Detailed information    

insolico Bioinformatically predicted

Overview


Name   ssb   Type   Machinery gene
Locus tag   O4N81_RS00040 Genome accession   NZ_CP114617
Coordinates   9264..9776 (+) Length   170 a.a.
NCBI ID   WP_002288368.1    Uniprot ID   -
Organism   Enterococcus faecium strain EF0656     
Function   ssDNA binding (predicted from homology)   
DNA processing

Related MGE


Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
ICE 3145..79324 9264..9776 within 0


Gene organization within MGE regions


Location: 3145..79324
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4N81_RS00025 (O4N81_00025) gyrB 4242..6188 (+) 1947 WP_002288364.1 DNA topoisomerase (ATP-hydrolyzing) subunit B -
  O4N81_RS00030 (O4N81_00030) gyrA 6208..8679 (+) 2472 WP_002321575.1 DNA gyrase subunit A -
  O4N81_RS00035 (O4N81_00035) rpsF 8915..9214 (+) 300 WP_002288366.1 30S ribosomal protein S6 -
  O4N81_RS00040 (O4N81_00040) ssb 9264..9776 (+) 513 WP_002288368.1 single-stranded DNA-binding protein Machinery gene
  O4N81_RS00045 (O4N81_00045) rpsR 9802..10038 (+) 237 WP_002288370.1 30S ribosomal protein S18 -
  O4N81_RS00050 (O4N81_00050) - 10181..12154 (+) 1974 WP_002288371.1 DHH family phosphoesterase -
  O4N81_RS00055 (O4N81_00055) rplI 12160..12612 (+) 453 WP_002288372.1 50S ribosomal protein L9 -
  O4N81_RS00060 (O4N81_00060) dnaB 12886..14253 (+) 1368 WP_002288373.1 replicative DNA helicase -
  O4N81_RS00065 (O4N81_00065) - 15033..16325 (+) 1293 WP_002288375.1 adenylosuccinate synthase -
  O4N81_RS00070 (O4N81_00070) - 17112..17975 (+) 864 WP_002288377.1 DegV family protein -
  O4N81_RS00075 (O4N81_00075) - 18104..19228 (-) 1125 Protein_14 ABC-F family ATP-binding cassette domain-containing protein -
  O4N81_RS00080 (O4N81_00080) - 19283..19963 (-) 681 WP_001015311.1 IS6-like element IS1216 family transposase -
  O4N81_RS00085 (O4N81_00085) - 20152..21516 (+) 1365 Protein_16 ABC transporter ATP-binding protein -
  O4N81_RS00090 (O4N81_00090) rpsN 22039..22308 (+) 270 WP_002297316.1 30S ribosomal protein S14 -
  O4N81_RS00095 (O4N81_00095) rpmF 22323..22502 (+) 180 WP_002297318.1 50S ribosomal protein L32 -
  O4N81_RS00100 (O4N81_00100) - 22534..22911 (+) 378 WP_002297319.1 hypothetical protein -
  O4N81_RS00105 (O4N81_00105) rpmG 22932..23081 (+) 150 WP_002297320.1 50S ribosomal protein L33 -
  O4N81_RS00110 (O4N81_00110) - 23105..24088 (+) 984 WP_002304819.1 CobW family GTP-binding protein -
  O4N81_RS00115 (O4N81_00115) - 24118..24240 (+) 123 WP_002322279.1 putative metal homeostasis protein -
  O4N81_RS00120 (O4N81_00120) - 24258..25781 (+) 1524 WP_269528370.1 zinc ABC transporter substrate-binding protein AdcA -
  O4N81_RS00125 (O4N81_00125) - 25805..26044 (+) 240 WP_002304820.1 helix-turn-helix domain-containing protein -
  O4N81_RS00130 (O4N81_00130) - 26237..26698 (-) 462 WP_002297325.1 IS30 family transposase -
  O4N81_RS00135 (O4N81_00135) - 26893..27111 (-) 219 WP_002317220.1 helix-turn-helix domain-containing protein -
  O4N81_RS00140 (O4N81_00140) - 27229..28845 (+) 1617 WP_269528371.1 GTP-binding protein -
  O4N81_RS00145 (O4N81_00145) - 29086..30426 (-) 1341 Protein_28 ISL3 family transposase -
  O4N81_RS15175 - 30864..31010 (-) 147 WP_138816292.1 transposase family protein -
  O4N81_RS15275 - 31049..31243 (-) 195 WP_002297332.1 IS3 family transposase -
  O4N81_RS00150 (O4N81_00150) - 31153..31485 (-) 333 WP_002311665.1 helix-turn-helix domain-containing protein -
  O4N81_RS00155 (O4N81_00155) - 31999..32190 (-) 192 WP_002304825.1 helix-turn-helix domain-containing protein -
  O4N81_RS00160 (O4N81_00160) - 32284..32670 (-) 387 WP_002297333.1 sigma-70 family RNA polymerase sigma factor -
