Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   UKS_RS09515 Genome accession   NZ_AP021887
Coordinates   1909791..1911089 (-) Length   432 a.a.
NCBI ID   WP_156012964.1    Uniprot ID   A0A6H3SNE7
Organism   Streptococcus sp. 116-D4     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1904791..1916089
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  UKS_RS09495 (UKS_18420) rplI 1905945..1906397 (-) 453 WP_156012956.1 50S ribosomal protein L9 -
  UKS_RS09500 (UKS_18430) - 1906394..1908367 (-) 1974 WP_156012958.1 DHH family phosphoesterase -
  UKS_RS09505 (UKS_18440) raiA 1908504..1909052 (-) 549 WP_156012960.1 ribosome-associated translation inhibitor RaiA -
  UKS_RS09510 (UKS_18450) comFC/cflB 1909132..1909794 (-) 663 WP_156012962.1 ComF family protein Machinery gene
  UKS_RS09515 (UKS_18460) comFA/cflA 1909791..1911089 (-) 1299 WP_156012964.1 DEAD/DEAH box helicase Machinery gene
  UKS_RS09520 (UKS_18470) - 1911146..1911781 (+) 636 WP_156012966.1 YigZ family protein -
  UKS_RS09525 (UKS_18480) - 1911797..1912240 (+) 444 WP_156012968.1 PH domain-containing protein -
  UKS_RS09530 (UKS_18490) cysK 1912337..1913263 (+) 927 WP_117304672.1 cysteine synthase A -
  UKS_RS09535 (UKS_18500) - 1913484..1914740 (+) 1257 WP_156012970.1 ISL3 family transposase -
  UKS_RS09540 (UKS_18510) tsf 1914807..1915847 (-) 1041 WP_049494714.1 translation elongation factor Ts -

Sequence

Protein


Download         Length: 432 a.a.        Molecular weight: 49557.40 Da        Isoelectric Point: 7.9804

>NTDB_id=75391 UKS_RS09515 WP_156012964.1 1909791..1911089(-) (comFA/cflA) [Streptococcus sp. 116-D4]
MKINPNYIGRLFTENELSEEDRQLAEKLPAMRKEKGKLFCQRCNSPILEEWSLPIGAYYCRECLLMKRVRSDQALYYFPQ
EDFPKQDVLKWCGQLTPFQEKVSEGLLQAVEKQEPTLVHAVTGAGKTEMIYQVVAKVIDKGGAVCLASPRIDVCLELYKR
LQDDFACDISLLHGESDPYFRTPLVVATTHQLLKFYQAFDLLIVDEVDAFPYVDNPTLYYAVKNSVKENGLRIFLTATST
DELDRKVRLGELKRLSLPRRFHGNPLIIPKPVWLSDFNHCLEKNRLSPKLKSYIEKQRKTAYPLLIFASEIKKGEQLKEI
LQEQFPNEKIGFVSSITENRLEQVQAFRDRELTILISTTILERGVTFPCVDVFVVEANHRLFTKSSLIQIGGRVGRSMDR
PTGDLLFFHDGINASIKKAIKEIQQMNKEAGL

Nucleotide


Download         Length: 1299 bp        

>NTDB_id=75391 UKS_RS09515 WP_156012964.1 1909791..1911089(-) (comFA/cflA) [Streptococcus sp. 116-D4]
ATGAAAATAAATCCAAATTATATCGGTCGTTTGTTTACGGAGAATGAATTATCAGAAGAGGATCGTCAGTTGGCGGAGAA
ACTTCCAGCAATGAGAAAGGAGAAAGGGAAACTTTTCTGTCAACGTTGTAATAGTCCTATTCTAGAAGAATGGTCTTTAC
CCATCGGTGCTTACTATTGTAGGGAGTGTTTGCTGATGAAGCGAGTCAGGAGTGATCAAGCTTTATACTATTTTCCGCAG
GAGGATTTTCCGAAGCAAGATGTTCTTAAATGGTGCGGTCAATTAACTCCTTTTCAAGAAAAAGTATCAGAAGGATTGCT
TCAAGCGGTAGAAAAGCAAGAGCCAACCTTGGTTCACGCTGTAACAGGAGCTGGAAAGACAGAGATGATTTACCAAGTTG
TGGCCAAAGTGATTGATAAAGGTGGTGCAGTTTGTTTGGCCAGCCCTAGAATTGATGTGTGTTTGGAACTGTATAAACGA
TTGCAAGATGATTTTGCTTGCGATATATCTTTACTACACGGAGAATCGGATCCCTATTTTCGTACACCTTTAGTTGTAGC
AACCACTCACCAACTATTAAAGTTTTATCAAGCTTTTGATTTGCTGATAGTGGATGAAGTAGATGCCTTTCCTTATGTTG
ACAATCCTACGCTTTACTACGCTGTCAAGAATAGTGTAAAGGAAAATGGCTTGAGAATTTTTTTAACAGCGACTTCTACC
GATGAGTTAGATAGGAAGGTCCGTTTAGGGGAATTAAAACGATTGAGCTTGCCAAGACGATTCCATGGAAATCCTTTGAT
TATTCCTAAGCCTGTCTGGTTATCGGATTTTAATCACTGTTTAGAGAAAAATAGGTTGTCACCAAAGTTAAAGTCCTATA
TTGAGAAGCAGAGAAAGACAGCTTATCCGTTACTCATTTTTGCTTCAGAGATTAAGAAAGGGGAACAGTTAAAAGAAATC
TTACAGGAGCAATTTCCAAACGAGAAAATTGGCTTTGTATCTTCTATCACAGAAAATCGATTAGAGCAGGTACAAGCATT
TCGAGATAGAGAACTGACAATACTTATCAGTACGACGATATTGGAGCGTGGAGTTACCTTCCCTTGTGTTGACGTTTTCG
TAGTAGAGGCCAATCATCGACTGTTTACAAAGTCTAGCTTGATTCAGATTGGTGGACGAGTTGGGCGAAGCATGGATAGA
CCGACAGGAGATTTGCTTTTCTTTCATGATGGGATAAATGCTTCAATCAAGAAGGCAATTAAGGAAATTCAGCAGATGAA
TAAGGAGGCGGGTCTATGA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(94-168)

Helicase conserved C-terminal domain (Helicase_C)

(293-396)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6H3SNE7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

93.75

100

0.938
  comFA/cflA Streptococcus pneumoniae Rx1

92.13

100

0.921
  comFA/cflA Streptococcus pneumoniae D39

92.13

100

0.921
  comFA/cflA Streptococcus pneumoniae R6

92.13

100

0.921
  comFA/cflA Streptococcus pneumoniae TIGR4

91.898

100

0.919
  comFA/cflA Streptococcus mitis SK321

91.204

100

0.912
  comFA Lactococcus lactis subsp. cremoris KW2

50.583

99.306

0.502
  comFA Latilactobacillus sakei subsp. sakei 23K

38.215

100

0.387

Multiple sequence alignment