Detailed information
Bioinformatically predicted
Overview
| Name | comEA | Type | Machinery gene |
| Locus tag | O6U12_RS04195 | Genome accession | NZ_CP114899 |
| Coordinates | 778065..778682 (+) | Length | 205 a.a. |
| NCBI ID | WP_017696282.1 | Uniprot ID | - |
| Organism | Bacillus subtilis strain | ||
| Function | dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake |
||
Related MGE
Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.
Gene-MGE association summary
| MGE type | MGE coordinates | Gene coordinates | Relative position | Distance (bp) |
|---|---|---|---|---|
| Prophage | 741234..783099 | 778065..778682 | within | 0 |
Gene organization within MGE regions
Location: 741234..783099
| Locus tag | Gene name | Coordinates (strand) | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| O6U12_RS03990 (O6U12_03990) | yqcE | 741234..741398 (+) | 165 | WP_024122146.1 | XkdX family protein | - |
| O6U12_RS03995 (O6U12_03995) | xepA | 741487..742380 (+) | 894 | WP_129093405.1 | phage-like element PBSX protein XepA | - |
| O6U12_RS04000 (O6U12_04000) | skhD | 742426..742848 (+) | 423 | WP_003246208.1 | phage holin family protein | - |
| O6U12_RS04005 (O6U12_04005) | - | 742893..743861 (+) | 969 | WP_129093404.1 | N-acetylmuramoyl-L-alanine amidase | - |
| O6U12_RS04010 (O6U12_04010) | - | 743995..744987 (+) | 993 | WP_224912955.1 | HNH endonuclease | - |
| O6U12_RS04015 (O6U12_04015) | - | 745007..745552 (+) | 546 | WP_069322772.1 | SMI1/KNR4 family protein | - |
| O6U12_RS04020 (O6U12_04020) | - | 745746..746198 (-) | 453 | WP_014480333.1 | SMI1/KNR4 family protein | - |
| O6U12_RS04025 (O6U12_04025) | - | 746297..746737 (-) | 441 | WP_129093403.1 | SMI1/KNR4 family protein | - |
| O6U12_RS04030 (O6U12_04030) | - | 746747..747139 (-) | 393 | WP_019715065.1 | immunity 50 family protein | - |
| O6U12_RS04035 (O6U12_04035) | yqcF | 747247..747825 (-) | 579 | WP_129093402.1 | type VII secretion system immunity protein YqcF | - |
| O6U12_RS04040 (O6U12_04040) | - | 747917..748348 (-) | 432 | WP_129093401.1 | SMI1/KNR4 family protein | - |
| O6U12_RS04045 (O6U12_04045) | - | 748345..748716 (-) | 372 | WP_224912977.1 | hypothetical protein | - |
| O6U12_RS04050 (O6U12_04050) | - | 748708..749997 (-) | 1290 | Protein_783 | ribonuclease YeeF family protein | - |
| O6U12_RS04055 (O6U12_04055) | - | 750184..751311 (+) | 1128 | WP_129093400.1 | Rap family tetratricopeptide repeat protein | - |
| O6U12_RS04060 (O6U12_04060) | - | 751800..752711 (+) | 912 | WP_069487169.1 | hypothetical protein | - |
| O6U12_RS04065 (O6U12_04065) | - | 752638..756258 (+) | 3621 | WP_128747171.1 | DEAD/DEAH box helicase | - |
| O6U12_RS04070 (O6U12_04070) | - | 756930..758045 (-) | 1116 | WP_017694944.1 | IS4 family transposase | - |
| O6U12_RS04075 (O6U12_04075) | - | 758227..759951 (+) | 1725 | WP_269767453.1 | AAA family ATPase | - |
| O6U12_RS04080 (O6U12_04080) | - | 760213..760391 (+) | 179 | Protein_789 | hypothetical protein | - |
| O6U12_RS04085 (O6U12_04085) | spoIVCA | 760349..761809 (+) | 1461 | WP_224912975.1 | site-specific DNA recombinase SpoIVCA | - |
| O6U12_RS04090 (O6U12_04090) | - | 761996..763408 (-) | 1413 | WP_129093397.1 | MDR family MFS transporter | - |
| O6U12_RS04095 (O6U12_04095) | - | 763558..764028 (-) | 471 | WP_015251668.1 | MarR family transcriptional regulator | - |
| O6U12_RS04100 (O6U12_04100) | - | 764168..764518 (-) | 351 | Protein_793 | sigma-70 family RNA polymerase sigma factor | - |
| O6U12_RS04105 (O6U12_04105) | nucB | 764714..765124 (+) | 411 | Protein_794 | sporulation-specific Dnase NucB | - |
| O6U12_RS04110 (O6U12_04110) | yqeB | 765158..765880 (-) | 723 | WP_026113675.1 | hypothetical protein | - |
| O6U12_RS04115 (O6U12_04115) | - | 766023..767051 (+) | 1029 | WP_017696289.1 | IS1595 family transposase | - |
| O6U12_RS04120 (O6U12_04120) | - | 767101..767523 (+) | 423 | WP_017696288.1 | hypothetical protein | - |
