Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   PG915_RS02325 Genome accession   NZ_CP115920
Coordinates   514485..515069 (+) Length   194 a.a.
NCBI ID   WP_353497716.1    Uniprot ID   A0AAU8BJ92
Organism   Vibrio chaetopteri strain CB1-14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 509485..520069
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG915_RS02310 (PG915_02305) - 510121..512769 (-) 2649 WP_353497713.1 penicillin-binding protein 1A -
  PG915_RS02315 (PG915_02310) pilM 512916..513893 (+) 978 WP_353497714.1 pilus assembly protein PilM -
  PG915_RS02320 (PG915_02315) - 513895..514485 (+) 591 WP_353497715.1 PilN domain-containing protein -
  PG915_RS02325 (PG915_02320) pilO 514485..515069 (+) 585 WP_353497716.1 type 4a pilus biogenesis protein PilO Machinery gene
  PG915_RS02330 (PG915_02325) - 515059..515565 (+) 507 WP_353497717.1 pilus assembly protein PilP -
  PG915_RS02335 (PG915_02330) pilQ 515565..517301 (+) 1737 WP_353498645.1 type IV pilus secretin PilQ family protein Machinery gene
  PG915_RS02340 (PG915_02335) aroK 517487..518005 (+) 519 WP_353497718.1 shikimate kinase AroK -
  PG915_RS02345 (PG915_02340) aroB 518036..519121 (+) 1086 WP_353497719.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 194 a.a.        Molecular weight: 22073.32 Da        Isoelectric Point: 4.6482

>NTDB_id=774438 PG915_RS02325 WP_353497716.1 514485..515069(+) (pilO) [Vibrio chaetopteri strain CB1-14]
MSLDLELDELSSWPLIPQLVVVGIVFVMAALVSGWLSVKPSLEDLDYYKQQETQLKQELQVVARKAAMLPVMSAHLSDLS
DHYDRLLRQLPAQQELASLLADINEQGLNNHLTFTRINWGKRQPQKFLLKLPLDIELTGSYDDIGRYAQAIAELPRIVLI
ENAEWQRVSLESSTLHFRVNATTYQFIAEAEGEK

Nucleotide


Download         Length: 585 bp        

>NTDB_id=774438 PG915_RS02325 WP_353497716.1 514485..515069(+) (pilO) [Vibrio chaetopteri strain CB1-14]
ATGTCACTCGACCTTGAACTTGATGAATTGAGCAGTTGGCCGTTGATTCCACAGCTTGTCGTGGTCGGTATTGTGTTTGT
GATGGCGGCACTGGTCAGTGGATGGCTTTCGGTGAAGCCCAGCCTTGAAGACCTCGATTACTACAAACAACAAGAAACTC
AACTGAAGCAAGAGCTGCAGGTTGTCGCTCGCAAGGCCGCTATGTTGCCTGTGATGTCAGCGCATCTTAGCGATCTTTCT
GACCATTACGACAGGCTTTTGCGTCAATTGCCTGCTCAGCAAGAGCTAGCAAGCTTGCTCGCAGACATCAACGAGCAAGG
CCTCAACAATCATCTTACCTTCACTCGTATCAATTGGGGTAAGCGTCAGCCGCAGAAGTTTTTATTAAAGCTTCCTTTAG
ATATCGAGCTGACCGGAAGTTATGACGATATCGGCCGCTACGCTCAGGCAATTGCTGAGCTGCCTCGCATTGTACTGATC
GAAAATGCTGAATGGCAGAGAGTCAGTTTGGAAAGCAGCACTCTGCACTTTCGTGTTAATGCCACTACCTACCAGTTTAT
TGCGGAGGCTGAAGGTGAAAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(46-187)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Vibrio cholerae strain A1552

53.403

98.454

0.526
  pilO Vibrio campbellii strain DS40M4

50.82

94.33

0.479