  O4N81_RS00165 (O4N81_00165) - 33364..34569 (+) 1206 WP_002297334.1 YSIRK-targeted surface antigen transcriptional regulator -
  O4N81_RS00170 (O4N81_00170) - 34586..35020 (+) 435 WP_002332818.1 DUF7006 family protein -
  O4N81_RS00175 (O4N81_00175) esp 35637..42056 (+) 6420 WP_269528372.1 repetitive surface protein Esp -
  O4N81_RS00180 (O4N81_00180) - 42223..43518 (-) 1296 WP_002297218.1 ISL3-like element ISEfa11 family transposase -
  O4N81_RS00185 (O4N81_00185) - 43712..45043 (+) 1332 WP_010729817.1 FAD/NAD(P)-binding oxidoreductase -
  O4N81_RS00190 (O4N81_00190) - 45030..45989 (-) 960 WP_002303350.1 IS30 family transposase -
  O4N81_RS00195 (O4N81_00195) - 46233..47684 (+) 1452 WP_002297338.1 glucosaminidase domain-containing protein -
  O4N81_RS00200 (O4N81_00200) - 48033..48665 (+) 633 WP_010729820.1 hypothetical protein -
  O4N81_RS00205 (O4N81_00205) - 49253..50692 (+) 1440 WP_002297342.1 MFS transporter -
  O4N81_RS00210 (O4N81_00210) - 50987..51205 (+) 219 Protein_43 zinc ribbon domain-containing protein -
  O4N81_RS00215 (O4N81_00215) - 51454..51669 (+) 216 WP_002297343.1 hypothetical protein -
  O4N81_RS00220 (O4N81_00220) - 51906..52142 (-) 237 Protein_45 transposase -
  O4N81_RS00225 (O4N81_00225) - 52329..53240 (-) 912 WP_002297345.1 conjugal transfer protein -
  O4N81_RS00230 (O4N81_00230) - 53259..54263 (-) 1005 WP_002297346.1 lysozyme family protein -
  O4N81_RS00235 (O4N81_00235) - 54260..56383 (-) 2124 WP_002297347.1 FUSC family protein -
  O4N81_RS00240 (O4N81_00240) - 56388..58835 (-) 2448 WP_010729283.1 ATP-binding protein -
  O4N81_RS00245 (O4N81_00245) - 58822..59211 (-) 390 WP_002297349.1 conjugal transfer protein -
  O4N81_RS00250 (O4N81_00250) - 59271..59774 (-) 504 WP_002297350.1 antirestriction protein ArdA -
  O4N81_RS00255 (O4N81_00255) - 59787..60011 (-) 225 WP_033658092.1 hypothetical protein -
  O4N81_RS00260 (O4N81_00260) ltrA 60115..62025 (-) 1911 WP_002286940.1 group II intron reverse transcriptase/maturase -
  O4N81_RS00265 (O4N81_00265) - 62771..62908 (-) 138 WP_002317225.1 DUF3789 domain-containing protein -
  O4N81_RS00270 (O4N81_00270) mobT 62905..64089 (-) 1185 WP_002297353.1 MobT family relaxase -
  O4N81_RS00275 (O4N81_00275) - 64348..64638 (-) 291 WP_002297354.1 hypothetical protein -
  O4N81_RS00280 (O4N81_00280) dcm 64628..65770 (-) 1143 Protein_57 DNA (cytosine-5-)-methyltransferase -
  O4N81_RS00285 (O4N81_00285) - 65846..66010 (-) 165 WP_002297357.1 hypothetical protein -
  O4N81_RS00290 (O4N81_00290) - 66003..66677 (-) 675 WP_002297358.1 zeta toxin family protein -
  O4N81_RS00295 (O4N81_00295) - 66755..68095 (-) 1341 WP_010729284.1 FtsK/SpoIIIE domain-containing protein -
  O4N81_RS00300 (O4N81_00300) - 68178..68513 (-) 336 WP_002297364.1 hypothetical protein -
  O4N81_RS00305 (O4N81_00305) - 68523..68897 (-) 375 WP_002297365.1 YdcP family protein -
  O4N81_RS00310 (O4N81_00310) - 68910..69224 (-) 315 WP_002297366.1 YdcP family protein -
  O4N81_RS00315 (O4N81_00315) fss3 69337..72564 (-) 3228 WP_010729821.1 fibrinogen-binding MSCRAMM adhesin Fss3 -
  O4N81_RS00320 (O4N81_00320) - 73421..73675 (+) 255 WP_002303696.1 hypothetical protein -
  O4N81_RS00325 (O4N81_00325) - 73839..74756 (+) 918 WP_002287502.1 ATP-binding cassette domain-containing protein -
  O4N81_RS00330 (O4N81_00330) - 74749..75531 (+) 783 WP_002303697.1 ABC transporter permease subunit -
  O4N81_RS00335 (O4N81_00335) - 75532..76212 (+) 681 WP_002303698.1 response regulator transcription factor -
  O4N81_RS00340 (O4N81_00340) - 76205..77101 (+) 897 WP_002303699.1 sensor histidine kinase -
  O4N81_RS00345 (O4N81_00345) - 77313..79235 (+) 1923 WP_002303700.1 BglG family transcription antiterminator -

