Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   PNF29_RS21230 Genome accession   NZ_CP116773
Coordinates   4002860..4004236 (-) Length   458 a.a.
NCBI ID   WP_015252992.1    Uniprot ID   A0A0C3LIP7
Organism   Bacillus subtilis strain SRCM125727     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 3997860..4009236
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF29_RS21210 (PNF29_21210) ispF 3999376..3999852 (-) 477 WP_003225745.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  PNF29_RS21215 (PNF29_21215) ispD 3999845..4000543 (-) 699 WP_003235019.1 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase -
  PNF29_RS21220 (PNF29_21220) yacL 4000558..4001658 (-) 1101 WP_017695823.1 PIN/TRAM domain-containing protein -
  PNF29_RS21225 (PNF29_21225) disA 4001774..4002856 (-) 1083 WP_003225736.1 DNA integrity scanning diadenylate cyclase DisA -
  PNF29_RS21230 (PNF29_21230) radA 4002860..4004236 (-) 1377 WP_015252992.1 DNA repair protein RadA Machinery gene
  PNF29_RS21235 (PNF29_21235) clpC 4004328..4006760 (-) 2433 WP_014475592.1 ATP-dependent protease ATP-binding subunit ClpC Regulator
  PNF29_RS21240 (PNF29_21240) mcsB 4006757..4007848 (-) 1092 WP_003235007.1 protein arginine kinase -
  PNF29_RS21245 (PNF29_21245) mcsA 4007848..4008405 (-) 558 WP_017695822.1 protein-arginine kinase activator protein McsA -
  PNF29_RS21250 (PNF29_21250) ctsR 4008419..4008883 (-) 465 WP_003225724.1 transcriptional regulator CtsR -

Sequence

Protein


Download         Length: 458 a.a.        Molecular weight: 49438.94 Da        Isoelectric Point: 8.2399

>NTDB_id=780146 PNF29_RS21230 WP_015252992.1 4002860..4004236(-) (radA) [Bacillus subtilis strain SRCM125727]
MAKTKSKFICQSCGYESPKWMGKCPGCGAWNTMVEEMIKKAPANRRAAFSHSVQTVQKPSPITSIETSEEPRVKTQLGEF
NRVLGGGVVKGSLVLIGGDPGIGKSTLLLQVSAQLSGSSNSVLYISGEESVKQTKLRADRLGINNPSLHVLSETDMEYIS
SAIQEMNPAFVVVDSIQTVYQSDITSAPGSVSQVRECTAELMKIAKTKGIPIFIVGHVTKEGSIAGPRLLEHMVDTVLYF
EGERHHTFRILRAVKNRFGSTNEMGIFEMREEGLTEVLNPSEIFLEERSAGSAGSSITASMEGTRPILVEIQALISPTSF
GNPRRMATGIDHNRVSLLMAVLEKRVGLLLQNQDAYLKVAGGVKLDEPAIDLAIAISIASSFRDTPPNPADCFIGEVGLT
GEVRRVSRIEQRVKEAAKLGFKRMIIPAANLDGWTKPKGIEVIGVANVAEALRTSLGG

Nucleotide


Download         Length: 1377 bp        

>NTDB_id=780146 PNF29_RS21230 WP_015252992.1 4002860..4004236(-) (radA) [Bacillus subtilis strain SRCM125727]
ATGGCTAAAACAAAATCGAAGTTCATCTGCCAATCCTGCGGCTACGAGTCTCCAAAATGGATGGGGAAATGTCCGGGCTG
TGGTGCTTGGAATACAATGGTGGAAGAAATGATTAAAAAAGCACCGGCCAATCGGAGAGCGGCTTTTTCTCATTCTGTTC
AAACTGTACAGAAACCTTCACCTATTACATCAATCGAAACATCAGAAGAACCCCGCGTCAAAACCCAGCTTGGCGAGTTT
AACAGAGTACTCGGCGGAGGTGTCGTTAAAGGCTCCCTCGTTTTAATTGGCGGTGATCCTGGTATCGGAAAGTCAACGCT
ATTACTGCAGGTTTCCGCTCAATTATCAGGCTCATCAAACAGTGTCCTGTATATTTCGGGAGAAGAATCTGTAAAGCAAA
CGAAGCTGCGAGCAGACCGTCTCGGCATTAATAATCCGTCACTGCATGTTTTATCTGAAACCGATATGGAGTATATTTCG
TCTGCTATACAAGAGATGAATCCAGCGTTTGTCGTTGTTGACTCTATTCAAACGGTTTACCAAAGCGATATTACATCGGC
TCCAGGCAGTGTGTCACAGGTCAGAGAATGTACCGCTGAGCTGATGAAAATTGCAAAAACAAAAGGTATTCCGATTTTTA
TCGTAGGGCACGTGACGAAAGAAGGGTCTATTGCAGGTCCGAGACTGTTGGAGCATATGGTTGACACTGTTTTATATTTT
GAAGGAGAACGCCACCATACTTTCCGTATTTTGCGGGCTGTAAAAAACCGTTTCGGATCCACAAACGAAATGGGCATTTT
CGAAATGCGGGAAGAGGGGCTCACTGAGGTTTTGAATCCTTCGGAAATTTTCTTAGAAGAACGCTCTGCTGGTTCCGCAG
GCTCGAGTATCACTGCCTCTATGGAGGGCACTAGACCGATTCTCGTTGAAATTCAGGCGCTCATCTCGCCAACAAGCTTT
GGCAACCCAAGGCGTATGGCAACGGGAATAGACCATAACAGAGTTTCTCTGTTAATGGCTGTGTTAGAAAAAAGAGTTGG
ACTGCTGCTGCAAAATCAGGATGCTTATTTGAAAGTGGCTGGCGGCGTTAAGCTTGATGAACCGGCAATTGATCTTGCCA
TTGCGATTAGTATCGCATCAAGCTTTAGAGACACACCTCCAAATCCTGCAGATTGCTTTATTGGAGAAGTGGGATTAACC
GGAGAAGTCCGGCGGGTTTCAAGAATTGAACAGCGTGTGAAAGAAGCGGCAAAGCTTGGTTTTAAACGCATGATCATACC
CGCGGCAAATCTGGATGGATGGACAAAACCAAAAGGGATTGAGGTTATCGGAGTAGCAAATGTTGCAGAAGCGCTTCGTA
CTTCATTAGGAGGATAA

Domains

Predicted by InterproScan.

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(8-35)

AAA domain (AAA_25)

(72-222)

Subunit ChlI of Mg-chelatase (ChlI)

(365-430)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0C3LIP7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Bacillus subtilis subsp. subtilis str. 168

99.563

100

0.996
  radA Streptococcus mitis NCTC 12261

63.355

98.908

0.627
  radA Streptococcus pneumoniae Rx1

63.355

98.908

0.627
  radA Streptococcus pneumoniae D39

63.355

98.908

0.627
  radA Streptococcus pneumoniae R6

63.355

98.908

0.627
  radA Streptococcus pneumoniae TIGR4

63.355

98.908

0.627
  radA Streptococcus mitis SK321

63.135

98.908

0.624