Detailed information    

insolico Bioinformatically predicted

Overview

Name   comEA   Type   Machinery gene
Locus tag   OVA33_RS12215 Genome accession   NZ_CP113515
Coordinates   2364763..2365377 (+) Length   204 a.a.
NCBI ID   WP_013352942.1    Uniprot ID   A0A411A874
Organism   Bacillus sp. KICET-1     
Function   dsDNA binding to the cell surface (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2359763..2370377
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OVA33_RS12180 (OVA33_12185) aroE 2360450..2361292 (+) 843 WP_017418183.1 shikimate dehydrogenase -
  OVA33_RS12185 (OVA33_12190) yhbY 2361286..2361576 (+) 291 WP_003152858.1 ribosome assembly RNA-binding protein YhbY -
  OVA33_RS12190 (OVA33_12195) - 2361587..2362156 (+) 570 WP_017418182.1 nicotinate-nucleotide adenylyltransferase -
  OVA33_RS12195 (OVA33_12200) yqeK 2362146..2362727 (+) 582 WP_017418181.1 bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK -
  OVA33_RS12200 (OVA33_12205) rsfS 2362724..2363080 (+) 357 WP_003152864.1 ribosome silencing factor -
  OVA33_RS12205 (OVA33_12210) - 2363077..2363814 (+) 738 WP_014305450.1 class I SAM-dependent methyltransferase -
  OVA33_RS12210 (OVA33_12215) comER 2363883..2364704 (-) 822 WP_014305449.1 late competence protein ComER -
  OVA33_RS12215 (OVA33_12220) comEA 2364763..2365377 (+) 615 WP_013352942.1 helix-hairpin-helix domain-containing protein Machinery gene
  OVA33_RS12220 (OVA33_12225) - 2365444..2366013 (+) 570 WP_003152868.1 ComE operon protein 2 -
  OVA33_RS12225 (OVA33_12230) comEC 2366014..2368365 (+) 2352 WP_025649830.1 DNA internalization-related competence protein ComEC/Rec2 Machinery gene
  OVA33_RS12230 (OVA33_12235) - 2368384..2368518 (-) 135 WP_003152870.1 YqzM family protein -
  OVA33_RS12235 (OVA33_12240) - 2368559..2368710 (+) 152 Protein_2350 hypothetical protein -
  OVA33_RS12240 (OVA33_12245) holA 2368750..2369845 (+) 1096 Protein_2351 DNA polymerase III subunit delta -
  OVA33_RS12245 (OVA33_12250) rpsT 2369808..2370074 (-) 267 WP_003152876.1 30S ribosomal protein S20 -

Sequence

Protein


Download         Length: 204 a.a.        Molecular weight: 22190.27 Da        Isoelectric Point: 5.4841

>NTDB_id=762531 OVA33_RS12215 WP_013352942.1 2364763..2365377(+) (comEA) [Bacillus sp. KICET-1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTADDKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTIVYIPSEGEEENHPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide


Download         Length: 615 bp        

>NTDB_id=762531 OVA33_RS12215 WP_013352942.1 2364763..2365377(+) (comEA) [Bacillus sp. KICET-1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGTATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCGGCAT
CCGTCGCGGACAAAAAAGACACCGCAGACGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGAGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATTGTTTATATTCCGAGTGAAGGCGAAGAAGAGAATCACCCGAAAACAG
CAGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTTTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCCATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

Helix-hairpin-helix motif (HHH_3)

(141-202)

SLBB domain (SLBB)

(68-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A411A874

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comEA Bacillus subtilis subsp. subtilis str. 168

58.854

94.118

0.554
  comEA/celA/cilE Streptococcus pneumoniae TIGR4

35.545

100

0.368
  comEA/celA/cilE Streptococcus mitis SK321

35.071

100

0.363