Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   QMK40_RS29780 Genome accession   NZ_CP125361
Coordinates   6395166..6395789 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain ZY94     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6390166..6400789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QMK40_RS29760 (QMK40_29760) aroB 6390761..6391867 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  QMK40_RS29765 (QMK40_29765) aroK 6391917..6392435 (-) 519 WP_003095833.1 shikimate kinase AroK -
  QMK40_RS29770 (QMK40_29770) pilQ 6392447..6394591 (-) 2145 WP_003123619.1 type 4a pilus secretin PilQ Machinery gene
  QMK40_RS29775 (QMK40_29775) pilP 6394645..6395169 (-) 525 WP_223111084.1 type 4a pilus biogenesis lipoprotein PilP -
  QMK40_RS29780 (QMK40_29780) pilO 6395166..6395789 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  QMK40_RS29785 (QMK40_29785) pilN 6395786..6396382 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  QMK40_RS29790 (QMK40_29790) pilM 6396382..6397446 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  QMK40_RS29795 (QMK40_29795) - 6397631..6400102 (+) 2472 WP_031756300.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=762637 QMK40_RS29780 WP_003103268.1 6395166..6395789(-) (pilO) [Pseudomonas aeruginosa strain ZY94]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=762637 QMK40_RS29780 WP_003103268.1 6395166..6395789(-) (pilO) [Pseudomonas aeruginosa strain ZY94]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTTGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401