Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   OTU47_RS02475 Genome accession   NZ_CP113115
Coordinates   480193..481491 (+) Length   432 a.a.
NCBI ID   WP_000867612.1    Uniprot ID   A0A0E9GXK4
Organism   Streptococcus pneumoniae strain NP2     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 475193..486491
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTU47_RS02445 (OTU47_02445) tsf 475660..476700 (+) 1041 WP_001862774.1 translation elongation factor Ts -
  OTU47_RS02450 (OTU47_02450) tnpB 477054..477269 (+) 216 WP_235786183.1 IS66 family insertion sequence element accessory protein TnpB -
  OTU47_RS02455 (OTU47_02455) - 477424..477711 (-) 288 Protein_477 transposase -
  OTU47_RS02460 (OTU47_02460) - 477684..478222 (+) 539 Protein_478 transposase -
  OTU47_RS02465 (OTU47_02465) cysK 478297..479217 (-) 921 WP_000029869.1 cysteine synthase A -
  OTU47_RS02470 (OTU47_02470) - 479502..480137 (-) 636 WP_000395739.1 YigZ family protein -
  OTU47_RS02475 (OTU47_02475) comFA/cflA 480193..481491 (+) 1299 WP_000867612.1 DEAD/DEAH box helicase Machinery gene
  OTU47_RS02480 (OTU47_02480) comFC/cflB 481488..482150 (+) 663 WP_000649978.1 ComF family protein Machinery gene
  OTU47_RS02485 (OTU47_02485) hpf 482230..482778 (+) 549 WP_000599104.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  OTU47_RS02490 (OTU47_02490) - 482914..484887 (+) 1974 WP_000715109.1 DHH family phosphoesterase -
  OTU47_RS02495 (OTU47_02495) rplI 484884..485336 (+) 453 WP_000864220.1 50S ribosomal protein L9 -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49563.48 Da        Isoelectric Point: 8.4382

>NTDB_id=760399 OTU47_RS02475 WP_000867612.1 480193..481491(+) (comFA/cflA) [Streptococcus pneumoniae strain NP2]
MKVNLDYLGRLFTENELTEEERQLAEKLPAMRKEKGKLFCQRCNSTILEEWYLPIGAYYCRECLLMKRVRSDQTLYYFPQ
EDFPKQDVLKWRGQLTPFQEKVSEGLLQAVDKQKPTLVHAVTGAGKTEMIYQVVAKVINAGGAVCLASPRIDVCLELYKR
LQQDFSCGIALLHGESEPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYHAVKNSVKENGLRIFLTATST
NELDKKVRLGELKRLSLPRRFHGNPLIIPKPIWLSDFNRYLDKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLEEI
LQEQFPNEKIGFVSSVTEDRLEQVQAFRDGELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGLNASIKKAIKEIQMMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=760399 OTU47_RS02475 WP_000867612.1 480193..481491(+) (comFA/cflA) [Streptococcus pneumoniae strain NP2]
ATGAAAGTAAATTTAGATTATCTCGGTCGTTTATTTACTGAGAATGAATTAACAGAAGAAGAACGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAGGGGAAACTTTTCTGTCAACGCTGTAATAGTACTATTCTAGAAGAATGGTATTTGC
CCATCGGTGCTTACTATTGTCGAGAGTGCTTGCTGATGAAGCGAGTCAGAAGTGATCAAACTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTCAAATGGCGCGGCCAATTAACTCCTTTTCAAGAGAAGGTGTCAGAGGGATTGCT
TCAAGCAGTAGACAAGCAAAAGCCAACCTTAGTTCATGCGGTAACAGGAGCTGGAAAGACAGAAATGATTTATCAAGTAG
TGGCTAAAGTGATCAATGCGGGTGGTGCAGTGTGTTTGGCTAGTCCTCGCATAGATGTTTGTTTGGAGCTGTACAAGCGC
CTGCAACAGGATTTTTCTTGCGGTATAGCTTTGCTACATGGAGAATCGGAACCTTATTTTCGAACACCACTAGTTGTTGC
AACAACCCATCAGTTATTGAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCTTTTCCTTATGTTG
ATAATCCCACGCTTTACCACGCTGTCAAGAATAGTGTAAAGGAGAATGGATTGAGAATCTTTTTAACAGCGACTTCGACC
AATGAGTTAGATAAAAAGGTCCGTTTAGGAGAACTAAAAAGACTGAGTTTACCGAGACGGTTTCATGGAAATCCGTTGAT
TATTCCAAAACCAATTTGGTTATCGGATTTTAATCGCTACTTAGACAAGAATCGTTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAAATTAAGAAAGGGGAGCAGTTAGAAGAAATC
TTACAGGAGCAATTTCCAAATGAGAAAATTGGCTTTGTATCTTCTGTAACAGAGGATCGATTAGAGCAAGTACAAGCTTT
TCGAGATGGAGAACTGACAATACTTATCAGTACGACAATCTTGGAGCGTGGAGTTACCTTCCCTTGTGTGGATGTTTTCG
TAGTAGAGGCCAATCATCGTTTGTTTACCAAGTCTAGTTTGATTCAGATTGGTGGACGAGTTGGACGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTCCATGATGGGTTAAATGCTTCAATCAAGAAGGCGATTAAGGAAATTCAGATGATGAA
TAAGGAGGCTGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-238)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0E9GXK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus pneumoniae Rx1

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae D39

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae R6

99.306

100

0.993
  comFA/cflA Streptococcus pneumoniae TIGR4

99.074

100

0.991
  comFA/cflA Streptococcus mitis NCTC 12261

93.519

100

0.935
  comFA/cflA Streptococcus mitis SK321

92.361

100

0.924
  comFA Lactococcus lactis subsp. cremoris KW2

51.961

94.444

0.491
  comFA Latilactobacillus sakei subsp. sakei 23K

38.747

99.769

0.387