Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   POF65_RS02035 Genome accession   NZ_CP116706
Coordinates   355748..357037 (+) Length   429 a.a.
NCBI ID   WP_000432961.1    Uniprot ID   A0AAV3JP26
Organism   Streptococcus agalactiae strain A909 sCas9     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 350748..362037
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  POF65_RS02015 (POF65_02015) - 352055..352723 (+) 669 WP_000394891.1 fructose-6-phosphate aldolase -
  POF65_RS02020 (POF65_02020) - 352791..353879 (+) 1089 WP_000862118.1 glycerol dehydrogenase -
  POF65_RS02025 (POF65_02025) cysK 354031..354957 (-) 927 WP_000036948.1 cysteine synthase A -
  POF65_RS02030 (POF65_02030) - 355048..355692 (-) 645 WP_001108147.1 YigZ family protein -
  POF65_RS02035 (POF65_02035) comFA/cflA 355748..357037 (+) 1290 WP_000432961.1 DEAD/DEAH box helicase Machinery gene
  POF65_RS02040 (POF65_02040) - 357037..357702 (+) 666 WP_000128297.1 ComF family protein -
  POF65_RS02045 (POF65_02045) raiA 357779..358333 (+) 555 WP_000599094.1 ribosome-associated translation inhibitor RaiA -

Sequence

Protein


Download         Length: 429 a.a.        Molecular weight: 48760.56 Da        Isoelectric Point: 9.9154

>NTDB_id=779201 POF65_RS02035 WP_000432961.1 355748..357037(+) (comFA/cflA) [Streptococcus agalactiae strain A909 sCas9]
MENYLGRLWTKAQLSEQLRKIAISLPSFIKKGSDYICTRCSSSVAKNCQLPTGNYYCRECIVFGRVTSNENLYYFPQKTF
SKTNSLKWKGELTPYQNEVSEELLKGISSKENLLVHAVTGAGKTEMIYHSVAKVIDTGGSVCIASPRIDVCLELYKRLSN
DFRCAITLMHGESPSYQRSPLTIATTHQLLKFYHAFDLLIVDEVDAFPYVDNPILYQGVKQALKENGTSIFLTATSTTEL
ERKVARKELKKLHLARRFHANPLVIPEMVWVSGIQKSLQTQKLPPKLYQLINKQRQTRYPLLLFFPHISEGQVFTEILRQ
AFPMEKIGFVSSKSTSRLKLVQDFRDNKLSILVSTTILERGVTFPSVDVFVIQANHHLFTKSSLVQISGRVGRALERPEG
LLYFLHDGKSKSMHQAIKEIKNMNHIGGF

Nucleotide


Download         Length: 1290 bp        

>NTDB_id=779201 POF65_RS02035 WP_000432961.1 355748..357037(+) (comFA/cflA) [Streptococcus agalactiae strain A909 sCas9]
ATGGAAAATTATTTAGGTAGGCTTTGGACAAAAGCACAATTATCTGAGCAACTTAGAAAAATTGCAATTAGCTTGCCATC
TTTTATAAAGAAAGGAAGCGACTATATTTGTACCCGATGCTCTAGTTCTGTTGCTAAAAATTGCCAATTACCAACTGGAA
ATTATTATTGTAGAGAATGTATAGTTTTTGGAAGAGTTACTAGTAATGAAAATTTATATTATTTCCCACAAAAAACATTT
TCTAAAACGAATTCTTTAAAATGGAAAGGGGAACTGACACCTTATCAAAATGAGGTTTCAGAAGAGTTGTTGAAAGGTAT
TTCAAGTAAGGAAAATCTTCTTGTTCATGCTGTGACAGGGGCTGGTAAAACAGAAATGATATACCACTCTGTCGCCAAAG
TAATTGATACTGGAGGAAGCGTTTGTATTGCCAGTCCTAGAATTGATGTTTGTTTAGAATTGTATAAGCGACTTTCAAAC
GATTTTAGATGTGCCATAACATTAATGCATGGAGAATCTCCTTCTTATCAGAGATCCCCTTTAACTATTGCCACAACACA
TCAATTGTTAAAATTTTATCATGCTTTTGATTTGCTAATTGTTGATGAAGTTGATGCTTTTCCGTATGTCGACAACCCGA
TACTATACCAGGGTGTAAAACAGGCCTTAAAGGAAAACGGAACAAGTATTTTTCTGACTGCAACTTCAACGACTGAGTTA
GAAAGAAAAGTTGCAAGAAAAGAACTAAAAAAATTGCATCTAGCTAGGAGATTTCATGCCAATCCGTTAGTGATACCCGA
GATGGTTTGGGTAAGTGGTATCCAGAAAAGCTTGCAGACACAAAAACTACCTCCCAAATTATATCAGTTGATAAATAAAC
AGCGACAAACGAGATATCCTTTGTTATTATTTTTCCCCCATATTTCAGAAGGTCAGGTATTCACCGAAATTTTAAGACAA
GCCTTTCCTATGGAAAAAATTGGTTTTGTTTCAAGTAAGAGCACATCTAGGTTAAAGTTAGTGCAAGATTTTCGAGATAA
CAAATTGTCTATCCTAGTGTCAACTACCATTCTAGAGAGAGGAGTAACTTTCCCTAGCGTCGACGTTTTTGTTATACAAG
CAAATCATCATTTGTTTACGAAAAGTTCCTTGGTACAAATTTCAGGAAGAGTTGGAAGAGCATTAGAAAGGCCCGAAGGG
CTCCTTTACTTCCTTCATGATGGGAAAAGTAAATCGATGCACCAAGCTATAAAGGAGATTAAAAACATGAATCATATAGG
AGGATTTTAA

Domains

Predicted by InterproScan.

DEAD/DEAH box helicase (DEAD)

(93-240)

Helicase conserved C-terminal domain (Helicase_C)

(286-394)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

60.89

99.534

0.606
  comFA/cflA Streptococcus pneumoniae Rx1

60.422

99.534

0.601
  comFA/cflA Streptococcus pneumoniae D39

60.422

99.534

0.601
  comFA/cflA Streptococcus pneumoniae R6

60.422

99.534

0.601
  comFA/cflA Streptococcus mitis SK321

60.422

99.534

0.601
  comFA/cflA Streptococcus pneumoniae TIGR4

60.422

99.534

0.601
  comFA Lactococcus lactis subsp. cremoris KW2

49.746

91.841

0.457
  comFA Bacillus subtilis subsp. subtilis str. 168

39.312

94.872

0.373
  comFA Latilactobacillus sakei subsp. sakei 23K

36.552

100

0.371