Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PI860_RS05990 Genome accession   NZ_CP116258
Coordinates   1235493..1237199 (+) Length   568 a.a.
NCBI ID   WP_005314160.1    Uniprot ID   A2T193
Organism   Aeromonas salmonicida subsp. salmonicida strain J225     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1230493..1242199
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PI860_RS05965 (PI860_05970) pdhR 1231631..1232395 (-) 765 WP_005314150.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  PI860_RS05970 (PI860_05975) ampD 1232672..1233244 (-) 573 WP_005314151.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PI860_RS05975 (PI860_05980) - 1233394..1233864 (+) 471 WP_011898292.1 retropepsin-like aspartic protease family protein -
  PI860_RS05980 (PI860_05985) nadC 1233869..1234726 (+) 858 WP_005314155.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PI860_RS05985 (PI860_05990) tapA 1235061..1235489 (+) 429 WP_005314158.1 type IVa pilus major pilin TapA -
  PI860_RS05990 (PI860_05995) pilB 1235493..1237199 (+) 1707 WP_005314160.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  PI860_RS05995 (PI860_06000) - 1237580..1238568 (+) 989 Protein_1148 type II secretion system F family protein -
  PI860_RS06000 (PI860_06005) pilD 1238632..1239507 (+) 876 WP_005314162.1 prepilin peptidase Machinery gene
  PI860_RS06005 (PI860_06010) coaE 1239527..1240141 (+) 615 WP_005314164.1 dephospho-CoA kinase -
  PI860_RS06010 (PI860_06015) zapD 1240179..1240901 (+) 723 WP_005314166.1 cell division protein ZapD -
  PI860_RS06015 (PI860_06020) yacG 1240911..1241105 (+) 195 WP_005314167.1 DNA gyrase inhibitor YacG -
  PI860_RS06020 (PI860_06025) mutT 1241182..1241589 (-) 408 WP_005314168.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62577.06 Da        Isoelectric Point: 5.4384

>NTDB_id=776419 PI860_RS05990 WP_005314160.1 1235493..1237199(+) (pilB) [Aeromonas salmonicida subsp. salmonicida strain J225]
MTSSPNSGLALSLAASSLLSESDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLMESEQDALGMEEIDESEI
SELEVSDENSRLDESVNTADDDAPIVKYINKIMMDAIKRGASDLHFEPYETKYRIRFRIDGILHEIATPPVNLANRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNITEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
QQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRVSGLEKARLGVTSLA
EINRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=776419 PI860_RS05990 WP_005314160.1 1235493..1237199(+) (pilB) [Aeromonas salmonicida subsp. salmonicida strain J225]
ATGACCTCTAGCCCCAATAGCGGCCTGGCGTTAAGCCTGGCCGCCTCATCCCTGCTCAGTGAGTCCGACTCACAGCGTTA
CCTGAGCCAAGCCAAGGCCCAGCGCAAACCCTTCGTGACATTTCTGATTGAAAACGAGATCCTCGACAGCAAGGCGCTGG
CGGATTTTTGCGAGCTGGAATATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACCTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAAAAACACCATGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCAGA
CCCGACCAACGTGTCGGCACTGGAGGATTTTGGCTTCAGCTTCGGGCTCCATACCGAAGCCCTGCTGGTGGAAGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGATGGAAAGCGAGCAGGATGCCCTCGGCATGGAGGAGATCGACGAGTCGGAGATC
TCCGAGCTGGAAGTCTCGGACGAAAACTCCCGCCTCGATGAGAGCGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
ATACATCAATAAAATCATGATGGATGCCATCAAGCGCGGCGCCTCGGATCTGCACTTCGAACCCTATGAGACCAAGTACC
GTATCCGCTTTCGTATAGACGGCATACTGCACGAAATAGCGACGCCGCCGGTCAATCTGGCCAACCGCTTTTCGGCCCGC
CTCAAGGTGATGGCGCGACTCGATATCGCAGAGCGACGGCTGCCCCAGGATGGTCGCATCAAGTTAAAGCTGTCGCGCAA
CAAATCCATGGATATGCGGGTCAACACACTGCCCACCATGTGGGGCGAGAAGATCGTTATCCGTCTGCTGGACTCTTCCG
CAGCGCGGCTCAATATCGAACAGCTCGGCTTCGACGACCGGCAGAAGGCACAGTATCTGCGTGCCCTCTCCAAGCCCCAG
GGCATGATACTGGTGACGGGCCCGACCGGCTCTGGCAAGACGGTCTCTCTCTATACCGGCCTCAATATTCTCAACATCAC
AGAGGTGAACATCTCAACCGCCGAAGATCCGGTGGAGATCAACCTGCCAGGCGTCAACCAGGTACAAGTCAATCCCAAGG
CGGGGTTGACCTTCGCCAGCGCCCTGCGATCCTTTCTTCGCCAGGATCCCGACGTTGTGATGGTGGGGGAAATCCGGGAT
CTGGAAACCGCCGAGATTGCCATCAAAGCCGCCCAGACCGGCCACCTGGTGCTCTCCACCCTGCACACCAACTCGGCAGC
CGAAACCCTGACCCGGATGATGAACATGGGGGTCCCGGCCTTCAATATCGCCTCTTCGGTGACCCTCATCATGGCCCAGC
GCCTCGCTCGCAAGCTCTGCGATCACTGCAAGGCACCGGAAGTGGTGCCCGAAGCTGAATTGCTGGAGCTGGGCTTCACC
CAGCAGCAGTTGGCCGCCGGCTTGCGCCTGTTCAAGCCAGTAGGCTGCAAAGAGTGCTCGGGGGGCTACAAGGGTCGGGT
CGGTATCTACGAAATCATGCTAATGTCTGAAAACATTGCAAAATTGATTATGCAAGGAGCCAACTCCCTGCAAATTGCAG
CGATTGCTCAAAAAGAGGGGATGCGAACCCTGCGTGTCTCGGGCTTGGAGAAGGCCCGCCTGGGGGTGACCAGCCTGGCA
GAGATCAACCGGGTGACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A2T193

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.439

100

0.556
  pilB Acinetobacter baumannii D1279779

55.379

99.824

0.553
  pilB Legionella pneumophila strain ERS1305867

54.24

99.648

0.54
  pilB Vibrio cholerae strain A1552

54.336

99.472

0.54
  pilB Vibrio campbellii strain DS40M4

52.12

99.648

0.519
  pilB Vibrio parahaemolyticus RIMD 2210633

51.423

98.944

0.509
  pilF Neisseria gonorrhoeae MS11

46.277

99.296

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

38.879

97.359

0.379
  pilF Thermus thermophilus HB27

40.152

92.958

0.373