NTDB

Overview

Name	comEA	Type	Machinery gene
Locus tag	PF986_RS07720	Genome accession	NZ_CP116013
Coordinates	1651209..1651823 (-)	Length	204 a.a.
NCBI ID	WP_260654647.1	Uniprot ID	-
Organism	Bacillus velezensis strain SRCM124349
Function	dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1646209..1656823

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PF986_RS07690 (PF986_07690)	rpsT	1646512..1646778 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
PF986_RS07695 (PF986_07695)	holA	1646795..1647836 (-)	1042	Protein_1533	DNA polymerase III subunit delta	-
PF986_RS07700 (PF986_07700)	-	1647876..1648027 (-)	152	Protein_1534	hypothetical protein	-
PF986_RS07705 (PF986_07705)	-	1648068..1648202 (+)	135	WP_003152870.1	YqzM family protein	-
PF986_RS07710 (PF986_07710)	comEC	1648221..1650572 (-)	2352	WP_025649830.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
PF986_RS07715 (PF986_07715)	-	1650573..1651142 (-)	570	WP_003152868.1	ComE operon protein 2	-
PF986_RS07720 (PF986_07720)	comEA	1651209..1651823 (-)	615	WP_260654647.1	helix-hairpin-helix domain-containing protein	Machinery gene
PF986_RS07725 (PF986_07725)	comER	1651882..1652703 (+)	822	WP_014305449.1	late competence protein ComER	-
PF986_RS07730 (PF986_07730)	-	1652772..1653509 (-)	738	WP_014305450.1	class I SAM-dependent DNA methyltransferase	-
PF986_RS07735 (PF986_07735)	rsfS	1653506..1653862 (-)	357	WP_003152864.1	ribosome silencing factor	-
PF986_RS07740 (PF986_07740)	yqeK	1653859..1654440 (-)	582	WP_017418181.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
PF986_RS07745 (PF986_07745)	-	1654430..1654999 (-)	570	WP_017418182.1	nicotinate-nucleotide adenylyltransferase	-
PF986_RS07750 (PF986_07750)	yhbY	1655010..1655300 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
PF986_RS07755 (PF986_07755)	aroE	1655294..1656136 (-)	843	WP_260654646.1	shikimate dehydrogenase	-

Sequence

Protein

Download Length: 204 a.a. Molecular weight: 22147.25 Da Isoelectric Point: 5.1985

                                >NTDB_id=775188 PF986_RS07720 WP_260654647.1 1651209..1651823(-) (comEA) [Bacillus velezensis strain SRCM124349]

MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY

EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG

QKKAEAIIAYREENGLFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

Download Length: 615 bp

                                >NTDB_id=775188 PF986_RS07720 WP_260654647.1 1651209..1651823(-) (comEA) [Bacillus velezensis strain SRCM124349]

ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT

TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT

CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT

GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT

TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG

CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG

CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACTGTTTCAGACTGCTGAAGATTTGATGAATGTATC

AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.333	94.118	0.549
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363

Detailed information NTG0775188