Detailed information    

insolico Bioinformatically predicted

Overview


Name   phrF   Type   Regulator
Locus tag   PIB33_RS20425 Genome accession   NZ_CP116391
Coordinates   3863235..3863354 (+) Length   39 a.a.
NCBI ID   WP_009968329.1    Uniprot ID   A0A199WEM2
Organism   Bacillus subtilis strain GXD-20     
Function   antagonize RapF (predicted from homology)   
Competence regulation

Genomic Context


Location: 3858235..3868354
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS20405 (PIB33_20405) albG 3858399..3859100 (+) 702 WP_015250988.1 antilisterial bacteriocin subtilosin biosynthesis protein AlbG -
  PIB33_RS20410 (PIB33_20410) ywhL 3859106..3860482 (-) 1377 WP_032722701.1 YncE family protein -
  PIB33_RS20415 (PIB33_20415) ywhK 3860521..3861876 (-) 1356 WP_032722703.1 YncE family protein -
  PIB33_RS22320 - 3861873..3862103 (-) 231 WP_009968328.1 hypothetical protein -
  PIB33_RS20420 (PIB33_20420) rapF 3862106..3863251 (+) 1146 WP_003227549.1 response regulator aspartate phosphatase RapF Regulator
  PIB33_RS20425 (PIB33_20425) phrF 3863235..3863354 (+) 120 WP_009968329.1 phosphatase RapF inhibitor PhrF Regulator
  PIB33_RS20430 (PIB33_20430) - 3863468..3863918 (+) 451 Protein_3970 YbaK/EbsC family protein -
  PIB33_RS20435 (PIB33_20435) speB 3863950..3864822 (-) 873 WP_032722706.1 agmatinase -
  PIB33_RS20440 (PIB33_20440) speE 3864883..3865713 (-) 831 WP_003227543.1 spermidine synthase -
  PIB33_RS20445 (PIB33_20445) pbpG 3865915..3867990 (+) 2076 WP_003227540.1 transglycosylase domain-containing protein -

Sequence


Protein


Download         Length: 39 a.a.        Molecular weight: 4199.10 Da        Isoelectric Point: 10.4997

>NTDB_id=777056 PIB33_RS20425 WP_009968329.1 3863235..3863354(+) (phrF) [Bacillus subtilis strain GXD-20]
MKLKSKLLLSCLALSTVFVATTIANAPTHQIEVAQRGMI

Nucleotide


Download         Length: 120 bp        

>NTDB_id=777056 PIB33_RS20425 WP_009968329.1 3863235..3863354(+) (phrF) [Bacillus subtilis strain GXD-20]
ATGAAATTGAAGTCTAAACTATTACTCTCTTGTCTGGCTCTAAGCACTGTGTTCGTGGCAACAACTATTGCAAATGCACC
TACACACCAAATTGAAGTTGCACAACGAGGAATGATTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A199WEM2

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  phrF Bacillus subtilis subsp. subtilis str. 168

100

100

1

  phrC Bacillus subtilis subsp. subtilis str. 168

48.718

100

0.487