NTDB

Overview

Name	comGF	Type	Machinery gene
Locus tag	PDI59_RS07540	Genome accession	NZ_CP115478
Coordinates	1519714..1520211 (-)	Length	165 a.a.
NCBI ID	WP_001788897.1	Uniprot ID	-
Organism	Staphylococcus aureus strain HP20814-062
Function	dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1514714..1525211

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
PDI59_RS07510 (PDI59_07515)	gcvPA	1514822..1516168 (-)	1347	WP_000019691.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
PDI59_RS07515 (PDI59_07520)	gcvT	1516188..1517237 (-)	1050	WP_190362429.1	glycine cleavage system aminomethyltransferase GcvT	-
PDI59_RS07520 (PDI59_07525)	-	1517219..1517293 (-)	75	Protein_1478	hypothetical protein	-
PDI59_RS07525 (PDI59_07530)	-	1517296..1518615 (-)	1320	Protein_1479	ISL3-like element IS1181 family transposase	-
PDI59_RS07530 (PDI59_07535)	-	1518958..1519481 (-)	524	Protein_1480	shikimate kinase	-
PDI59_RS07535 (PDI59_07540)	-	1519471..1519617 (-)	147	WP_001792109.1	hypothetical protein	-
PDI59_RS07540 (PDI59_07545)	comGF	1519714..1520211 (-)	498	WP_001788897.1	competence type IV pilus minor pilin ComGF	Machinery gene
PDI59_RS07545 (PDI59_07550)	comGE	1520129..1520428 (-)	300	WP_000844410.1	hypothetical protein	Machinery gene
PDI59_RS07550 (PDI59_07555)	comGD	1520415..1520861 (-)	447	WP_001788899.1	competence type IV pilus minor pilin ComGD	Machinery gene
PDI59_RS07555 (PDI59_07560)	comGC	1520839..1521150 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
PDI59_RS07560 (PDI59_07565)	comGB	1521164..1522234 (-)	1071	WP_000776422.1	competence type IV pilus assembly protein ComGB	Machinery gene
PDI59_RS07565 (PDI59_07570)	comGA	1522206..1523180 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
PDI59_RS07570 (PDI59_07575)	-	1523232..1523855 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
PDI59_RS07575 (PDI59_07580)	-	1523852..1524181 (-)	330	WP_001018871.1	MTH1187 family thiamine-binding protein	-
PDI59_RS07580 (PDI59_07585)	-	1524181..1525167 (-)	987	WP_000161311.1	ROK family glucokinase	-

Sequence

Protein

Download Length: 165 a.a. Molecular weight: 19181.99 Da Isoelectric Point: 10.1964

                                >NTDB_id=772433 PDI59_RS07540 WP_001788897.1 1519714..1520211(-) (comGF) [Staphylococcus aureus strain HP20814-062]

MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISIILLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI

LEDFKGVDKNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITMINNVTAFTANIYYKSIIKITITVKVGTNLQT

KTIYV

Nucleotide

Download Length: 498 bp

                                >NTDB_id=772433 PDI59_RS07540 WP_001788897.1 1519714..1520211(-) (comGF) [Staphylococcus aureus strain HP20814-062]

ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT

TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGTATAATTTTACTAATTGTTCCAGACT

TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT

CTAGAGGATTTTAAAGGTGTAGATAAAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT

GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACAATGATTAATAATGTTA

CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT

AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(28-51)

Putative Competence protein ComGF (ComGF)

(69-155)

Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.

3D structure

Source	ID	Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.

Visualization of predicted probability:

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	H_a-value
comGF	Staphylococcus aureus N315	100	100	1
comGF	Staphylococcus aureus MW2	96.364	100	0.964

Detailed information NTG0772433