Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   O4M85_RS26510 Genome accession   NZ_CP114761
Coordinates   5641707..5643299 (+) Length   530 a.a.
NCBI ID   WP_039028118.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain NF143349     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5636707..5648299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4M85_RS26490 (O4M85_26490) pgeF 5638457..5639185 (-) 729 WP_033972914.1 peptidoglycan editing factor PgeF -
  O4M85_RS26495 (O4M85_26495) rluD 5639182..5640144 (-) 963 WP_033972915.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  O4M85_RS26500 (O4M85_26500) - 5640290..5641315 (+) 1026 WP_033972916.1 outer membrane protein assembly factor BamD -
  O4M85_RS26505 (O4M85_26505) - 5641472..5641717 (+) 246 WP_003102591.1 PP0621 family protein -
  O4M85_RS26510 (O4M85_26510) pilS 5641707..5643299 (+) 1593 WP_039028118.1 two-component system sensor histidine kinase PilS Regulator
  O4M85_RS26515 (O4M85_26515) pilR 5643314..5644651 (+) 1338 WP_003094694.1 two-component system response regulator PilR Regulator
  O4M85_RS26520 (O4M85_26520) thiO 5644697..5645791 (-) 1095 WP_088376838.1 glycine oxidase ThiO -
  O4M85_RS26525 (O4M85_26525) - 5645926..5646435 (+) 510 WP_033976381.1 GspH/FimT family pseudopilin -
  O4M85_RS26530 (O4M85_26530) - 5646541..5647047 (+) 507 WP_003134935.1 GspH/FimT family pseudopilin -
  O4M85_RS26535 (O4M85_26535) pilV 5647038..5647595 (+) 558 WP_003125211.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59116.28 Da        Isoelectric Point: 6.4439

>NTDB_id=766959 O4M85_RS26510 WP_039028118.1 5641707..5643299(+) (pilS) [Pseudomonas aeruginosa strain NF143349]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEDQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLSSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLG
RHSPMLMHCMKQWRLNPSLRPPTLKVLPDGPTVQPSFISLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLQRFVDEYPGRLRND
SELHLQLGADDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLVRDPESDLPVLEVIDDGPGVPTDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=766959 O4M85_RS26510 WP_039028118.1 5641707..5643299(+) (pilS) [Pseudomonas aeruginosa strain NF143349]
GTGCGCGCTGAACGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGTCTGTACCACCTGTACCGCCTGACCAT
AGGCCTGGTACTGGTCCTGCTGATCTCCAGCGAACTGGAAGATCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGTTGGTGCTACCTGGTCTTCAACATCCTGGTCGCGCTGTTCCTGCCGCCGTCGCGGCAATTGCTGCCGATCTTC
ATCCTCGCGCTCACCGACGTGCTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAATATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCAGCGGCCAGCCTCG
GGCTGCTCTACCTGACCTTCTTCCTCAGCCTGAGCAGCCCGGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGC
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTACGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAAGAACTCAACGCATTGATCCTGCAGCGTATGCGCACCGGCATCCTCGTGGTCGATA
GCCGTCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTACAGGGCGCCAGCCTCGGC
CGCCACAGCCCGATGCTGATGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCACCGACGCTCAAGGTGCT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCAGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGTCTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAGCTGCTGCAGGAGTCCGAGGAGCTGGACGCCCCGGACCGGCG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTTCAGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAT
AGCGAACTGCACCTGCAACTCGGTGCCGACGACATCCAGACCCGCATGGACCCACACCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGTGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTACCGACGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGCACCGGCCTGGGCCTCTATCTCTCCCGCGAGCTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

99.057

100

0.991