Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   PAN99_RS11935 Genome accession   NZ_CP115604
Coordinates   2469973..2471043 (-) Length   356 a.a.
NCBI ID   WP_044053465.1    Uniprot ID   -
Organism   Bacillus velezensis strain FJAT-54560     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2464973..2476043
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PAN99_RS11885 (PAN99_11885) sipW 2465033..2465617 (-) 585 WP_012117977.1 signal peptidase I SipW -
  PAN99_RS11890 (PAN99_11890) tapA 2465589..2466260 (-) 672 WP_003153099.1 amyloid fiber anchoring/assembly protein TapA -
  PAN99_RS11895 (PAN99_11895) - 2466519..2466848 (+) 330 WP_003153097.1 DUF3889 domain-containing protein -
  PAN99_RS11900 (PAN99_11900) - 2466888..2467067 (-) 180 WP_003153093.1 YqzE family protein -
  PAN99_RS11905 (PAN99_11905) comGG 2467124..2467501 (-) 378 WP_014305410.1 competence type IV pilus minor pilin ComGG Machinery gene
  PAN99_RS11910 (PAN99_11910) comGF 2467502..2468002 (-) 501 WP_235570156.1 competence type IV pilus minor pilin ComGF -
  PAN99_RS11915 (PAN99_11915) comGE 2467911..2468225 (-) 315 WP_057080485.1 competence type IV pilus minor pilin ComGE Machinery gene
  PAN99_RS11920 (PAN99_11920) comGD 2468209..2468646 (-) 438 WP_015388002.1 competence type IV pilus minor pilin ComGD Machinery gene
  PAN99_RS11925 (PAN99_11925) comGC 2468636..2468944 (-) 309 WP_003153087.1 competence type IV pilus major pilin ComGC Machinery gene
  PAN99_RS11930 (PAN99_11930) comGB 2468949..2469986 (-) 1038 WP_063174751.1 competence type IV pilus assembly protein ComGB Machinery gene
  PAN99_RS11935 (PAN99_11935) comGA 2469973..2471043 (-) 1071 WP_044053465.1 competence type IV pilus ATPase ComGA Machinery gene
  PAN99_RS11940 (PAN99_11940) - 2471235..2472185 (-) 951 WP_014305415.1 magnesium transporter CorA family protein -
  PAN99_RS11945 (PAN99_11945) - 2472331..2473632 (+) 1302 WP_014305416.1 hemolysin family protein -
  PAN99_RS11950 (PAN99_11950) - 2473670..2474500 (-) 831 WP_044053466.1 STAS domain-containing protein -
  PAN99_RS11960 (PAN99_11960) - 2474706..2475086 (-) 381 WP_003153079.1 Spx/MgsR family RNA polymerase-binding regulatory protein -
  PAN99_RS11965 (PAN99_11965) - 2475323..2475568 (+) 246 WP_003153078.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40515.82 Da        Isoelectric Point: 9.4729

>NTDB_id=772733 PAN99_RS11935 WP_044053465.1 2469973..2471043(-) (comGA) [Bacillus velezensis strain FJAT-54560]
MKNIETYSRNIINEAYTARASDIHIVPREKDAFIHFRIGHALVKKRVLKKEECVRLISHFKFLSAMDIGERRKPQNGSLS
LPLPTETVHLRMSTLPTMNDESLVIRLLPKRQVPPLDKLSLFRGAGAALLSFLKHSHGLLLFTGPTGSGKTTTLYSLVNY
AKRHFNRRIVTLEDPVETRDEDVLQVQVNEKAGVTYSAGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLSSLH
TRDAKGAIYRLLEFGVNMTEIEQTVIAIAAQRLVDLTCPFCKEENCSVYCRMYRQTRRAGIYELLYGKNLTQCFQEAKGE
HANFQYQTLRRLIRKGIALGYVTTDNYDRWVYHEAD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=772733 PAN99_RS11935 WP_044053465.1 2469973..2471043(-) (comGA) [Bacillus velezensis strain FJAT-54560]
GTGAAAAATATTGAAACATACAGCAGGAACATCATAAACGAGGCCTATACGGCAAGGGCTTCAGATATACACATTGTGCC
GAGAGAAAAGGATGCTTTCATTCATTTTCGCATCGGCCATGCTCTTGTGAAAAAAAGAGTCTTAAAAAAAGAGGAATGCG
TAAGGCTTATTTCTCATTTCAAGTTTTTATCGGCCATGGATATAGGAGAAAGACGTAAGCCGCAAAACGGGTCATTGTCT
CTGCCGCTTCCGACGGAAACCGTTCATTTGAGAATGTCAACCTTGCCGACAATGAATGATGAAAGTCTTGTCATCAGGCT
TTTGCCTAAGCGGCAGGTTCCCCCTCTGGATAAACTGTCCTTATTTCGGGGGGCGGGCGCCGCACTATTGTCCTTTCTGA
AGCATTCACACGGCCTGCTTCTGTTTACCGGCCCGACCGGCTCTGGAAAAACGACCACTCTTTACTCGCTTGTCAACTAT
GCAAAACGGCATTTCAACCGGCGGATTGTCACGCTTGAAGACCCCGTAGAAACAAGAGATGAGGATGTGTTGCAAGTTCA
GGTGAATGAAAAAGCCGGAGTCACATATTCCGCCGGTTTAAAAGCGATTTTAAGACATGATCCCGATATGATCATCTTAG
GGGAAATCAGAGATGCCGAAACGGCTGAAATCGCAGTCCGCGCGGCCATGACGGGACACCTCGTTCTTTCAAGCCTGCAT
ACAAGAGATGCTAAGGGCGCCATATACAGGCTGCTGGAGTTTGGGGTGAACATGACAGAAATTGAACAAACCGTCATTGC
CATTGCCGCTCAGCGTCTGGTTGATTTAACATGTCCGTTCTGCAAAGAGGAAAACTGTTCCGTGTACTGCCGGATGTACA
GGCAGACCCGCCGTGCGGGCATTTATGAACTGCTCTACGGGAAAAACCTCACCCAATGCTTTCAGGAAGCAAAGGGAGAG
CACGCCAATTTTCAATATCAGACGCTCCGCAGGCTGATCAGAAAAGGAATCGCGCTCGGCTATGTCACAACCGACAATTA
CGATCGGTGGGTGTATCATGAAGCGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

81.742

100

0.817
  pilB Glaesserella parasuis strain SC1401

38.793

97.753

0.379
  pilF Thermus thermophilus HB27

37.607

98.596

0.371