Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   OS912_RS06220 Genome accession   NZ_CP113266
Coordinates   1224024..1225322 (-) Length   432 a.a.
NCBI ID   WP_044813853.1    Uniprot ID   -
Organism   Streptococcus pneumoniae strain BC1     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1219024..1230322
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OS912_RS06200 (OS912_06205) rplI 1220179..1220631 (-) 453 WP_000864220.1 50S ribosomal protein L9 -
  OS912_RS06205 (OS912_06210) - 1220628..1222601 (-) 1974 WP_000715124.1 DHH family phosphoesterase -
  OS912_RS06210 (OS912_06215) hpf 1222737..1223285 (-) 549 WP_000599107.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  OS912_RS06215 (OS912_06220) comFC/cflB 1223365..1224027 (-) 663 WP_000649971.1 ComF family protein Machinery gene
  OS912_RS06220 (OS912_06225) comFA/cflA 1224024..1225322 (-) 1299 WP_044813853.1 DEAD/DEAH box helicase Machinery gene
  OS912_RS06225 (OS912_06230) - 1225378..1226013 (+) 636 WP_268232847.1 YigZ family protein -
  OS912_RS06230 (OS912_06235) cysK 1226298..1227218 (+) 921 WP_000029860.1 cysteine synthase A -
  OS912_RS06235 (OS912_06240) - 1227293..1227831 (-) 539 Protein_1199 IS66 family transposase -
  OS912_RS06240 (OS912_06245) - 1227804..1228091 (+) 288 Protein_1200 transposase -
  OS912_RS06245 (OS912_06250) tnpB 1228268..1228555 (-) 288 WP_000586646.1 IS66 family insertion sequence element accessory protein TnpB -
  OS912_RS06250 (OS912_06255) tsf 1228801..1229841 (-) 1041 WP_050250816.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49504.52 Da        Isoelectric Point: 8.7540

>NTDB_id=761426 OS912_RS06220 WP_044813853.1 1224024..1225322(-) (comFA/cflA) [Streptococcus pneumoniae strain BC1]
MKVNLDYLGRLFTENELTAEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLDLYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPMLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLNLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLAEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVIEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=761426 OS912_RS06220 WP_044813853.1 1224024..1225322(-) (comFA/cflA) [Streptococcus pneumoniae strain BC1]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGCAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGTTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCAAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGATTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGATCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGGATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCATGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTTCGTTTAGGAGAACTAAAAAGACTGAATTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGCAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAATAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae D39

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae R6

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae TIGR4

99.074

100

0.991
  comFA/cflA Streptococcus mitis NCTC 12261

92.824

100

0.928
  comFA/cflA Streptococcus mitis SK321

91.898

100

0.919
  comFA Lactococcus lactis subsp. cremoris KW2

52.451

94.444

0.495
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387