Detailed information    

insolico Bioinformatically predicted

Overview

Name   recO   Type   Machinery gene
Locus tag   PIB33_RS13485 Genome accession   NZ_CP116391
Coordinates   2569846..2570613 (-) Length   255 a.a.
NCBI ID   WP_014477369.1    Uniprot ID   A0A0D1L7G7
Organism   Bacillus subtilis strain GXD-20     
Function   ssDNA annealing; plasmid transformation (predicted from homology)   
DNA processing Homologous recombination

Genomic Context

Location: 2564846..2575613
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS13465 (PIB33_13465) ppsR 2565021..2565833 (-) 813 WP_032722128.1 pyruvate, water dikinase regulatory protein -
  PIB33_RS13470 (PIB33_13470) ccpN 2565859..2566497 (-) 639 WP_003237058.1 transcriptional regulator CcpN -
  PIB33_RS13475 (PIB33_13475) glyS 2566630..2568669 (-) 2040 WP_032722129.1 glycine--tRNA ligase subunit beta -
  PIB33_RS13480 (PIB33_13480) glyQ 2568662..2569549 (-) 888 WP_003226213.1 glycine--tRNA ligase subunit alpha -
  PIB33_RS13485 (PIB33_13485) recO 2569846..2570613 (-) 768 WP_014477369.1 DNA repair protein RecO Machinery gene
  PIB33_RS13490 (PIB33_13490) yqzL 2570650..2570793 (-) 144 WP_003230053.1 YqzL family protein -
  PIB33_RS13495 (PIB33_13495) era 2570941..2571846 (-) 906 WP_003230051.1 GTPase Era -
  PIB33_RS13500 (PIB33_13500) - 2571827..2572237 (-) 411 WP_032722130.1 cytidine deaminase -
  PIB33_RS13505 (PIB33_13505) unpK 2572356..2572727 (-) 372 WP_015384119.1 diacylglycerol kinase family protein -
  PIB33_RS13510 (PIB33_13510) ybeY 2572708..2573181 (-) 474 WP_032722131.1 rRNA maturation RNase YbeY -
  PIB33_RS13515 (PIB33_13515) pgpH 2573182..2575317 (-) 2136 WP_010886570.1 cyclic-di-AMP phosphodiesterase PgpH -

Sequence

Protein


Download         Length: 255 a.a.        Molecular weight: 29342.05 Da        Isoelectric Point: 8.3080

>NTDB_id=777030 PIB33_RS13485 WP_014477369.1 2569846..2570613(-) (recO) [Bacillus subtilis strain GXD-20]
MLTKCEGIVLRTNDYGETNKIVTLLTREHGKIGVMARGAKKPNSRLSAVSQPFLYGSFLMQKTSGLGTLQQGEMILSMRG
IREDLFLTAYAAYIAELVDRGTEEKKPNPYLFEFILESLKQLNEGTDPDVITFIVQMKMLGVMGLYPELNHCVHCKSQDG
TFHFSVRDNGFICHRCFEKDPYRIPIKPQTARLLRLFYYFDLSRLGNVSLKEETKAELKQVIDLYYEEYSGLYLKSKRFL
DQMESMKHLMGENKS

Nucleotide


Download         Length: 768 bp        

>NTDB_id=777030 PIB33_RS13485 WP_014477369.1 2569846..2570613(-) (recO) [Bacillus subtilis strain GXD-20]
ATGCTGACAAAATGTGAAGGAATCGTTCTTCGCACAAATGATTACGGAGAGACGAATAAAATCGTAACTTTACTGACAAG
AGAACACGGAAAAATAGGTGTTATGGCACGAGGCGCCAAAAAACCGAACAGCCGTTTATCAGCAGTCAGCCAGCCCTTTT
TGTATGGTTCTTTCTTAATGCAAAAAACATCGGGCCTCGGAACGCTTCAGCAGGGGGAAATGATCCTCAGCATGAGAGGC
ATCAGGGAAGACTTGTTTTTAACAGCATATGCTGCCTATATTGCTGAGTTAGTCGACAGAGGCACAGAAGAAAAAAAGCC
AAATCCTTATTTATTTGAATTCATATTAGAGTCATTAAAACAGCTGAATGAAGGAACAGATCCGGATGTTATCACCTTCA
TTGTTCAAATGAAGATGCTCGGTGTCATGGGCCTGTATCCTGAGCTAAATCATTGTGTACATTGTAAAAGCCAAGACGGA
ACCTTTCATTTCTCTGTTCGTGATAATGGTTTTATATGCCACCGCTGTTTTGAAAAAGATCCTTACAGGATACCGATCAA
ACCGCAAACGGCAAGGCTGTTAAGACTCTTTTACTATTTTGATCTGTCGAGGCTCGGCAATGTGTCCTTAAAAGAGGAAA
CAAAAGCTGAATTAAAGCAAGTGATTGATTTATATTACGAAGAATATTCGGGATTGTATTTAAAATCAAAGCGCTTTTTA
GATCAAATGGAAAGCATGAAACATCTTATGGGTGAAAACAAAAGTTGA

Domains

Predicted by InterproScan.

Recombination protein O N terminal (RecO_N)

(1-75)

Recombination protein O C terminal (RecO_C)

(82-237)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D1L7G7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recO Bacillus subtilis subsp. subtilis str. 168

99.608

100

0.996