Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   PIB33_RS13655 Genome accession   NZ_CP116391
Coordinates   2601412..2602029 (-) Length   205 a.a.
NCBI ID   WP_032722140.1    Uniprot ID   -
Organism   Bacillus subtilis strain GXD-20     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2596412..2607029
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS13625 (PIB33_13625) rpsT 2596714..2596980 (+) 267 WP_003229989.1 30S ribosomal protein S20 -
  PIB33_RS13630 (PIB33_13630) holA 2596995..2598038 (-) 1044 WP_003229987.1 DNA polymerase III subunit delta -
  PIB33_RS13635 (PIB33_13635) - 2598078..2598227 (-) 150 WP_003229985.1 hypothetical protein -
  PIB33_RS13640 (PIB33_13640) yqzM 2598268..2598402 (+) 135 WP_003229983.1 YqzM family protein -
  PIB33_RS13645 (PIB33_13645) comEC 2598442..2600772 (-) 2331 WP_032722139.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  PIB33_RS13650 (PIB33_13650) comEB 2600776..2601345 (-) 570 WP_003229978.1 ComE operon protein 2 -
  PIB33_RS13655 (PIB33_13655) comEA 2601412..2602029 (-) 618 WP_032722140.1 competence protein ComEA Machinery gene
  PIB33_RS13660 (PIB33_13660) comER 2602113..2602934 (+) 822 WP_032722142.1 late competence protein ComER -
  PIB33_RS13665 (PIB33_13665) yqeM 2603000..2603743 (-) 744 WP_003229973.1 class I SAM-dependent methyltransferase -
  PIB33_RS13670 (PIB33_13670) rsfS 2603740..2604096 (-) 357 WP_014480315.1 ribosome silencing factor -
  PIB33_RS13675 (PIB33_13675) yqeK 2604114..2604674 (-) 561 WP_004399059.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  PIB33_RS13680 (PIB33_13680) nadD 2604664..2605233 (-) 570 WP_004398676.1 nicotinate-nucleotide adenylyltransferase -
  PIB33_RS13685 (PIB33_13685) yhbY 2605245..2605535 (-) 291 WP_003226133.1 ribosome assembly RNA-binding protein YhbY -
  PIB33_RS13690 (PIB33_13690) aroE 2605529..2606371 (-) 843 WP_003229967.1 shikimate dehydrogenase -

Sequence

Protein


Download         Length: 205 a.a.        Molecular weight: 21748.46 Da        Isoelectric Point: 4.8354

>NTDB_id=777033 PIB33_RS13655 WP_032722140.1 2601412..2602029(-) (comEA) [Bacillus subtilis strain GXD-20]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVRHPGVYELRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFPTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide


Download         Length: 618 bp        

>NTDB_id=777033 PIB33_RS13655 WP_032722140.1 2601412..2602029(-) (comEA) [Bacillus subtilis strain GXD-20]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAATGAAACAATTGTGATAGACATCAAAGGTGCTGTTCGGCATCCTGGCGTTTATGAATTGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAAGAAAACGGGCGTTTCCCAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(142-203)

SLBB domain (SLBB)

(63-118)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

98.537

100

0.985
  comEA Staphylococcus aureus MW2

36.364

100

0.39
  comEA Staphylococcus aureus N315

35.455

100

0.38