Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PF975_RS07860 Genome accession   NZ_CP116010
Coordinates   1541853..1542923 (+) Length   356 a.a.
NCBI ID   WP_014418378.1    Uniprot ID   A0AAP4DGV9
Organism   Bacillus velezensis strain SRCM123815     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1536853..1547923
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PF975_RS07830 (PF975_07830) - 1537326..1537571 (-) 246 WP_003153078.1 DUF2626 domain-containing protein -
  PF975_RS07835 (PF975_07835) - 1537808..1538188 (+) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PF975_RS07845 (PF975_07845) - 1538395..1539225 (+) 831 WP_025649847.1 STAS domain-containing protein -
  PF975_RS07850 (PF975_07850) - 1539263..1540564 (-) 1302 WP_029973873.1 hemolysin family protein -
  PF975_RS07855 (PF975_07855) - 1540710..1541660 (+) 951 WP_014418379.1 magnesium transporter CorA family protein -
  PF975_RS07860 (PF975_07860) comGA 1541853..1542923 (+) 1071 WP_014418378.1 competence type IV pilus ATPase ComGA Machinery gene
  PF975_RS07865 (PF975_07865) comGB 1542910..1543947 (+) 1038 WP_053284924.1 competence type IV pilus assembly protein ComGB Machinery gene
  PF975_RS07870 (PF975_07870) comGC 1543994..1544260 (+) 267 WP_050496152.1 competence type IV pilus major pilin ComGC Machinery gene
  PF975_RS07875 (PF975_07875) comGD 1544250..1544687 (+) 438 WP_088056451.1 competence type IV pilus minor pilin ComGD Machinery gene
  PF975_RS07880 (PF975_07880) comGE 1544671..1544985 (+) 315 WP_088056452.1 competence type IV pilus minor pilin ComGE -
  PF975_RS07885 (PF975_07885) comGF 1544999..1545394 (+) 396 WP_088056453.1 competence type IV pilus minor pilin ComGF -
  PF975_RS07890 (PF975_07890) comGG 1545395..1545772 (+) 378 WP_053284923.1 competence type IV pilus minor pilin ComGG Machinery gene
  PF975_RS07895 (PF975_07895) - 1545829..1546008 (+) 180 WP_003153093.1 YqzE family protein -
  PF975_RS07900 (PF975_07900) - 1546049..1546378 (-) 330 WP_012117979.1 DUF3889 domain-containing protein -
  PF975_RS07905 (PF975_07905) tapA 1546637..1547308 (+) 672 WP_053284922.1 amyloid fiber anchoring/assembly protein TapA -
  PF975_RS07910 (PF975_07910) sipW 1547280..1547864 (+) 585 WP_012117977.1 signal peptidase I SipW -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40529.81 Da        Isoelectric Point: 9.3485

>NTDB_id=774955 PF975_RS07860 WP_014418378.1 1541853..1542923(+) (comGA) [Bacillus velezensis strain SRCM123815]
MKNIETYSRNIINEAYTARASDIHIVPRERDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKQQIPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=774955 PF975_RS07860 WP_014418378.1 1541853..1542923(+) (comGA) [Bacillus velezensis strain SRCM123815]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAGGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGTG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGAAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACAGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCAGCAGATTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCAGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCGCACGGCCTGCTTCTGTTTACCGGCCCGACCGGATCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGCTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGGGATGCCGAGACGGCTGAAATCGCAGTCCGCGCGGCAATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGCGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACACGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACGACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

82.303

100

0.823
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.892

98.596

0.374