Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   PML73_RS00380 Genome accession   NZ_CP116571
Coordinates   76548..77075 (+) Length   175 a.a.
NCBI ID   WP_271865440.1    Uniprot ID   -
Organism   Enterococcus faecalis strain K190-1     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 65628..111663 76548..77075 within 0


Gene organization within MGE regions


Location: 65628..111663
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PML73_RS00295 (PML73_00295) - 67551..68900 (-) 1350 WP_002411357.1 recombinase family protein -
  PML73_RS00300 (PML73_00300) - 68929..69111 (-) 183 WP_002411356.1 hypothetical protein -
  PML73_RS00305 (PML73_00305) - 69276..69992 (-) 717 WP_229017980.1 XRE family transcriptional regulator -
  PML73_RS00310 (PML73_00310) - 70138..70344 (+) 207 WP_002411354.1 helix-turn-helix domain-containing protein -
  PML73_RS00315 (PML73_00315) - 70382..70543 (+) 162 WP_002411353.1 hypothetical protein -
  PML73_RS00320 (PML73_00320) - 70601..70771 (+) 171 WP_002357333.1 hypothetical protein -
  PML73_RS00325 (PML73_00325) - 70765..70986 (-) 222 WP_229018025.1 hypothetical protein -
  PML73_RS00330 (PML73_00330) - 71054..71269 (+) 216 WP_002357335.1 hypothetical protein -
  PML73_RS00335 (PML73_00335) - 71538..72248 (+) 711 WP_010821530.1 Rha family transcriptional regulator -
  PML73_RS00340 (PML73_00340) - 72259..72444 (+) 186 WP_002411350.1 hypothetical protein -
  PML73_RS00345 (PML73_00345) - 72456..72629 (+) 174 WP_002381626.1 hypothetical protein -
  PML73_RS00350 (PML73_00350) - 72737..73039 (+) 303 WP_083578494.1 hypothetical protein -
  PML73_RS00355 (PML73_00355) - 73125..73868 (+) 744 WP_002401996.1 ERF family protein -
  PML73_RS00360 (PML73_00360) - 73868..74806 (+) 939 WP_160816517.1 DUF1351 domain-containing protein -
  PML73_RS00365 (PML73_00365) - 74803..75444 (+) 642 WP_160816516.1 putative HNHc nuclease -
  PML73_RS00370 (PML73_00370) - 75448..76245 (+) 798 WP_010784194.1 helix-turn-helix domain-containing protein -
  PML73_RS00375 (PML73_00375) - 76250..76558 (+) 309 WP_010822654.1 MazG-like family protein -
  PML73_RS00380 (PML73_00380) ssb 76548..77075 (+) 528 WP_271865440.1 single-stranded DNA-binding protein Machinery gene
  PML73_RS00385 (PML73_00385) - 77087..77416 (+) 330 WP_010821535.1 hypothetical protein -
  PML73_RS00390 (PML73_00390) - 77436..77642 (+) 207 WP_002395701.1 hypothetical protein -
  PML73_RS00395 (PML73_00395) - 77695..78435 (+) 741 WP_002406059.1 hypothetical protein -
  PML73_RS00400 (PML73_00400) - 78492..79145 (+) 654 WP_002406058.1 N-6 DNA methylase -
  PML73_RS00405 (PML73_00405) - 79142..80086 (+) 945 WP_002406057.1 site-specific integrase -
  PML73_RS00410 (PML73_00410) - 80099..80671 (+) 573 WP_002406056.1 hypothetical protein -
  PML73_RS00415 (PML73_00415) - 80655..81035 (+) 381 WP_271865511.1 YopX family protein -
  PML73_RS00420 (PML73_00420) - 81036..81368 (+) 333 WP_233060211.1 hypothetical protein -
  PML73_RS00425 (PML73_00425) - 81368..81529 (+) 162 WP_271865446.1 hypothetical protein -
  PML73_RS00430 (PML73_00430) - 81526..81804 (+) 279 WP_271865448.1 hypothetical protein -
  PML73_RS00435 (PML73_00435) - 81816..82034 (+) 219 WP_010774592.1 hypothetical protein -
  PML73_RS00440 (PML73_00440) - 82031..82588 (+) 558 WP_002416044.1 DUF1642 domain-containing protein -
  PML73_RS00445 (PML73_00445) - 82771..82980 (+) 210 WP_002402424.1 hypothetical protein -
  PML73_RS00450 (PML73_00450) - 82981..83388 (+) 408 WP_002416043.1 transcriptional regulator -
  PML73_RS00455 (PML73_00455) - 83632..83901 (+) 270 WP_104807408.1 hypothetical protein -
  PML73_RS00460 (PML73_00460) - 83937..84074 (-) 138 WP_010773976.1 zinc ribbon-containing protein -
  PML73_RS00470 (PML73_00470) - 84602..85435 (+) 834 WP_271865513.1 terminase small subunit -
  PML73_RS00475 (PML73_00475) - 85425..86714 (+) 1290 WP_185116382.1 PBSX family phage terminase large subunit -
  PML73_RS00480 (PML73_00480) - 86730..88229 (+) 1500 WP_148251867.1 phage portal protein -
  PML73_RS00485 (PML73_00485) - 88229..89386 (+) 1158 WP_002411227.1 phage minor capsid protein -
  PML73_RS00490 (PML73_00490) - 89470..89733 (+) 264 WP_025188247.1 DUF6275 family protein -
  PML73_RS00495 (PML73_00495) - 89839..90393 (+) 555 WP_010784200.1 phage scaffolding protein -
  PML73_RS00500 (PML73_00500) - 90410..91276 (+) 867 WP_002357374.1 hypothetical protein -
  PML73_RS00505 (PML73_00505) - 91374..91778 (+) 405 WP_010784201.1 hypothetical protein -
  PML73_RS00510 (PML73_00510) - 91772..92113 (+) 342 WP_002411222.1 putative minor capsid protein -
  PML73_RS00515 (PML73_00515) - 92113..92439 (+) 327 WP_002411221.1 minor capsid protein -
  PML73_RS00520 (PML73_00520) - 92439..92837 (+) 399 WP_002411220.1 minor capsid protein -
  PML73_RS00525 (PML73_00525) - 92837..93316 (+) 480 WP_002411219.1 phage tail tube protein -
  PML73_RS00530 (PML73_00530) - 93387..93839 (+) 453 WP_010784203.1 hypothetical protein -
  PML73_RS00535 (PML73_00535) - 93892..94311 (+) 420 WP_002411217.1 DUF6673 family protein -
  PML73_RS00540 (PML73_00540) - 94317..94889 (+) 573 WP_010784204.1 Gp15 family bacteriophage protein -
  PML73_RS00545 (PML73_00545) - 94882..98202 (+) 3321 WP_104855461.1 hypothetical protein -
  PML73_RS00550 (PML73_00550) - 98202..98963 (+) 762 WP_010826290.1 distal tail protein Dit -
  PML73_RS00555 (PML73_00555) - 98960..101227 (+) 2268 WP_271865451.1 phage tail spike protein -
  PML73_RS00560 (PML73_00560) - 101227..103014 (+) 1788 WP_271865453.1 BppU family phage baseplate upper protein -
  PML73_RS00565 (PML73_00565) - 103088..103309 (+) 222 WP_334312076.1 hypothetical protein -
  PML73_RS00570 (PML73_00570) - 103302..103538 (+) 237 WP_002411209.1 phage holin -
  PML73_RS00575 (PML73_00575) - 103535..104620 (+) 1086 WP_010731631.1 SH3 domain-containing protein -
  PML73_RS00580 (PML73_00580) - 105014..105547 (+) 534 WP_002402066.1 PBECR4 domain-containing protein -
  PML73_RS00590 (PML73_00590) - 105702..109577 (+) 3876 Protein_115 5'-nucleotidase C-terminal domain-containing protein -
  PML73_RS00595 (PML73_00595) - 109713..111392 (+) 1680 WP_002361487.1 peptide ABC transporter substrate-binding protein -
  PML73_RS00600 (PML73_00600) - 111439..111663 (-) 225 WP_002356070.1 DUF896 domain-containing protein -

