Detailed information NTG0772470

Bioinformatically predicted

Overview

• Name: comFA
• Type: Machinery gene
• Locus tag: PDI73_RS03430
• Genome accession: NZ_CP115479
• Coordinates: 712690..714012 (-)
• Length: 440 a.a.
• NCBI ID: WP_277730402.1
• Uniprot ID: -
• Organism: Lactococcus lactis strain EP2
• Function: ssDNA transport into the cell (predicted from homology) DNA binding and uptake

Genomic Context

Location: 707690..719012

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PDI73_RS03400 (PDI73_03400)	-	708833..709801 (+)	969	WP_003130649.1	PhoH family protein	-
PDI73_RS03405 (PDI73_03405)	-	709859..710338 (+)	480	WP_058206727.1	NUDIX hydrolase	-
PDI73_RS03410 (PDI73_03410)	ybeY	710490..710978 (+)	489	WP_021722516.1	rRNA maturation RNase YbeY	-
PDI73_RS03415 (PDI73_03415)	-	710962..711417 (+)	456	WP_003130653.1	diacylglycerol kinase	-
PDI73_RS03420 (PDI73_03420)	-	711431..712009 (+)	579	WP_021722515.1	nucleotidyltransferase family protein	-
PDI73_RS03425 (PDI73_03425)	-	712043..712693 (-)	651	WP_033899429.1	ComF family protein	-
PDI73_RS03430 (PDI73_03430)	comFA	712690..714012 (-)	1323	WP_277730402.1	DEAD/DEAH box helicase	Machinery gene
PDI73_RS03435 (PDI73_03435)	-	714069..714698 (+)	630	WP_003130660.1	YigZ family protein	-
PDI73_RS03440 (PDI73_03440)	-	714832..715329 (+)	498	WP_003130662.1	VanZ family protein	-
PDI73_RS03445 (PDI73_03445)	-	715377..716396 (+)	1020	WP_012897709.1	DUF475 domain-containing protein	-
PDI73_RS03450 (PDI73_03450)	-	716512..717258 (+)	747	WP_187398315.1	bifunctional metallophosphatase/5'-nucleotidase	-
PDI73_RS03455 (PDI73_03455)	glyQ	717544..718497 (+)	954	WP_003130667.1	glycine--tRNA ligase subunit alpha	-

Sequence

Protein

• Length: 440 a.a.
• Molecular weight: 50114.59 Da
• Isoelectric Point: 10.0010

>NTDB_id=772470 PDI73_RS03430 WP_277730402.1 712690..714012(-) (comFA) [Lactococcus lactis strain EP2]
MSTNQEKLFGRLLLKNDILQLIKSTDKISVSKIFSNFLLEAKVNPILGMTSISSNKIKCNRCGTVHIKNSVKLPIGVFYC
PSCIQLGRVRSDEFLYFLPQKNFPKKSYINWSGKLTENQKPISNALCQEINSHQQIIVQAVTGAGKTEMIYQVIEQILES
GGVVGLASPRIDVCLELHQRLSHDFSCKIPLLYHDGDSYFRAPLIIMTSHQLLRFKEAFDLLIIDEVDAFPFRDNEMLYF
AAEKARKIEGNLIYLTATSTDKLEKDIKKQKLYPLFLPSRFHNFPLVVPKFFWKNKFDKKLIEQRNSGFPLLIFAAEIEF
GQEFAKQLQLKFPKEKIASVASTTKDRLEIVKAFRNKEITILIATSILERGVTFPNVDVFVINSEHPNFTKSALIQMAGR
VGRSPERPTGLVSFFHYGKSKAMCQAVREIKKMNQLGGFS

Nucleotide

• Length: 1323 bp

>NTDB_id=772470 PDI73_RS03430 WP_277730402.1 712690..714012(-) (comFA) [Lactococcus lactis strain EP2]
ATGAGCACTAATCAAGAAAAGTTATTTGGCCGTTTATTATTAAAAAATGATATTTTACAACTTATAAAAAGTACTGACAA
GATTTCTGTCAGTAAAATTTTTAGTAATTTTTTGTTAGAAGCGAAGGTGAATCCAATTTTGGGAATGACTTCAATTTCTT
CCAATAAAATAAAATGCAACCGTTGTGGGACTGTTCATATAAAAAATTCTGTCAAACTTCCAATTGGTGTATTTTACTGT
CCAAGTTGTATTCAATTAGGTCGAGTCCGCTCCGATGAATTCTTGTACTTTCTGCCACAAAAGAATTTCCCAAAGAAATC
ATATATAAACTGGTCGGGAAAACTGACAGAGAATCAAAAACCAATTTCAAATGCCCTCTGTCAGGAAATTAATTCTCATC
AGCAAATAATTGTCCAAGCTGTGACTGGAGCTGGAAAAACTGAAATGATTTATCAAGTCATTGAACAAATTTTAGAAAGC
GGTGGGGTTGTTGGTCTAGCTAGTCCAAGAATTGATGTTTGTCTTGAACTTCATCAGCGATTATCACATGATTTTTCCTG
TAAGATTCCACTCTTATATCATGATGGCGACAGCTATTTCCGAGCTCCATTAATAATAATGACCAGTCATCAGCTTTTAC
GTTTCAAGGAAGCTTTTGATTTGCTGATTATTGATGAAGTTGATGCCTTTCCCTTTAGAGATAATGAAATGCTTTATTTT
GCGGCAGAAAAAGCAAGAAAAATAGAAGGAAATTTAATATATTTGACCGCAACTTCTACTGACAAACTTGAAAAAGATAT
AAAAAAGCAAAAACTCTATCCTTTGTTTCTCCCGAGTCGTTTTCACAATTTTCCTTTAGTCGTGCCTAAATTTTTTTGGA
AAAATAAATTTGATAAGAAATTAATTGAGCAAAGAAATAGTGGCTTTCCTCTTCTTATTTTTGCTGCTGAAATTGAATTT
GGACAAGAATTTGCAAAACAACTACAATTGAAATTTCCTAAAGAAAAAATTGCTTCCGTTGCTTCAACAACAAAAGATAG
ATTGGAAATTGTTAAAGCTTTTAGAAATAAAGAAATTACTATTTTAATAGCGACTTCAATTCTTGAACGGGGAGTCACTT
TTCCAAATGTTGATGTTTTTGTCATCAACAGTGAGCACCCAAACTTCACTAAATCCGCACTGATACAAATGGCTGGACGT
GTGGGTCGTAGTCCTGAACGTCCAACAGGCTTAGTTAGTTTTTTTCATTACGGAAAATCCAAAGCAATGTGTCAGGCAGT
TAGAGAAATCAAAAAAATGAACCAACTGGGAGGTTTTTCATGA

Domains

Predicted by InterproScan.

• Type III restriction enzyme, res subunit (ResIII) (114-232)

• Helicase conserved C-terminal domain (Helicase_C) (300-403)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comFA	Lactococcus lactis subsp. cremoris KW2	74.25	90.909	0.675
comFA/cflA	Streptococcus mitis SK321	51.759	90.455	0.468
comFA/cflA	Streptococcus pneumoniae Rx1	51.759	90.455	0.468
comFA/cflA	Streptococcus pneumoniae D39	51.759	90.455	0.468
comFA/cflA	Streptococcus pneumoniae R6	51.759	90.455	0.468
comFA/cflA	Streptococcus pneumoniae TIGR4	51.759	90.455	0.468
comFA/cflA	Streptococcus mitis NCTC 12261	51.005	90.455	0.461
comFA	Bacillus subtilis subsp. subtilis str. 168	37.251	100	0.382