Detailed information    

insolico Bioinformatically predicted

Overview

Name   comGA   Type   Machinery gene
Locus tag   LFL98_RS12515 Genome accession   NZ_CP110365
Coordinates   2419305..2420375 (-) Length   356 a.a.
NCBI ID   WP_181217184.1    Uniprot ID   -
Organism   Bacillus subtilis strain HY2-62     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2414305..2425375
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  LFL98_RS12470 (LFL98_12475) tapA 2414784..2415545 (-) 762 WP_015251716.1 amyloid fiber anchoring/assembly protein TapA -
  LFL98_RS12475 (LFL98_12480) yqzG 2415817..2416143 (+) 327 WP_003246051.1 YqzG/YhdC family protein -
  LFL98_RS12480 (LFL98_12485) spoIIT 2416185..2416364 (-) 180 WP_029726723.1 YqzE family protein -
  LFL98_RS12485 (LFL98_12490) comGG 2416435..2416809 (-) 375 WP_015251714.1 ComG operon protein ComGG Machinery gene
  LFL98_RS12490 (LFL98_12495) comGF 2416810..2417193 (-) 384 WP_015251713.1 ComG operon protein ComGF Machinery gene
  LFL98_RS12495 (LFL98_12500) comGE 2417219..2417566 (-) 348 WP_003230165.1 ComG operon protein 5 Machinery gene
  LFL98_RS12500 (LFL98_12505) comGD 2417550..2417981 (-) 432 WP_217011424.1 competence type IV pilus minor pilin ComGD Machinery gene
  LFL98_RS12505 (LFL98_12510) comGC 2417971..2418267 (-) 297 WP_003230162.1 comG operon protein ComGC Machinery gene
  LFL98_RS12510 (LFL98_12515) comGB 2418281..2419318 (-) 1038 WP_029317912.1 comG operon protein ComGB Machinery gene
  LFL98_RS12515 (LFL98_12520) comGA 2419305..2420375 (-) 1071 WP_181217184.1 competence protein ComGA Machinery gene
  LFL98_RS12520 (LFL98_12525) corA 2420778..2421731 (-) 954 WP_004399136.1 magnesium transporter CorA -
  LFL98_RS12525 (LFL98_12530) yqhB 2421874..2423202 (+) 1329 WP_014480261.1 hemolysin family protein -
  LFL98_RS12530 (LFL98_12535) rsbRD 2423255..2424091 (-) 837 WP_029317909.1 RsbT co-antagonist protein RsbRD -
  LFL98_RS12540 (LFL98_12545) mgsR 2424315..2424695 (-) 381 WP_003230147.1 transcriptional regulator MgsR -
  LFL98_RS12545 (LFL98_12550) yqgY 2424927..2425172 (+) 246 WP_003226300.1 DUF2626 domain-containing protein -

Sequence

Protein


Download         Length: 356 a.a.        Molecular weight: 40487.68 Da        Isoelectric Point: 9.1812

>NTDB_id=754631 LFL98_RS12515 WP_181217184.1 2419305..2420375(-) (comGA) [Bacillus subtilis strain HY2-62]
MDSIEKVSKNLIEEAYLTKASDIHIVPRERDAIIHFRVDHALLKKRDMKKEECVRLISHFKFLSAMDIGERRKPQNGSLT
LKLKEGNVHLRMSTLPTINEESLVIRVMPQYNIPSIDKLSLFPKTGATLLSFLKHSHGMLIFTGPTGSGKTTTLYSLVQY
AKKHFNRNIVTLEDPVETRDEDVLQVQVNEKAGVTYSTGLKAILRHDPDMIILGEIRDAETAEIAVRAAMTGHLVLTSLH
TRDAKGAIYRLLEFGINMNEIQQTVIAIAAQRLVDLACPFCENGCSSVYCRQSRNTRRASVYELLYGKNLQQCIQEAKGN
HANYQYQTLRQIIRKGIALGYLTTNNYDRWVYHEKD

Nucleotide


Download         Length: 1071 bp        

>NTDB_id=754631 LFL98_RS12515 WP_181217184.1 2419305..2420375(-) (comGA) [Bacillus subtilis strain HY2-62]
TTGGATTCAATAGAAAAGGTAAGCAAAAACTTGATTGAAGAGGCATATCTAACAAAGGCTTCTGATATTCACATCGTGCC
GAGGGAGCGGGACGCTATCATTCATTTTCGGGTCGATCATGCCTTGCTGAAAAAAAGGGACATGAAAAAAGAAGAGTGCG
TAAGACTGATTTCACATTTTAAATTTCTTTCAGCAATGGATATAGGTGAAAGGCGAAAGCCGCAAAACGGTTCGCTTACG
TTAAAGCTGAAAGAGGGAAATGTTCATTTAAGAATGTCAACGCTGCCCACAATTAATGAAGAAAGCCTCGTGATCAGAGT
GATGCCCCAATACAATATCCCTTCGATTGATAAATTGTCGCTATTTCCGAAGACAGGAGCCACATTACTCTCGTTTCTAA
AACATTCCCATGGCATGCTCATTTTTACCGGGCCAACTGGTTCAGGGAAGACTACCACACTATACTCTCTCGTTCAATAT
GCAAAAAAACACTTTAATCGAAATATTGTCACATTAGAGGACCCTGTTGAAACAAGGGACGAAGATGTTCTTCAGGTTCA
GGTGAATGAAAAAGCCGGTGTAACTTATTCCACAGGTCTGAAAGCAATTTTGCGCCATGATCCCGATATGATTATTTTAG
GTGAGATCAGAGACGCGGAAACAGCTGAAATTGCGGTGCGGGCAGCGATGACGGGACATCTGGTACTAACGAGCCTTCAT
ACGAGAGACGCAAAGGGCGCAATTTACAGACTGCTTGAATTCGGTATCAATATGAATGAAATCCAACAGACTGTCATTGC
AATAGCGGCTCAGCGCTTGGTTGACTTGGCTTGCCCGTTTTGTGAAAACGGATGTTCATCAGTGTATTGCCGACAGTCAC
GAAATACCAGGAGAGCTAGCGTTTATGAGCTTCTATACGGGAAAAATCTTCAGCAATGTATCCAGGAGGCAAAAGGAAAT
CATGCAAATTACCAATATCAAACGCTTCGTCAAATTATCAGAAAAGGAATTGCGCTCGGCTATTTAACGACAAACAACTA
TGACCGGTGGGTTTATCATGAAAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(10-275)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comGA Bacillus subtilis subsp. subtilis str. 168

99.438

100

0.994
  pilF Thermus thermophilus HB27

36.827

99.157

0.365
  pilB Glaesserella parasuis strain SC1401

43.878

82.584

0.362