Detailed information NTG0773816

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: PAK23_RS11645
• Genome accession: NZ_CP115817
• Coordinates: 2499572..2500795 (-)
• Length: 407 a.a.
• NCBI ID: WP_264148502.1
• Uniprot ID: -
• Organism: Pseudomonas mendocina strain GD22SC3150TT
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2494572..2505795

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PAK23_RS11630	-	2495718..2496617 (+)	900	WP_276486702.1	hydroxymethylglutaryl-CoA lyase	-
PAK23_RS11635	-	2496756..2498711 (+)	1956	WP_276486703.1	acetoacetate--CoA ligase	-
PAK23_RS11640	-	2498796..2499575 (+)	780	WP_276486704.1	ABC transporter substrate-binding protein	-
PAK23_RS11645	pilU	2499572..2500795 (-)	1224	WP_264148502.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
PAK23_RS11650	-	2500944..2501222 (+)	279	WP_233787615.1	peptidylprolyl isomerase	-
PAK23_RS11655	-	2501228..2502139 (-)	912	WP_264148501.1	LysR family transcriptional regulator	-
PAK23_RS11660	-	2502209..2502688 (-)	480	WP_264148500.1	nuclear transport factor 2 family protein	-
PAK23_RS11665	-	2502698..2503288 (-)	591	WP_264148499.1	TetR/AcrR family transcriptional regulator	-
PAK23_RS11670	-	2503441..2504364 (-)	924	WP_276486705.1	LysR family transcriptional regulator	-
PAK23_RS11675	-	2504504..2504899 (+)	396	WP_276486706.1	RidA family protein	-
PAK23_RS11680	nfsB	2504946..2505596 (-)	651	WP_276486707.1	oxygen-insensitive NAD(P)H nitroreductase	-

Sequence

Protein

• Length: 407 a.a.
• Molecular weight: 44808.22 Da
• Isoelectric Point: 6.0784

>NTDB_id=773816 PAK23_RS11645 WP_264148502.1 2499572..2500795(-) (pilU) [Pseudomonas mendocina strain GD22SC3150TT]
MDLHAMLKILSSQDGSDLYLSTGAPPCAKFNGVLKALSQEPMKAGDVAVIADSIMDSAQREEFERELEMNLAISVTGVGR
FRINIFKQRNEVSIVARNIKLDIPRFEDLKLPEVLLSTVMEKRGLVLFVGGTGSGKSTSLAALIDYRNRNSGGHIITIED
PVEYVHRHKKSIINQREVGVDTRSFHAALKNTLRQAPDVILIGEIRDRETMEHALAFADTGHLAISTLHANNANQALDRI
INFFPEDRRPQLLNDLGNNLKAFVSQRLVKTVDGKRRAAVEVMLGTPTVRDLIKRNEFSELKEIMEKSKNLGMQTFDQAL
IDLVHEGAIDEEEAVKNADSANNVRLKLKLYRDNPANAKPAPPVPAAAPATPVAAPKAAAAGDWGLELKLEDIEEELPPE
DPGRQGI

Nucleotide

• Length: 1224 bp

>NTDB_id=773816 PAK23_RS11645 WP_264148502.1 2499572..2500795(-) (pilU) [Pseudomonas mendocina strain GD22SC3150TT]
ATGGATCTCCACGCAATGCTGAAAATCCTGTCCAGTCAGGATGGCTCCGACCTCTACCTCTCCACCGGCGCGCCGCCCTG
TGCCAAGTTCAACGGCGTGCTCAAGGCGCTGAGCCAGGAGCCGATGAAAGCCGGCGACGTCGCGGTCATTGCCGATTCGA
TCATGGATAGCGCGCAGCGCGAGGAATTCGAACGCGAGCTGGAGATGAACCTGGCGATTTCCGTGACCGGCGTCGGCCGC
TTTCGTATCAACATCTTCAAGCAGCGCAACGAGGTATCCATCGTCGCGCGCAACATCAAGCTGGACATTCCCCGTTTCGA
AGACCTGAAGCTACCTGAAGTGCTCCTAAGCACTGTGATGGAAAAGCGTGGCCTCGTACTGTTCGTCGGGGGCACCGGCT
CGGGCAAGTCGACGTCCCTGGCGGCGCTGATCGACTACCGCAACCGCAACAGCGGTGGCCATATCATCACCATCGAAGAC
CCGGTGGAGTACGTGCACCGGCACAAGAAGTCGATCATCAACCAGCGCGAAGTGGGGGTGGACACACGCAGCTTCCACGC
CGCGCTGAAGAACACCCTGCGTCAGGCGCCGGACGTGATCCTCATCGGCGAGATCCGCGACCGCGAAACCATGGAGCATG
CGCTGGCGTTCGCCGATACCGGGCACCTGGCGATCTCCACCCTGCATGCCAACAATGCCAACCAGGCGCTGGACCGCATC
ATCAACTTCTTCCCCGAGGATCGTCGCCCGCAACTGCTCAATGACCTGGGCAACAACCTCAAGGCGTTCGTTTCGCAGCG
TCTGGTCAAGACCGTCGATGGCAAGCGTCGCGCCGCGGTGGAGGTGATGCTGGGCACGCCGACGGTGCGCGATCTGATCA
AGCGCAACGAGTTTTCCGAGCTCAAGGAAATCATGGAGAAATCCAAGAACCTCGGCATGCAGACCTTCGATCAGGCCCTG
ATCGACCTGGTGCACGAGGGCGCCATTGACGAAGAGGAGGCGGTGAAGAACGCCGATTCGGCGAACAACGTGCGTCTCAA
GCTCAAGCTCTATCGCGACAACCCGGCCAATGCCAAACCGGCACCCCCTGTGCCTGCCGCTGCGCCCGCCACGCCGGTCG
CCGCTCCGAAAGCGGCTGCTGCTGGCGATTGGGGGCTGGAGCTGAAGCTGGAGGATATCGAAGAAGAGCTGCCGCCAGAG
GATCCAGGGCGTCAGGGAATCTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (111-248)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	56.41	86.241	0.486
pilU	Acinetobacter baylyi ADP1	51.989	86.486	0.45
pilU	Vibrio cholerae strain A1552	51.558	86.732	0.447
pilT	Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539	38.677	96.56	0.373