Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   OSH15_RS02350 Genome accession   NZ_CP113074
Coordinates   476424..477164 (+) Length   246 a.a.
NCBI ID   WP_000076102.1    Uniprot ID   A0A1E3MBI9
Organism   Acinetobacter baumannii strain ATCC17978 TnAraC-H0N27_12600     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 471424..482164
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OSH15_RS02335 (OSH15_02335) ponA 472010..474565 (-) 2556 WP_000736677.1 penicillin-binding protein PBP1a -
  OSH15_RS02340 (OSH15_02340) pilM 474728..475786 (+) 1059 WP_002014066.1 pilus assembly protein PilM Machinery gene
  OSH15_RS02345 (OSH15_02345) pilN 475786..476427 (+) 642 WP_000201223.1 PilN domain-containing protein Machinery gene
  OSH15_RS02350 (OSH15_02350) pilO 476424..477164 (+) 741 WP_000076102.1 type 4a pilus biogenesis protein PilO Machinery gene
  OSH15_RS02355 (OSH15_02355) pilP 477175..477702 (+) 528 WP_000695065.1 pilus assembly protein PilP Machinery gene
  OSH15_RS02360 (OSH15_02360) pilQ 477770..479935 (+) 2166 WP_001017031.1 type IV pilus secretin PilQ Machinery gene
  OSH15_RS02365 (OSH15_02365) aroK 479947..480516 (+) 570 WP_000643712.1 shikimate kinase AroK -
  OSH15_RS02370 (OSH15_02370) aroB 480545..481627 (+) 1083 WP_001193998.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 246 a.a.        Molecular weight: 27849.61 Da        Isoelectric Point: 4.8357

>NTDB_id=759903 OSH15_RS02350 WP_000076102.1 476424..477164(+) (pilO) [Acinetobacter baumannii strain ATCC17978 TnAraC-H0N27_12600]
MSQDELQELSLEQLSAKKKKFNLDKFLQQFNTLDMNNYGSWPISVKITCWIFIFFAVLALGYFIVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQVQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
IAIEATGDYHAFGAFVSSIAALPRIVTMHDFTVDVSPAKDNKSDIPVLNYSIKAKTYRYVGTTDTSEQTGKAPAASASTN
TTVQPK

Nucleotide


Download         Length: 741 bp        

>NTDB_id=759903 OSH15_RS02350 WP_000076102.1 476424..477164(+) (pilO) [Acinetobacter baumannii strain ATCC17978 TnAraC-H0N27_12600]
ATGAGTCAAGACGAACTTCAAGAATTGTCATTAGAACAATTATCTGCAAAGAAAAAGAAATTTAATCTCGATAAATTCCT
TCAGCAATTTAATACGCTGGATATGAACAACTATGGTAGTTGGCCAATATCTGTAAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTGTTAGCTTTGGGGTATTTCATTGTTATTCAACCGAAATTACAAGCTATTGATAATGCTCAAGCTCAGGAA
AGCAATTTATTAAACGAATTTCGTGAAAAAGACTCGAAATTACGTAACCTGCAACAGTATCAGGTGCAACTTCAAGAGAT
GCAGGCAAATTTCAACCAGCAACTAGAGCAGTTACCAAAAGAAACTGAGATTCCGAGTTTAGTTGAAGATATCAACTTAA
CAGGAGTGAACTCTGGATTGAAATTTAAAAATATTCGCTTGGAAGATGAAGTAAAGCAGGAAATTTTTATTGAGCAACCA
ATTGCCATAGAAGCGACCGGGGACTATCACGCATTTGGAGCTTTTGTAAGTAGTATCGCTGCTTTGCCTCGTATTGTAAC
TATGCACGATTTTACTGTTGATGTATCGCCAGCTAAAGATAACAAGTCTGATATTCCTGTGCTGAACTATTCCATTAAAG
CAAAAACATACCGATACGTAGGGACAACAGATACATCGGAGCAAACAGGCAAAGCTCCAGCAGCATCTGCCTCAACGAAC
ACTACTGTTCAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1E3MBI9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

99.593

100

0.996
  comO Acinetobacter baylyi ADP1

69.421

98.374

0.683