Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   PNF29_RS03015 Genome accession   NZ_CP116773
Coordinates   578042..579433 (+) Length   463 a.a.
NCBI ID   WP_003243962.1    Uniprot ID   A0AAE2SL32
Organism   Bacillus subtilis strain SRCM125727     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 573042..584433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PNF29_RS02995 (PNF29_02995) yvyE 574194..574847 (-) 654 WP_003227979.1 YigZ family protein -
  PNF29_RS03000 (PNF29_03000) degS 575064..576221 (+) 1158 WP_272515926.1 two-component sensor histidine kinase DegS Regulator
  PNF29_RS03005 (PNF29_03005) degU 576304..576993 (+) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  PNF29_RS03010 (PNF29_03010) fakBA 577091..577936 (+) 846 WP_272515927.1 DegV family protein -
  PNF29_RS03015 (PNF29_03015) comFA 578042..579433 (+) 1392 WP_003243962.1 ATP-dependent helicase ComFA Machinery gene
  PNF29_RS03020 (PNF29_03020) comFB 579493..579789 (+) 297 WP_003227989.1 late competence protein ComFB -
  PNF29_RS03025 (PNF29_03025) comFC 579753..580475 (+) 723 WP_129137967.1 comF operon protein ComFC Machinery gene
  PNF29_RS03030 (PNF29_03030) yvyF 580549..580968 (+) 420 WP_041351272.1 TIGR03826 family flagellar region protein -
  PNF29_RS03035 (PNF29_03035) flgM 581049..581315 (+) 267 WP_014478128.1 flagellar biosynthesis anti-sigma factor FlgM -
  PNF29_RS03040 (PNF29_03040) flgN 581331..581813 (+) 483 WP_041351273.1 flagellar protein FlgN -
  PNF29_RS03045 (PNF29_03045) flgK 581832..583355 (+) 1524 WP_041351274.1 flagellar hook-associated protein FlgK -
  PNF29_RS03050 (PNF29_03050) flgL 583366..584262 (+) 897 WP_015714797.1 flagellar hook-associated protein FlgL -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52565.63 Da        Isoelectric Point: 10.0082

>NTDB_id=780084 PNF29_RS03015 WP_003243962.1 578042..579433(+) (comFA) [Bacillus subtilis strain SRCM125727]
MNVPVEKNSSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISINKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHVKEGRPVFLFVPSVSILEKAAACFKGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESSIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=780084 PNF29_RS03015 WP_003243962.1 578042..579433(+) (comFA) [Bacillus subtilis strain SRCM125727]
GTGAATGTGCCAGTTGAAAAAAACAGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATAA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATGTAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAAAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATCTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAAGTGCAAACGGGTGTA
CTAGGAGCGGAATCGTCTATCTTTACTGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Helicase conserved C-terminal domain (Helicase_C)

(326-423)

Type III restriction enzyme, res subunit (ResIII)

(120-268)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

100

100

1