Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   PML25_RS18275 Genome accession   NZ_CP116672
Coordinates   4353819..4354952 (+) Length   377 a.a.
NCBI ID   WP_168957167.1    Uniprot ID   A0A6V7EL88
Organism   Xanthomonas hortorum pv. pelargonii strain 305     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4348819..4359952
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML25_RS18250 (PML25_18250) - 4349022..4350221 (-) 1200 WP_168957164.1 cystathionine gamma-synthase -
  PML25_RS18255 (PML25_18255) - 4350309..4351679 (-) 1371 WP_074056614.1 pyridoxal-phosphate dependent enzyme -
  PML25_RS18260 (PML25_18260) - 4351898..4352116 (-) 219 WP_168957165.1 YdcH family protein -
  PML25_RS18265 (PML25_18265) - 4352450..4353340 (-) 891 WP_168957166.1 hypothetical protein -
  PML25_RS18270 (PML25_18270) - 4353337..4353720 (-) 384 WP_006449646.1 DUF4398 domain-containing protein -
  PML25_RS18275 (PML25_18275) pilU 4353819..4354952 (+) 1134 WP_168957167.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  PML25_RS18280 (PML25_18280) maiA 4355469..4356131 (-) 663 WP_168957168.1 maleylacetoacetate isomerase -
  PML25_RS18285 (PML25_18285) - 4356187..4357245 (-) 1059 WP_180336499.1 fumarylacetoacetate hydrolase family protein -
  PML25_RS18290 (PML25_18290) - 4357553..4358941 (+) 1389 WP_168957170.1 DEAD/DEAH box helicase -

Sequence

Protein


Download         Length: 377 a.a.        Molecular weight: 41785.03 Da        Isoelectric Point: 6.1395

>NTDB_id=779000 PML25_RS18275 WP_168957167.1 4353819..4354952(+) (pilU) [Xanthomonas hortorum pv. pelargonii strain 305]
MDIGYFLKLMTEKNASDMFLTTGAPVYIKIEGKLYPLGNTGLPPGMVKKIAYSLMDEGQVPQFERDLELNMAISLPDAGR
FRVNVFKQRGEAGMVIRAIRSRIPSIEELNLPQVLKDVIMTPRGLVLLVGSTGSGKSTSLASMIDHRNSTTTGHILTIED
PIEYLHKHKMSIVNQREVGLDTHTFQSALKNAMREAPDVILIGEILDAETMEAAIAFAETGHLCLATLHSNNADQTIERI
LNFFPESAHKNVLMNLALNLRAVVSQRLVKGQDGRRRPATEVLLNTPMIRDLLRRGQVHEVKAAMEESLEEGMQTFDQCL
FRMVKSGEIEQEEALRAADSRDGLALKFRLSEGGSGEHDPYADYETAATPKISHGFV

Nucleotide


Download         Length: 1134 bp        

>NTDB_id=779000 PML25_RS18275 WP_168957167.1 4353819..4354952(+) (pilU) [Xanthomonas hortorum pv. pelargonii strain 305]
ATGGATATCGGCTACTTCCTGAAGCTGATGACCGAAAAGAATGCCTCGGACATGTTTTTGACCACCGGCGCGCCGGTGTA
CATCAAGATCGAAGGCAAGCTCTACCCGCTCGGCAATACGGGGTTGCCGCCGGGGATGGTCAAGAAAATCGCCTACTCGT
TGATGGACGAGGGCCAGGTGCCGCAGTTCGAGCGCGACCTGGAACTCAACATGGCCATCTCGCTGCCCGACGCCGGACGC
TTCCGTGTGAACGTGTTCAAGCAGCGCGGCGAAGCAGGCATGGTGATCCGCGCCATCCGCAGCCGGATCCCGAGCATCGA
AGAGCTCAATCTGCCGCAGGTGCTCAAGGACGTCATCATGACGCCGCGCGGCCTGGTGCTGCTGGTCGGCTCGACCGGTT
CCGGCAAGTCGACCTCGCTGGCCTCGATGATCGACCACCGCAACAGCACCACCACCGGGCACATCCTCACCATCGAGGAC
CCGATCGAATACCTGCACAAGCACAAGATGTCGATCGTGAACCAGCGCGAAGTCGGCCTGGACACCCACACCTTCCAGAG
CGCGCTGAAGAACGCGATGCGCGAGGCGCCGGACGTGATCCTGATCGGCGAAATCCTGGATGCCGAGACCATGGAGGCGG
CGATCGCCTTCGCCGAGACCGGCCACCTGTGCCTGGCAACACTGCACTCCAACAATGCCGACCAGACCATCGAGCGCATT
CTCAATTTCTTCCCGGAAAGCGCGCACAAGAACGTGCTGATGAATCTGGCGCTGAATTTGCGTGCGGTGGTCTCCCAGCG
CCTGGTCAAGGGCCAGGACGGACGCCGCCGCCCCGCCACCGAAGTCTTGCTCAACACCCCGATGATTCGCGACCTACTGC
GACGCGGCCAGGTGCACGAGGTCAAGGCGGCGATGGAAGAATCGCTGGAAGAAGGCATGCAGACCTTCGACCAATGCCTG
TTCCGCATGGTCAAGTCAGGCGAGATCGAGCAGGAGGAAGCGCTGCGCGCCGCCGACTCGCGCGATGGTCTGGCGCTGAA
GTTCCGCCTGTCCGAAGGCGGCTCCGGCGAGCACGACCCGTATGCGGATTACGAAACGGCCGCAACGCCCAAGATTAGCC
ACGGGTTTGTTTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(77-270)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6V7EL88

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

52.925

95.225

0.504
  pilU Vibrio cholerae strain A1552

49.167

95.491

0.469
  pilU Acinetobacter baylyi ADP1

49.435

93.899

0.464
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.455

100

0.44
  pilT Pseudomonas aeruginosa PAK

42.899

89.655

0.385
  pilT Legionella pneumophila strain Lp02

41.42

89.655

0.371
  pilT Acinetobacter baumannii strain A118

41.42

89.655

0.371
  pilT Acinetobacter nosocomialis M2

41.42

89.655

0.371
  pilT Legionella pneumophila strain ERS1305867

41.42

89.655

0.371
  pilT Acinetobacter baumannii D1279779

41.42

89.655

0.371
  pilT Acinetobacter baylyi ADP1

40.882

90.186

0.369
  pilT Neisseria gonorrhoeae MS11

39.655

92.308

0.366
  pilT Neisseria meningitidis 8013

39.655

92.308

0.366
  pilT Pseudomonas stutzeri DSM 10701

41.317

88.594

0.366