Detailed information NTG0779274

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: POF62_RS05610
• Genome accession: NZ_CP116707
• Coordinates: 1074403..1075245 (-)
• Length: 280 a.a.
• NCBI ID: WP_001015245.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain A909 sCas9revertant
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1069403..1080245

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF62_RS05600 (POF62_05600)	-	1071350..1072051 (-)	702	WP_000120355.1	ABC transporter ATP-binding protein	-
POF62_RS05605 (POF62_05605)	topA	1072188..1074308 (-)	2121	WP_000246611.1	type I DNA topoisomerase	-
POF62_RS05610 (POF62_05610)	dprA	1074403..1075245 (-)	843	WP_001015245.1	DNA-processing protein DprA	Machinery gene
POF62_RS05615 (POF62_05615)	-	1075383..1076411 (-)	1029	WP_000162771.1	siderophore ABC transporter substrate-binding protein	-
POF62_RS05620 (POF62_05620)	-	1076473..1077234 (-)	762	WP_000614752.1	ATP-binding cassette domain-containing protein	-
POF62_RS05625 (POF62_05625)	-	1077231..1078205 (-)	975	WP_000588585.1	iron chelate uptake ABC transporter family permease subunit	-
POF62_RS05630 (POF62_05630)	-	1078202..1079164 (-)	963	WP_000735584.1	iron chelate uptake ABC transporter family permease subunit	-
POF62_RS05635 (POF62_05635)	-	1079403..1079951 (-)	549	WP_000136503.1	sugar O-acetyltransferase	-

Sequence

Protein

• Length: 280 a.a.
• Molecular weight: 31329.36 Da
• Isoelectric Point: 9.2263

>NTDB_id=779274 POF62_RS05610 WP_001015245.1 1074403..1075245(-) (dprA) [Streptococcus agalactiae strain A909 sCas9revertant]
MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSKCKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNY
PLELKEIYNPPVLLFYQGNIELLSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARDIDTAAHVSALKNGGSS
IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFTGEQPLKFHFPERNRIIAGLCQGIVVAEAKMRSGSLITCERALEEG
REVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDILSEYQ

Nucleotide

• Length: 843 bp

>NTDB_id=779274 POF62_RS05610 WP_001015245.1 1074403..1075245(-) (dprA) [Streptococcus agalactiae strain A909 sCas9revertant]
ATGAATCATTTTGAACTATTTAAGCTTAAAAAAGCTGGATTAACAAATCTTAATATCAACAATATTATCAACTATCTCAA
AAAGAATAGTTTAACTTCTCTATCTGTTCGCAATATGGCCGTAGTATCAAAATGTAAAAATCCTACTTTCTTTATAGAAA
ATTATAAACAGCTAGACCTTAAAAAACTTCGACAAGAATTCAAAAAATTTCCAGTTCTATCGATTTTGGATTCTAACTAT
CCTTTAGAGTTAAAAGAAATATATAATCCACCAGTTCTACTTTTTTATCAGGGTAATATTGAACTTCTATCTAAACCTAA
ATTAGCTGTAGTGGGGGCAAGACAGGCATCTCAGATAGGTTGTCAGTCTGTTAAAAAGATTATCAAGGAGACTAACAATC
AATTTGTTATCGTAAGTGGTTTAGCGCGTGACATTGATACAGCAGCACATGTTAGTGCTTTAAAAAATGGCGGCAGTAGT
ATAGCTGTTATCGGGAGTGGTTTAGATGTTTATTATCCAACGGAGAATAAGAAACTTCAAGAATATATGTCATATAATCA
TCTCGTATTATCAGAATATTTTACCGGAGAACAACCCTTGAAATTTCATTTCCCCGAACGTAACCGTATTATTGCAGGGC
TGTGTCAAGGTATTGTGGTTGCCGAAGCTAAGATGAGATCTGGAAGTTTAATTACCTGTGAAAGAGCATTAGAAGAGGGA
CGAGAAGTTTTTGCCATTCCCGGAAATATTATCGATGGCAAATCAGATGGATGCCACCATCTTATCCAAGAAGGAGCTAA
ATGCATTATTTCAGGAAAAGATATCCTTTCTGAATATCAGTAG

Domains

Predicted by InterproScan.

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-63)

• DNA recombination-mediator protein A (DNA_processg_A) (69-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	69.892	99.643	0.696
dprA/cilB/dalA	Streptococcus mitis SK321	62.143	100	0.621
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	61.429	100	0.614
dprA/cilB/dalA	Streptococcus pneumoniae D39	61.429	100	0.614
dprA/cilB/dalA	Streptococcus pneumoniae R6	61.429	100	0.614
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	61.429	100	0.614
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	61.071	100	0.611
dprA	Lactococcus lactis subsp. cremoris KW2	60.932	99.643	0.607
dprA	Latilactobacillus sakei subsp. sakei 23K	41.833	89.643	0.375