Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PIB33_RS13205 Genome accession   NZ_CP116391
Coordinates   2519904..2520974 (-) Length   356 a.a.
NCBI ID   WP_004399124.1    Uniprot ID   A0A809FNW6
Organism   Bacillus subtilis strain GXD-20     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2514904..2525974
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PIB33_RS13160 (PIB33_13160) tapA 2515382..2516143 (-) 762 WP_029726724.1 amyloid fiber anchoring/assembly protein TapA -
  PIB33_RS13165 (PIB33_13165) yqzG 2516415..2516741 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  PIB33_RS13170 (PIB33_13170) spoIITA 2516783..2516962 (-) 180 WP_029726723.1 YqzE family protein -
  PIB33_RS13175 (PIB33_13175) comGG 2517034..2517408 (-) 375 WP_029726722.1 ComG operon protein ComGG Machinery gene
  PIB33_RS13180 (PIB33_13180) comGF 2517409..2517792 (-) 384 WP_032722118.1 ComG operon protein ComGF Machinery gene
  PIB33_RS13185 (PIB33_13185) comGE 2517818..2518165 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  PIB33_RS13190 (PIB33_13190) comGD 2518149..2518580 (-) 432 WP_029726720.1 comG operon protein ComGD Machinery gene
  PIB33_RS13195 (PIB33_13195) comGC 2518570..2518866 (-) 297 WP_029726719.1 comG operon protein ComGC Machinery gene
  PIB33_RS13200 (PIB33_13200) comGB 2518880..2519917 (-) 1038 WP_029317912.1 comG operon protein ComGB Machinery gene
  PIB33_RS13205 (PIB33_13205) comGA 2519904..2520974 (-) 1071 WP_004399124.1 competence protein ComGA Machinery gene
  PIB33_RS13210 (PIB33_13210) - 2521187..2521384 (-) 198 WP_014480259.1 CBS domain-containing protein -
  PIB33_RS13215 (PIB33_13215) corA 2521386..2522339 (-) 954 WP_004399136.1 magnesium transporter CorA -
  PIB33_RS13220 (PIB33_13220) yqhB 2522482..2523810 (+) 1329 WP_003230150.1 hemolysin family protein -
  PIB33_RS13225 (PIB33_13225) rsbRD 2523863..2524699 (-) 837 WP_003230149.1 RsbT co-antagonist protein RsbRD -
  PIB33_RS13235 (PIB33_13235) mgsR 2524923..2525303 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  PIB33_RS13240 (PIB33_13240) yqgY 2525535..2525780 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40458.64 Da        Isoelectric Point: 9.0175

>NTDB_id=777029 PIB33_RS13205 WP_004399124.1 2519904..2520974(-) (comGA) [Bacillus subtilis strain GXD-20]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIEQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=777029 PIB33_RS13205 WP_004399124.1 2519904..2520974(-) (comGA) [Bacillus subtilis strain GXD-20]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATTGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGGCTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTTTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCGCAGGTCTGAAAGCAATTTTGCGCCATGACCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATTGAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGATTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGTCGCCAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTTTACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGGAAAGGAATTGCGCTCGGCTATTTAACAACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A809FNW6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

100

100

1