Sequence


Protein


Download         Length: 175 a.a.        Molecular weight: 19285.11 Da        Isoelectric Point: 4.7078

>NTDB_id=778459 PML73_RS00380 WP_271865440.1 76548..77075(+) (ssb) [Enterococcus faecalis strain K190-1]
MINNVVLVGRLTKDPDLRYTASGSAVGSFTLAVNRNFTNQNGEREADFINCVIWRKPAETMANYARKGTLLGVVGRIQTR
NYDNQQGQRVYVTEVVCESFQLLEAKSANENRNSIQTSQNSGTSVQNNFESNYATNQNKGLNQQNNSQQMSFGGDVDPFA
GAGNSIDISDDDLPF

Nucleotide


Download         Length: 528 bp        

>NTDB_id=778459 PML73_RS00380 WP_271865440.1 76548..77075(+) (ssb) [Enterococcus faecalis strain K190-1]
ATGATAAATAATGTGGTATTAGTCGGAAGATTGACAAAAGATCCTGATTTACGCTACACCGCAAGTGGTTCTGCAGTTGG
AAGCTTTACTCTTGCTGTGAACCGTAACTTTACAAACCAAAACGGCGAACGAGAAGCGGATTTTATCAACTGTGTAATTT
GGCGTAAGCCTGCTGAAACAATGGCTAATTATGCTCGTAAAGGAACATTATTAGGAGTTGTTGGCAGAATTCAAACTCGT
AATTATGACAACCAACAAGGCCAACGTGTCTATGTGACTGAAGTTGTTTGCGAGAGTTTCCAATTATTAGAGGCAAAAAG
CGCCAATGAGAATAGAAATAGCATTCAGACTTCACAGAACAGCGGTACAAGTGTTCAAAACAATTTCGAGAGTAATTATG
CCACAAATCAAAATAAAGGCTTAAATCAGCAAAATAACAGCCAACAAATGTCGTTTGGTGGAGATGTAGATCCGTTCGCA
GGCGCAGGTAATTCAATCGACATTAGCGACGATGATCTGCCGTTCTAG


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

59.091

100

0.594

  ssbA Bacillus subtilis subsp. subtilis str. 168

57.143

100

0.571

  ssbB Bacillus subtilis subsp. subtilis str. 168

59.434

60.571

0.36