Detailed information NTG0776420

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: PI860_RS06000
• Genome accession: NZ_CP116258
• Coordinates: 1238632..1239507 (+)
• Length: 291 a.a.
• NCBI ID: WP_005314162.1
• Uniprot ID: A2T195
• Organism: Aeromonas salmonicida subsp. salmonicida strain J225
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1233632..1244507

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PI860_RS05980 (PI860_05985)	nadC	1233869..1234726 (+)	858	WP_005314155.1	carboxylating nicotinate-nucleotide diphosphorylase	-
PI860_RS05985 (PI860_05990)	tapA	1235061..1235489 (+)	429	WP_005314158.1	type IVa pilus major pilin TapA	-
PI860_RS05990 (PI860_05995)	pilB	1235493..1237199 (+)	1707	WP_005314160.1	PilB family type IVa pilus assembly ATPase TapB	Machinery gene
PI860_RS05995 (PI860_06000)	-	1237580..1238568 (+)	989	Protein_1148	type II secretion system F family protein	-
PI860_RS06000 (PI860_06005)	pilD	1238632..1239507 (+)	876	WP_005314162.1	prepilin peptidase	Machinery gene
PI860_RS06005 (PI860_06010)	coaE	1239527..1240141 (+)	615	WP_005314164.1	dephospho-CoA kinase	-
PI860_RS06010 (PI860_06015)	zapD	1240179..1240901 (+)	723	WP_005314166.1	cell division protein ZapD	-
PI860_RS06015 (PI860_06020)	yacG	1240911..1241105 (+)	195	WP_005314167.1	DNA gyrase inhibitor YacG	-
PI860_RS06020 (PI860_06025)	mutT	1241182..1241589 (-)	408	WP_005314168.1	8-oxo-dGTP diphosphatase MutT	-
PI860_RS06025 (PI860_06030)	-	1241598..1242770 (-)	1173	WP_005314169.1	tetratricopeptide repeat protein	-

Sequence

Protein

• Length: 291 a.a.
• Molecular weight: 32500.78 Da
• Isoelectric Point: 6.9383

>NTDB_id=776420 PI860_RS06000 WP_005314162.1 1238632..1239507(+) (pilD) [Aeromonas salmonicida subsp. salmonicida strain J225]
MTLLLELAHGLPWLYFSLVFLFSLMIGSFLNVVIHRLPIMLEREWQAEYRSYFSSDTPQPEDDERYNLMVPRSCCPRCNH
PITALENIPLLSWLWLKGRCRGCQAAISARYPLVELLTALLSVVVAMTLTPGWGTLAALLLTWVLVALTFIDLDKMLLPD
QLTLPLLWGGLLFNLLGGYVPLGDAVIGAMAGYLVLWSLYWAFKLLTGKEGMGYGDFKLLAALGAWLGWQALPIVLLLSS
LVGAIFGIGLILLRNHHQSKPIPFGPYLAIAGWIALLWGDSITRWYLSTIL

Nucleotide

• Length: 876 bp

>NTDB_id=776420 PI860_RS06000 WP_005314162.1 1238632..1239507(+) (pilD) [Aeromonas salmonicida subsp. salmonicida strain J225]
ATGACACTTCTTCTCGAGCTGGCACACGGCTTGCCATGGCTCTACTTCTCGCTGGTTTTTCTCTTCTCCCTGATGATCGG
CAGCTTTCTCAACGTGGTGATCCACCGCCTGCCCATCATGCTGGAACGGGAGTGGCAGGCCGAATATCGCAGCTATTTCA
GTTCAGACACGCCGCAGCCAGAAGATGACGAGCGCTACAATCTGATGGTGCCGCGCTCCTGCTGCCCTCGCTGCAATCAC
CCCATAACGGCCTTGGAAAACATTCCCCTGCTGAGCTGGCTCTGGCTCAAAGGTCGCTGCCGGGGCTGCCAGGCCGCCAT
TTCGGCTCGCTATCCGCTGGTGGAGCTGCTGACCGCGCTGCTCTCTGTGGTGGTTGCCATGACGCTCACCCCTGGCTGGG
GTACGCTGGCTGCCCTCCTGCTGACCTGGGTGCTGGTGGCGCTCACCTTTATCGATCTGGACAAGATGCTGCTGCCGGAC
CAGCTCACCTTGCCGCTACTGTGGGGCGGGCTGCTGTTCAACCTGCTGGGCGGATACGTGCCTCTCGGCGATGCTGTCAT
CGGCGCCATGGCAGGCTATCTGGTGCTGTGGAGCCTCTACTGGGCCTTCAAGCTGCTTACCGGCAAGGAGGGCATGGGCT
ATGGGGACTTCAAACTGCTGGCAGCGCTGGGAGCCTGGCTTGGCTGGCAGGCGCTGCCCATAGTACTGCTGCTCTCTTCT
CTGGTCGGCGCCATCTTCGGCATTGGCCTCATTTTGCTGCGCAACCATCACCAGAGCAAACCCATCCCCTTTGGCCCCTA
TCTCGCCATCGCAGGCTGGATCGCACTGCTCTGGGGCGATAGCATCACCCGCTGGTATCTCTCCACTATCCTCTGA

Domains

Predicted by InterproScan.

• Type IV leader peptidase family (Peptidase_A24) (140-248)

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (21-128)

3D structure

• AlphaFold DB: A2T195

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	60.764	98.969	0.601
pilD	Vibrio campbellii strain DS40M4	55.556	98.969	0.55
pilD	Acinetobacter baumannii D1279779	55.39	92.44	0.512
pilD	Acinetobacter nosocomialis M2	55.39	92.44	0.512
pilD	Neisseria gonorrhoeae MS11	50.746	92.096	0.467