Detailed information NTG0777055

Bioinformatically predicted

Overview

• Name: rapF
• Type: Regulator
• Locus tag: PIB33_RS20420
• Genome accession: NZ_CP116391
• Coordinates: 3862106..3863251 (+)
• Length: 381 a.a.
• NCBI ID: WP_003227549.1
• Uniprot ID: -
• Organism: Bacillus subtilis strain GXD-20
• Function: inhibit the DNA-binding function of ComA (predicted from homology) Competence regulation

Genomic Context

Location: 3857106..3868251

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PIB33_RS20400 (PIB33_20400)	albF	3857122..3858402 (+)	1281	WP_032722698.1	pitrilysin family protein	-
PIB33_RS20405 (PIB33_20405)	albG	3858399..3859100 (+)	702	WP_015250988.1	antilisterial bacteriocin subtilosin biosynthesis protein AlbG	-
PIB33_RS20410 (PIB33_20410)	ywhL	3859106..3860482 (-)	1377	WP_032722701.1	YncE family protein	-
PIB33_RS20415 (PIB33_20415)	ywhK	3860521..3861876 (-)	1356	WP_032722703.1	YncE family protein	-
PIB33_RS22320	-	3861873..3862103 (-)	231	WP_009968328.1	hypothetical protein	-
PIB33_RS20420 (PIB33_20420)	rapF	3862106..3863251 (+)	1146	WP_003227549.1	response regulator aspartate phosphatase RapF	Regulator
PIB33_RS20425 (PIB33_20425)	phrF	3863235..3863354 (+)	120	WP_009968329.1	phosphatase RapF inhibitor PhrF	Regulator
PIB33_RS20430 (PIB33_20430)	-	3863468..3863918 (+)	451	Protein_3970	YbaK/EbsC family protein	-
PIB33_RS20435 (PIB33_20435)	speB	3863950..3864822 (-)	873	WP_032722706.1	agmatinase	-
PIB33_RS20440 (PIB33_20440)	speE	3864883..3865713 (-)	831	WP_003227543.1	spermidine synthase	-
PIB33_RS20445 (PIB33_20445)	pbpG	3865915..3867990 (+)	2076	WP_003227540.1	transglycosylase domain-containing protein	-

Sequence

Protein

• Length: 381 a.a.
• Molecular weight: 45609.09 Da
• Isoelectric Point: 5.1503

>NTDB_id=777055 PIB33_RS20420 WP_003227549.1 3862106..3863251(+) (rapF) [Bacillus subtilis strain GXD-20]
MTGVISSSSIGEKINEWYMYIRRFSIPDAEYLRREIKQELDQMEEDQDLHLYYSLMEFRHNLMLEYLEPLEKMRIEEQPR
LSDLLLEIDKKQARLTGLLEYYFNFFRGMYELDQREYLSAIKFFKKAESKLIFVKDRIEKAEFFFKMSESYYYMKQTYFS
MDYARQAYEIYKEHEAYNIRLLQCHSLFATNFLDLKQYEDAISHFQKAYSMAEAEKQPQLMGRTLYNIGLCKNSQSQYED
AIPYFKRAIAVFEESNILPSLPQAYFLITQIHYKLGKIDKAHEYHSKGMAYSQKAGDVIYLSEFEFLKSLYLSGPDEEAI
QGFFDFLESKMLYADLEDFAIDVAKYYHERKNFQKASAYFLKVEQVRQLIQGGVSLYEIEV

Nucleotide

• Length: 1146 bp

>NTDB_id=777055 PIB33_RS20420 WP_003227549.1 3862106..3863251(+) (rapF) [Bacillus subtilis strain GXD-20]
GTGACAGGTGTCATATCTTCTTCTTCCATCGGAGAAAAGATTAACGAATGGTATATGTACATACGCCGATTCAGCATACC
CGATGCAGAATATTTGCGACGAGAAATCAAGCAAGAGCTGGATCAAATGGAAGAAGATCAAGACCTTCATTTGTACTATT
CACTGATGGAGTTTCGGCACAACCTAATGCTTGAGTACCTTGAACCGTTAGAAAAAATGAGGATTGAGGAACAGCCGAGA
CTGTCTGATCTGCTGCTTGAGATTGATAAAAAACAGGCTCGTTTAACTGGTCTGCTTGAGTACTATTTTAACTTCTTCAG
AGGCATGTACGAGCTGGACCAGCGGGAATATCTGTCGGCTATTAAATTTTTCAAAAAGGCCGAAAGCAAGCTGATATTCG
TTAAGGATCGGATAGAGAAAGCTGAGTTTTTCTTTAAGATGTCTGAATCTTATTACTATATGAAACAAACGTATTTTTCA
ATGGACTATGCACGGCAAGCATATGAAATATACAAAGAACATGAAGCTTATAATATAAGATTGCTGCAGTGTCATTCTTT
ATTTGCCACCAATTTTTTAGATTTAAAACAGTATGAGGATGCCATCTCACATTTTCAAAAAGCTTATTCTATGGCAGAAG
CTGAAAAGCAGCCCCAATTAATGGGGAGAACTTTGTACAATATCGGGCTTTGTAAAAACAGCCAAAGCCAATATGAGGAT
GCCATACCTTATTTCAAAAGAGCAATAGCTGTTTTTGAAGAATCAAATATTCTTCCTTCCTTACCTCAAGCGTATTTTTT
AATTACACAGATCCATTATAAATTAGGAAAAATAGATAAAGCTCATGAATATCATAGTAAGGGAATGGCTTATTCACAAA
AGGCCGGAGATGTAATATATTTATCAGAGTTTGAATTTTTGAAATCTTTATACTTATCAGGCCCGGATGAAGAAGCAATT
CAAGGATTTTTTGATTTTCTCGAAAGTAAAATGTTGTATGCTGATCTTGAAGATTTCGCTATTGATGTGGCAAAATATTA
TCATGAACGTAAAAATTTTCAAAAAGCTTCTGCTTATTTTTTGAAGGTGGAACAAGTAAGGCAACTTATTCAAGGAGGAG
TGAGTTTGTATGAAATTGAAGTCTAA

Domains

Predicted by InterproScan.

• response regulator aspartate phosphatase H, N terminal (RapH_N) (4-65)

• Tetratricopeptide repeat (TPR_12) (223-290)

• Anaphase-promoting complex, cyclosome, subunit 3 (ANAPC3) (132-208)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
rapF	Bacillus subtilis subsp. subtilis str. 168	100	100	1
rapC	Bacillus subtilis subsp. subtilis str. 168	57.368	99.738	0.572