Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   PALA52_RS23370 Genome accession   NZ_CP110347
Coordinates   5064651..5066243 (+) Length   530 a.a.
NCBI ID   WP_024914652.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA52     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5059651..5071243
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA52_RS23350 (PALA52_04636) pgeF 5061401..5062129 (-) 729 WP_024914656.1 peptidoglycan editing factor PgeF -
  PALA52_RS23355 (PALA52_04637) rluD 5062126..5063088 (-) 963 WP_024914655.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PALA52_RS23360 (PALA52_04638) - 5063234..5064259 (+) 1026 WP_024914654.1 outer membrane protein assembly factor BamD -
  PALA52_RS23365 (PALA52_04639) - 5064416..5064661 (+) 246 WP_024914653.1 PP0621 family protein -
  PALA52_RS23370 (PALA52_04640) pilS 5064651..5066243 (+) 1593 WP_024914652.1 two-component system sensor histidine kinase PilS Regulator
  PALA52_RS23375 (PALA52_04641) pilR 5066258..5067595 (+) 1338 WP_024914651.1 two-component system response regulator PilR Regulator
  PALA52_RS23380 (PALA52_04642) thiO 5067640..5068734 (-) 1095 WP_024914650.1 glycine oxidase ThiO -
  PALA52_RS23385 (PALA52_04643) - 5068869..5069378 (+) 510 WP_058141281.1 GspH/FimT family pseudopilin -
  PALA52_RS23390 (PALA52_04644) - 5069484..5069990 (+) 507 WP_003102598.1 GspH/FimT family pseudopilin -
  PALA52_RS23395 (PALA52_04645) pilV 5069981..5070538 (+) 558 WP_058141282.1 type 4a pilus minor pilin PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59066.17 Da        Isoelectric Point: 6.5043

>NTDB_id=754277 PALA52_RS23370 WP_024914652.1 5064651..5066243(+) (pilS) [Pseudomonas aeruginosa strain PALA52]
MRAERLRLSEEQGQRILRLYHLYRLTIGLVLVLLISSELEEQVLKLVHPELFHVGSWCYLVFNILVALFLPPSRQLLPIF
ILALTDVLMLCGLFYAGGGVPSGIGSLLVVAVAIANILLRGRIGLVIAAAASLGLLYLTFFLSLNSPDATNHYVQAGGLG
TLCFAAALVIQALVRRQEQTETLAEERAETVANLEELNALILQRMRTGILVVDSRQAILLANQAALGLLRQDDVQGASLS
HHSPMLLHCMKQWRLNPSLRPPTLKVVPDGPTVQPSFIGLNREDDQHVLIFLEDISQIAQQAQQMKLAGLGRLTAGIAHE
IRNPLGAISHAAQLLQESEELDAPDRRLTQIIQDQSKRMNLVIENVLQLSRRRQAEPQQLDLKEWLRRFVDEYPGRLRND
SELHLQLGDGDIQTRMDPHQLNQVLSNLVQNGLRYSAQAHGRGQVWLSLARDPESDLPVLEVIDDGPGVATDKLNNLFEP
FFTTESKGTGLGLYLSRELCESNQARIDYRNREEGGGCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=754277 PALA52_RS23370 WP_024914652.1 5064651..5066243(+) (pilS) [Pseudomonas aeruginosa strain PALA52]
GTGCGCGCTGAGCGGCTACGGCTGAGCGAGGAGCAGGGGCAACGCATCCTCCGCCTGTACCACCTGTACCGCCTGACCAT
CGGCCTGGTGCTGGTCCTGCTGATCTCCAGCGAGCTGGAAGAGCAGGTCCTCAAGCTCGTCCACCCTGAACTGTTCCATG
TCGGCAGCTGGTGCTACCTGGTCTTCAACATCCTCGTCGCGCTGTTCCTGCCTCCGTCACGGCAACTCCTGCCGATCTTC
ATCCTCGCCCTCACCGACGTGTTGATGCTTTGCGGCCTGTTCTACGCAGGTGGCGGCGTGCCCAGCGGCATCGGCAGCCT
GCTGGTGGTGGCGGTGGCCATTGCCAACATCCTGCTGCGCGGGCGCATCGGCCTGGTCATCGCGGCGGCTGCCAGCCTCG
GCCTGCTCTACCTGACCTTCTTCCTCAGCCTGAACAGTCCCGACGCCACCAACCACTACGTCCAGGCCGGCGGCCTCGGT
ACCCTGTGCTTCGCCGCCGCGCTGGTGATCCAGGCCCTGGTACGGCGCCAGGAGCAGACCGAAACGCTGGCCGAAGAACG
CGCCGAGACGGTCGCCAACCTGGAGGAGCTCAACGCATTGATCCTGCAGCGCATGCGCACCGGCATCCTCGTGGTCGACA
GCCGCCAGGCCATCCTCCTCGCCAACCAGGCCGCCCTCGGCCTGCTCAGGCAGGACGACGTACAGGGCGCCAGCCTCAGC
CATCACAGCCCGATGTTGCTGCACTGCATGAAGCAATGGCGCCTGAATCCCAGCCTCCGTCCGCCAACGCTCAAGGTGGT
GCCGGATGGCCCGACGGTGCAACCCAGCTTTATCGGCCTCAACCGCGAAGACGACCAGCACGTGCTGATCTTCCTCGAAG
ACATTTCGCAGATCGCCCAGCAGGCGCAGCAGATGAAGCTGGCCGGACTCGGCCGCCTGACCGCCGGCATCGCCCATGAG
ATCCGCAACCCGCTGGGCGCGATCAGCCACGCCGCCCAACTGCTGCAAGAGTCCGAGGAACTGGATGCCCCCGACCGGCG
CCTGACGCAGATCATCCAGGACCAGTCGAAGCGGATGAACCTGGTCATCGAGAACGTCCTGCAGCTCTCCCGTCGCCGCC
AGGCCGAACCGCAGCAGCTCGACCTGAAGGAGTGGCTGCGGCGGTTCGTCGACGAATACCCCGGCAGGCTGCGCAACGAC
AGCGAACTGCACCTGCAACTCGGTGACGGCGACATCCAGACCCGCATGGACCCACATCAGTTGAACCAGGTGCTGAGCAA
CCTGGTGCAGAACGGTCTTCGCTACAGCGCCCAGGCGCACGGGCGCGGCCAGGTCTGGCTGAGCCTCGCGCGCGACCCGG
AGAGCGACCTGCCGGTGCTGGAAGTCATCGACGACGGTCCCGGCGTAGCGACGGACAAACTGAACAACCTGTTCGAACCC
TTCTTTACTACAGAAAGCAAAGGTACCGGCCTGGGCCTCTATCTCTCCCGCGAACTCTGCGAGAGCAACCAGGCACGGAT
CGACTACCGCAATCGCGAGGAAGGCGGCGGCTGCTTCCGCATCACCTTCGCCCACCCGCGCAAACTCAGCTGA

Domains

Predicted by InterproScan.

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

His Kinase A (phospho-acceptor) domain (HisKA)

(310-373)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

97.925

100

0.979