Detailed information NTG0775277

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: PF996_RS13735
• Genome accession: NZ_CP116014
• Coordinates: 2688492..2689106 (-)
• Length: 204 a.a.
• NCBI ID: WP_017418180.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain SRCM124633
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2683492..2694106

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF996_RS13705 (PF996_13705)	rpsT	2683792..2684058 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PF996_RS13710 (PF996_13710)	holA	2684075..2685116 (-)	1042	Protein_2656	DNA polymerase III subunit delta	-
PF996_RS13715 (PF996_13715)	-	2685156..2685310 (-)	155	Protein_2657	hypothetical protein	-
PF996_RS13720 (PF996_13720)	-	2685351..2685485 (+)	135	WP_003152870.1	YqzM family protein	-
PF996_RS13725 (PF996_13725)	comEC	2685504..2687855 (-)	2352	WP_271265649.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PF996_RS13730 (PF996_13730)	-	2687856..2688425 (-)	570	WP_003152868.1	ComE operon protein 2	-
PF996_RS13735 (PF996_13735)	comEA	2688492..2689106 (-)	615	WP_017418180.1	helix-hairpin-helix domain-containing protein	Machinery gene
PF996_RS13740 (PF996_13740)	comER	2689165..2689986 (+)	822	WP_014305449.1	late competence protein ComER	-
PF996_RS13745 (PF996_13745)	-	2690055..2690792 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
PF996_RS13750 (PF996_13750)	rsfS	2690789..2691145 (-)	357	WP_003152864.1	ribosome silencing factor	-
PF996_RS13755 (PF996_13755)	yqeK	2691142..2691723 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PF996_RS13760 (PF996_13760)	-	2691713..2692282 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
PF996_RS13765 (PF996_13765)	yhbY	2692293..2692583 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PF996_RS13770 (PF996_13770)	aroE	2692577..2693419 (-)	843	WP_003152856.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22218.33 Da
• Isoelectric Point: 5.4841

>NTDB_id=775277 PF996_RS13735 WP_017418180.1 2688492..2689106(-) (comEA) [Bacillus velezensis strain SRCM124633]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAVGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=775277 PF996_RS13735 WP_017418180.1 2688492..2689106(-) (comEA) [Bacillus velezensis strain SRCM124633]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGCGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGTGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• Helix-hairpin-helix motif (HHH_3) (141-202)

• SLBB domain (SLBB) (68-122)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363