Sequence


Protein


Download         Length: 170 a.a.        Molecular weight: 18451.99 Da        Isoelectric Point: 4.6742

>NTDB_id=766862 O4N81_RS00040 WP_002288368.1 9264..9776(+) (ssb) [Enterococcus faecium strain EF0656]
MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNREADFINCVIWRKSAETLANYARKGTLLGVTGRIQTR
SYDNQQGQRVYVTEVVADNFQLLESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
IDISDDDLPF

Nucleotide


Download         Length: 513 bp        

>NTDB_id=766862 O4N81_RS00040 WP_002288368.1 9264..9776(+) (ssb) [Enterococcus faecium strain EF0656]
TTGATTAATAATGTTGTATTAGTTGGACGTCTGACGAAAGATCCAGATTTGCGTTACACAGCAAGTGGTACGGCAGTCGC
AACATTTACTTTGGCTGTGAATCGTAATTTTACAAACCAAAGCGGCAATCGCGAGGCTGATTTTATCAACTGTGTGATTT
GGCGCAAATCAGCAGAAACTTTAGCAAACTATGCTCGTAAAGGAACACTTTTAGGTGTTACTGGACGTATCCAAACTCGT
TCTTATGATAATCAGCAAGGACAACGTGTATACGTGACAGAAGTAGTAGCTGACAACTTCCAATTGCTGGAAAGTCGTTC
TGCATCTGAAAATCGCCAACAAAGCGGCGGATTCCAAAGTTCTGGTCAATCAGCTGGCGGATTTGGCGGAAACAATAACT
CTAATCAAACATCTCAATCATCCAACGGTATGCCTGATTTCGACCGTGATACTTCTGACCCATTCGGCTCAAGTTCAACG
ATCGACATTTCAGACGATGATTTACCATTCTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  ssb Latilactobacillus sakei subsp. sakei 23K

59.322

100

0.618

  ssbA Bacillus subtilis subsp. subtilis str. 168

56.983

100

0.6

  ssbB Bacillus subtilis subsp. subtilis str. 168

62.264

62.353

0.388