_RS02715 (PN942_02710) comGC 545587..545904 (-) 318 WP_070045385.1 competence type IV pilus major pilin ComGC Machinery gene
  PN942_RS02720 (PN942_02715) comGB 545931..546956 (-) 1026 WP_282461087.1 competence type IV pilus assembly protein ComGB -
  PN942_RS02725 (PN942_02720) comGA 546956..547852 (-) 897 WP_017000235.1 competence type IV pilus ATPase ComGA Machinery gene
  PN942_RS02730 (PN942_02725) - 548005..548253 (+) 249 WP_017000234.1 DUF2626 family protein -
  PN942_RS02735 (PN942_02730) - 548318..548950 (-) 633 WP_017000233.1 MBL fold metallo-hydrolase -
  PN942_RS02740 (PN942_02735) - 549048..549287 (+) 240 WP_017000232.1 DUF2759 domain-containing protein -
  PN942_RS02745 (PN942_02740) - 549316..549630 (-) 315 WP_282461088.1 MTH1187 family thiamine-binding protein -
  PN942_RS02750 (PN942_02745) - 549630..550601 (-) 972 WP_017000230.1 ROK family glucokinase -
  PN942_RS02755 (PN942_02750) - 550598..550789 (-) 192 WP_017000229.1 YqgQ family protein -
  PN942_RS02760 (PN942_02755) - 550792..552240 (-) 1449 WP_017000228.1 rhomboid family intramembrane serine protease -
  PN942_RS02765 (PN942_02760) - 552241..552795 (-) 555 WP_064204534.1 5-formyltetrahydrofolate cyclo-ligase -

Sequence

Protein


Download         Length: 298 a.a.        Molecular weight: 34435.59 Da        Isoelectric Point: 5.8696

>NTDB_id=780351 PN942_RS02725 WP_017000235.1 546956..547852(-) (comGA) [Mammaliicoccus lentus strain PVZ.22]
METLFKTIIKDAIILKATDIHFIPVKEDRVKVQLRISGENTIYDCDINNALYNRLLIYMKFIAHLDVSERNKAQSGQYIF
DDIYKYYLRISTLPLSLGIESCSIRLTPKYFEEYIDHNIDDNYKIIKEEMFNSEGLFLFTGPTGSGKSTIMYQFLYDLQQ
IKDKHIITIEDPVEQVLDGVVQVSVNEKANINYHNSFKAILRCDPDVVMIGEIRDEETAKQVIQASLSGHLILSTMHAKD
NLGAIYRLLELGISKEQIKQGIACIANQRLLNDQLGNRYRQMTYIFSHEINLYLENKS

Nucleotide


Download         Length: 897 bp        

>NTDB_id=780351 PN942_RS02725 WP_017000235.1 546956..547852(-) (comGA) [Mammaliicoccus lentus strain PVZ.22]
ATGGAAACTTTATTTAAGACAATTATCAAAGATGCTATTATTTTGAAAGCAACTGACATTCATTTTATTCCAGTGAAAGA
GGATCGGGTCAAAGTTCAGTTGAGGATAAGTGGTGAGAATACGATTTATGATTGTGATATTAATAACGCTTTATATAATC
GTTTATTAATTTATATGAAGTTTATTGCTCATCTAGATGTAAGTGAAAGGAATAAAGCTCAAAGTGGTCAATATATATTT
GATGATATATATAAATATTATTTAAGAATATCCACTTTACCTTTAAGTCTAGGAATTGAGTCTTGCTCAATTAGGTTAAC
GCCTAAATATTTCGAAGAATATATTGACCATAATATTGATGACAATTATAAAATAATTAAAGAAGAAATGTTTAATTCCG
AAGGCTTATTTTTGTTCACAGGACCAACCGGTTCGGGTAAATCGACGATTATGTACCAATTTTTATATGACTTACAACAA
ATTAAAGATAAACATATTATTACGATTGAAGACCCAGTTGAACAAGTTTTAGATGGTGTAGTACAAGTTTCTGTAAATGA
AAAAGCAAACATAAATTATCATAATTCTTTTAAAGCCATTTTAAGATGTGATCCAGATGTTGTCATGATAGGAGAAATTA
GAGATGAAGAAACAGCCAAGCAAGTTATACAAGCAAGTTTAAGTGGTCATTTAATATTATCTACAATGCATGCGAAAGAT
AATTTAGGGGCTATTTATAGACTTTTAGAACTTGGAATATCTAAAGAGCAAATAAAACAAGGTATCGCATGTATAGCCAA
TCAAAGACTTTTAAATGACCAATTAGGAAATAGATATAGACAAATGACTTATATATTTAGTCATGAAATTAATCTATATT
TAGAAAATAAATCATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-272)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Staphylococcus aureus MW2

50.993

100

0.517
  comGA Staphylococcus aureus N315

50.993

100

0.517
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

39.068

93.624

0.366