Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA   Type   Machinery gene
Locus tag   PIB33_RS19365 Genome accession   NZ_CP116391
Coordinates   3658138..3659529 (-) Length   463 a.a.
NCBI ID   WP_032722611.1    Uniprot ID   -
Organism   Bacillus subtilis strain GXD-20     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3653138..3664529
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS19330 (PIB33_19330) flgL 3653310..3654206 (-) 897 WP_003228004.1 flagellar hook-associated protein FlgL -
  PIB33_RS19335 (PIB33_19335) flgK 3654217..3655740 (-) 1524 WP_003228001.1 flagellar hook-associated protein FlgK -
  PIB33_RS19340 (PIB33_19340) flgN 3655759..3656241 (-) 483 WP_032722609.1 flagellar protein FlgN -
  PIB33_RS19345 (PIB33_19345) flgM 3656257..3656523 (-) 267 WP_014478128.1 flagellar biosynthesis anti-sigma factor FlgM -
  PIB33_RS19350 (PIB33_19350) yvyF 3656604..3657023 (-) 420 WP_003227995.1 TIGR03826 family flagellar region protein -
  PIB33_RS19355 (PIB33_19355) comFC 3657096..3657818 (-) 723 WP_014481051.1 comF operon protein ComFC Machinery gene
  PIB33_RS19360 (PIB33_19360) comFB 3657782..3658078 (-) 297 WP_015483764.1 late competence protein ComFB -
  PIB33_RS19365 (PIB33_19365) comFA 3658138..3659529 (-) 1392 WP_032722611.1 ATP-dependent helicase ComFA Machinery gene
  PIB33_RS19370 (PIB33_19370) fakBA 3659635..3660480 (-) 846 WP_003244125.1 DegV family protein -
  PIB33_RS19375 (PIB33_19375) degU 3660578..3661267 (-) 690 WP_003219701.1 two-component system response regulator DegU Regulator
  PIB33_RS19380 (PIB33_19380) degS 3661350..3662507 (-) 1158 WP_003227983.1 two-component sensor histidine kinase DegS Regulator
  PIB33_RS19385 (PIB33_19385) yvyE 3662724..3663377 (+) 654 WP_003227979.1 YigZ family protein -

Sequence

Protein


Download         Length: 463 a.a.        Molecular weight: 52605.71 Da        Isoelectric Point: 10.0153

>NTDB_id=777049 PIB33_RS19365 WP_032722611.1 3658138..3659529(-) (comFA) [Bacillus subtilis strain GXD-20]
MNVPVEKNGSFSKELQQTLRSRHLLRTELSFSDEMIEWHIKNGYITAENSISMNKRRYRCNRCGQTDQRYFSFYHSSGKN
KLYCRSCVMMGRVSEEVPLYSWKEENESNWKSIKLTWDGKLSSGQQKAANVLIEAISKKEELLIWAVCGAGKTEMLFPGI
ESALNQGLRVCIATPRTDVVLELAPRLKAAFQGADISALYGGSDDKGRLSPLMISTTHQLLRYKDAIDVMIIDEVDAFPY
SADQTLQFAVQKARKKNSTLVYLSATPPKELKRKALNGQLHSVRIPARHHRKPLPEPRFVWCGNWKKKLNRNKIPPAVKR
WIEFHIKEGRPVFLFVPSVSILEKAAACFRGVHCRTASVHAEDKHRKEKVQQFRDGQLDLLITTTILERGVTVPKVQTGV
LGAESPIFTESALVQIAGRTGRHKEYADGDVIYFHFGKTKSMLDARKHIKEMNELAAKVECTD

Nucleotide


Download         Length: 1392 bp        

>NTDB_id=777049 PIB33_RS19365 WP_032722611.1 3658138..3659529(-) (comFA) [Bacillus subtilis strain GXD-20]
GTGAATGTGCCAGTTGAAAAAAACGGTTCCTTTTCAAAAGAATTGCAGCAGACGCTTCGAAGCCGTCATTTGCTCAGGAC
TGAGCTCTCATTTTCCGATGAGATGATTGAATGGCATATCAAGAATGGATATATCACTGCTGAAAATTCTATATCCATGA
ATAAACGGAGATATAGATGTAATAGGTGCGGACAAACTGATCAGCGGTATTTTTCTTTTTATCACTCATCTGGAAAGAAT
AAGCTGTATTGCCGTTCCTGTGTCATGATGGGCAGAGTGAGTGAGGAGGTTCCTTTATATTCATGGAAAGAGGAAAATGA
ATCAAACTGGAAGTCTATTAAGCTGACATGGGATGGCAAGCTTTCAAGCGGACAGCAAAAAGCCGCCAATGTTTTAATTG
AAGCAATATCAAAAAAAGAAGAGCTCCTCATCTGGGCGGTTTGCGGCGCTGGCAAAACAGAAATGCTGTTTCCTGGTATA
GAATCAGCGTTAAATCAAGGACTGCGTGTATGTATTGCAACACCTCGCACCGATGTTGTATTAGAGCTTGCTCCAAGACT
CAAGGCTGCCTTTCAGGGTGCTGACATTTCAGCGCTTTACGGAGGAAGCGATGACAAAGGGCGGCTATCTCCGCTTATGA
TTTCCACTACGCATCAGCTTTTGCGATATAAAGATGCAATCGATGTTATGATCATTGATGAAGTTGACGCTTTTCCATAT
TCTGCTGATCAAACCCTTCAATTCGCTGTTCAAAAAGCAAGAAAGAAAAACAGCACCCTCGTTTATTTAAGTGCAACACC
TCCTAAAGAATTAAAAAGAAAAGCACTGAACGGACAGTTACATTCAGTTCGCATCCCCGCAAGACACCACCGGAAACCTT
TACCCGAACCGCGCTTTGTATGGTGTGGAAACTGGAAGAAGAAATTAAACCGAAATAAAATTCCGCCAGCGGTGAAAAGA
TGGATAGAGTTTCATATAAAAGAAGGGAGGCCTGTTTTTTTATTCGTTCCTTCCGTTTCTATTCTGGAAAAGGCTGCTGC
GTGCTTTAGAGGTGTTCATTGCCGAACCGCATCTGTGCACGCGGAAGACAAGCATAGAAAGGAGAAAGTGCAGCAATTCA
GAGATGGTCAGCTCGATTTATTAATCACAACAACAATACTGGAAAGAGGCGTCACAGTCCCCAAGGTGCAAACGGGTGTA
CTAGGAGCGGAATCACCTATCTTTACGGAAAGCGCACTTGTTCAAATTGCAGGAAGAACCGGCCGGCATAAAGAATATGC
GGACGGCGATGTCATTTACTTTCACTTCGGCAAAACAAAGAGTATGCTCGATGCAAGAAAGCATATAAAAGAAATGAATG
AATTGGCAGCAAAAGTTGAATGTACAGACTAG

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(120-268)

Helicase conserved C-terminal domain (Helicase_C)

(327-423)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA Bacillus subtilis subsp. subtilis str. 168

98.92

100

0.989