Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   QKA38_RS27775 Genome accession   NZ_CP124669
Coordinates   5978368..5978991 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 2021CK-01494     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5973368..5983991
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QKA38_RS27755 (QKA38_27755) aroB 5973963..5975069 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  QKA38_RS27760 (QKA38_27760) aroK 5975119..5975637 (-) 519 WP_003095833.1 shikimate kinase AroK -
  QKA38_RS27765 (QKA38_27765) pilQ 5975649..5977793 (-) 2145 WP_023098191.1 type 4a pilus secretin PilQ Machinery gene
  QKA38_RS27770 (QKA38_27770) comL/pilP 5977847..5978371 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  QKA38_RS27775 (QKA38_27775) pilO 5978368..5978991 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  QKA38_RS27780 (QKA38_27780) pilN 5978988..5979584 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  QKA38_RS27785 (QKA38_27785) pilM 5979584..5980648 (-) 1065 WP_003110888.1 type IV pilus assembly protein PilM Machinery gene
  QKA38_RS27790 (QKA38_27790) - 5980833..5983304 (+) 2472 WP_023098192.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=761101 QKA38_RS27775 WP_003103268.1 5978368..5978991(-) (pilO) [Pseudomonas aeruginosa strain 2021CK-01494]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=761101 QKA38_RS27775 WP_003103268.1 5978368..5978991(-) (pilO) [Pseudomonas aeruginosa strain 2021CK-01494]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGCGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTGGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401