| O6U12_RS04125 (O6U12_04125) | gnd | 767863..768755 (+) | 893 | Protein_798 | phosphogluconate dehydrogenase (NAD(+)-dependent, decarboxylating) | - |
| O6U12_RS04130 (O6U12_04130) | yqeD | 768774..769400 (-) | 627 | WP_014480319.1 | TVP38/TMEM64 family protein | - |
| O6U12_RS04135 (O6U12_04135) | cwlH | 769587..770339 (+) | 753 | WP_017696287.1 | N-acetylmuramoyl-L-alanine amidase CwlH | - |
| O6U12_RS04140 (O6U12_04140) | yqeF | 770591..771322 (+) | 732 | WP_003229964.1 | SGNH/GDSL hydrolase family protein | - |
| O6U12_RS04145 (O6U12_04145) | - | 771581..771721 (-) | 141 | WP_003226124.1 | sporulation histidine kinase inhibitor Sda | - |
| O6U12_RS04150 (O6U12_04150) | yqeG | 772083..772601 (+) | 519 | WP_003226126.1 | YqeG family HAD IIIA-type phosphatase | - |
| O6U12_RS04155 (O6U12_04155) | yqeH | 772605..773705 (+) | 1101 | WP_003229966.1 | ribosome biogenesis GTPase YqeH | - |
| O6U12_RS04160 (O6U12_04160) | aroE | 773723..774565 (+) | 843 | WP_017696285.1 | shikimate dehydrogenase | - |
| O6U12_RS04165 (O6U12_04165) | yhbY | 774559..774849 (+) | 291 | WP_003226133.1 | ribosome assembly RNA-binding protein YhbY | - |
| O6U12_RS04170 (O6U12_04170) | nadD | 774861..775430 (+) | 570 | WP_004398676.1 | nicotinate-nucleotide adenylyltransferase | - |
| O6U12_RS04175 (O6U12_04175) | yqeK | 775420..775980 (+) | 561 | WP_017696284.1 | bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK | - |
| O6U12_RS04180 (O6U12_04180) | rsfS | 775998..776354 (+) | 357 | WP_014480315.1 | ribosome silencing factor | - |
| O6U12_RS04185 (O6U12_04185) | yqeM | 776351..777094 (+) | 744 | WP_017696283.1 | class I SAM-dependent DNA methyltransferase | - |
| O6U12_RS04190 (O6U12_04190) | comER | 777160..777981 (-) | 822 | WP_004398597.1 | late competence protein ComER | - |
| O6U12_RS04195 (O6U12_04195) | comEA | 778065..778682 (+) | 618 | WP_017696282.1 | competence protein ComEA | Machinery gene |
| O6U12_RS04200 (O6U12_04200) | comEB | 778749..779318 (+) | 570 | WP_003229978.1 | ComE operon protein 2 | - |
| O6U12_RS04205 (O6U12_04205) | comEC | 779322..781652 (+) | 2331 | WP_017696281.1 | DNA internalization-related competence protein ComEC/Rec2 | Machinery gene |
| O6U12_RS04210 (O6U12_04210) | yqzM | 781692..781826 (-) | 135 | WP_003229983.1 | YqzM family protein | - |
| O6U12_RS04215 (O6U12_04215) | - | 781867..782016 (+) | 150 | WP_003229985.1 | hypothetical protein | - |
| O6U12_RS04220 (O6U12_04220) | holA | 782056..783099 (+) | 1044 | WP_017696280.1 | DNA polymerase III subunit delta | - |
Sequence
Protein
Download Length: 205 a.a. Molecular weight: 21763.49 Da Isoelectric Point: 4.7220
>NTDB_id=767567 O6U12_RS04195 WP_017696282.1 778065..778682(+) (comEA) [Bacillus subtilis strain]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEGNNDESNEIIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTEAENTVVKQEGNNDESNEIIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGPLVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK
Nucleotide
Download Length: 618 bp
>NTDB_id=767567 O6U12_RS04195 WP_017696282.1 778065..778682(+) (comEA) [Bacillus subtilis strain]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGAAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGGAA
ACAACGACGAGTCAAACGAAATAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGACCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGAAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGGCAGAAAATACAGTGGTAAAGCAGGAAGGAA
ACAACGACGAGTCAAACGAAATAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGACCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATATCGGGAAGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA
3D structure
| Source | ID | Structure |
|---|
Similar proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|---|---|---|---|
| comEA | Bacillus subtilis subsp. subtilis str. 168 |
98.049 |
100 |
0.98 |
| comEA | Staphylococcus aureus MW2 |
37.273 |
100 |
0.4 |
| comEA | Staphylococcus aureus N315 |
36.818 |
100 |
0.395 |