Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   H7R23_RS01630 Genome accession   NZ_AP021936
Coordinates   345616..346395 (+) Length   259 a.a.
NCBI ID   WP_017481731.1    Uniprot ID   A0A9X3QKA0
Organism   Acinetobacter pittii strain WP2-W18-ESBL-11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 340616..351395
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7R23_RS01615 (WP2W18E11_03050) ponA 341197..343758 (-) 2562 WP_182917758.1 penicillin-binding protein PBP1a -
  H7R23_RS01620 (WP2W18E11_03060) pilM 343920..344978 (+) 1059 WP_005067078.1 pilus assembly protein PilM Machinery gene
  H7R23_RS01625 (WP2W18E11_03070) pilN 344978..345619 (+) 642 WP_017481732.1 PilN domain-containing protein Machinery gene
  H7R23_RS01630 (WP2W18E11_03080) pilO 345616..346395 (+) 780 WP_017481731.1 type 4a pilus biogenesis protein PilO Machinery gene
  H7R23_RS01635 (WP2W18E11_03090) pilP 346407..346934 (+) 528 WP_017481730.1 pilus assembly protein PilP Machinery gene
  H7R23_RS01640 (WP2W18E11_03100) pilQ 346997..349123 (+) 2127 WP_182917759.1 type IV pilus secretin PilQ Machinery gene
  H7R23_RS01645 (WP2W18E11_03110) aroK 349135..349704 (+) 570 WP_002116987.1 shikimate kinase AroK -
  H7R23_RS01650 (WP2W18E11_03120) aroB 349733..350815 (+) 1083 WP_017481728.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 259 a.a.        Molecular weight: 29463.60 Da        Isoelectric Point: 5.7424

>NTDB_id=75959 H7R23_RS01630 WP_017481731.1 345616..346395(+) (pilO) [Acinetobacter pittii strain WP2-W18-ESBL-11]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLVLGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFVVDAAPIKDGKSDIPVLNYSIKAKTYRYMGAVENQNNSDPQVINGESQI
NASDSRNVHIPSIKKSKRK

Nucleotide


Download         Length: 780 bp        

>NTDB_id=75959 H7R23_RS01630 WP_017481731.1 345616..346395(+) (pilO) [Acinetobacter pittii strain WP2-W18-ESBL-11]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTTCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTACTGGTTTTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAGCTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
AATGCATGATTTTGTTGTTGACGCTGCTCCTATTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATTAAAG
CAAAAACTTATCGCTATATGGGTGCGGTTGAAAACCAAAATAATAGTGATCCTCAAGTGATAAATGGTGAAAGTCAGATA
AACGCCTCCGATAGCCGTAATGTACATATTCCTTCTATAAAGAAGTCAAAGCGAAAGTAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

89.732

86.486

0.776
  comO Acinetobacter baylyi ADP1

71.245

89.961

0.641

Multiple sequence alignment