Detailed information    

insolico Bioinformatically predicted

Overview

Name   comFA/cflA   Type   Machinery gene
Locus tag   NOV96_RS10210 Genome accession   NZ_CP110141
Coordinates   2078338..2079630 (-) Length   430 a.a.
NCBI ID   WP_004194121.1    Uniprot ID   A0A140EWW0
Organism   Streptococcus suis strain WUSS030     
Function   ssDNA transport into the cell (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2073338..2084630
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NOV96_RS10200 (NOV96_10200) hpf 2077061..2077603 (-) 543 WP_004194117.1 ribosome hibernation-promoting factor, HPF/YfiA family -
  NOV96_RS10205 (NOV96_10205) comFC/cflB 2077680..2078345 (-) 666 WP_004194119.1 ComF family protein Machinery gene
  NOV96_RS10210 (NOV96_10210) comFA/cflA 2078338..2079630 (-) 1293 WP_004194121.1 DEAD/DEAH box helicase Machinery gene
  NOV96_RS10215 (NOV96_10215) - 2079687..2080319 (+) 633 WP_004194123.1 YigZ family protein -
  NOV96_RS10220 (NOV96_10220) - 2080770..2081970 (+) 1201 Protein_1969 IS110 family transposase -
  NOV96_RS10225 (NOV96_10225) - 2082285..2082749 (-) 465 WP_014636659.1 PBECR2 nuclease fold domain-containing protein -
  NOV96_RS10230 (NOV96_10230) - 2082727..2083464 (-) 738 WP_024417665.1 PlySs2 family phage lysin -
  NOV96_RS10235 (NOV96_10235) - 2083448..2083798 (-) 351 WP_002937841.1 phage holin -
  NOV96_RS10240 (NOV96_10240) - 2083801..2084100 (-) 300 WP_014636661.1 hypothetical protein -
  NOV96_RS10245 (NOV96_10245) - 2084104..2084448 (-) 345 WP_002936845.1 hypothetical protein -

Sequence

Protein


Download         Length: 430 a.a.        Molecular weight: 48868.45 Da        Isoelectric Point: 9.2762

>NTDB_id=752681 NOV96_RS10210 WP_004194121.1 2078338..2079630(-) (comFA/cflA) [Streptococcus suis strain WUSS030]
MKELENYYGRLFTKYQLTAKEREIAEKVPSITKKNNCFRCGTTFKEENKLPNDAYYCRACLLLGRVRSDEKLYHFPQKDF
PITKCLKWKGQLTDWQQRISDGLVANVENNRATLVHAVTGAGKTEMIYHTVASVIDKGGAVCLASPRIDVCIELYKRLQN
DFSVPISLLHGESEPYFRTPLVVATTHQLLKFYQAFDLVLIDEVDAFPYADNPMLYQAADNAVKEAGVQVFLTATSTDEL
DKKVRTGKLSRLSLPRRFHGNPLVVPQKVWFSKFDDTLKKNRLVPKLKKAIEEQRKSGFPLLIFVPEISKGQEFTKIMKK
TFPEETIGFVSSQTENRLEIVEGFRKREITVLISTTILERGVTFPCVDVFVVQANHYLYTASSLVQIAGRVGRSIERPTG
LLQFYHEGSTGAIEKAIAEIKQMNKEAGYV

Nucleotide


Download         Length: 1293 bp        

>NTDB_id=752681 NOV96_RS10210 WP_004194121.1 2078338..2079630(-) (comFA/cflA) [Streptococcus suis strain WUSS030]
ATGAAAGAATTAGAAAATTATTATGGAAGATTATTTACCAAATACCAATTGACAGCAAAAGAAAGAGAAATAGCAGAAAA
AGTGCCAAGTATTACAAAAAAGAATAACTGCTTTCGCTGTGGAACAACTTTTAAAGAAGAAAACAAATTGCCAAACGATG
CTTATTACTGTCGAGCCTGCTTGCTTCTAGGCAGAGTACGGTCAGACGAAAAACTCTATCATTTTCCTCAGAAAGATTTT
CCAATCACTAAGTGTTTAAAGTGGAAAGGTCAACTAACTGATTGGCAACAAAGAATTTCAGATGGACTAGTTGCAAACGT
GGAAAATAATCGTGCGACATTGGTTCATGCAGTAACAGGAGCAGGTAAGACAGAAATGATCTACCACACCGTTGCCTCAG
TGATTGATAAAGGCGGAGCGGTTTGCCTAGCCAGTCCTCGAATTGATGTTTGTATCGAACTCTATAAACGTCTGCAAAAT
GACTTTTCAGTTCCAATTAGTTTACTACATGGAGAGTCTGAACCCTATTTCCGAACCCCATTAGTTGTAGCAACCACACA
TCAGTTATTAAAATTTTATCAGGCCTTTGATTTGGTTTTGATTGATGAAGTAGACGCCTTTCCCTATGCAGATAATCCCA
TGCTCTATCAAGCAGCAGACAATGCGGTCAAGGAAGCCGGTGTTCAAGTTTTTCTGACAGCGACTTCAACAGATGAATTG
GATAAAAAAGTCAGAACAGGTAAATTAAGTCGTCTTAGTTTGCCAAGGCGCTTTCATGGCAACCCACTTGTTGTCCCGCA
AAAAGTCTGGTTTAGTAAATTCGATGATACCCTAAAGAAAAATAGACTAGTCCCAAAGTTGAAAAAAGCGATTGAAGAAC
AGAGAAAGTCGGGCTTTCCCTTACTCATTTTTGTCCCAGAAATCTCCAAAGGTCAAGAATTTACCAAGATAATGAAAAAA
ACATTCCCAGAAGAAACAATTGGCTTTGTATCCAGTCAAACAGAAAATCGCCTTGAAATAGTTGAAGGGTTTCGCAAGAG
AGAAATCACAGTCTTAATCTCGACTACTATTCTTGAACGTGGGGTGACCTTCCCATGTGTAGACGTCTTTGTTGTTCAAG
CTAATCATTACCTCTACACAGCGTCAAGTCTTGTTCAGATTGCAGGCCGGGTCGGAAGGAGTATAGAACGTCCGACTGGT
TTACTTCAGTTTTATCATGAGGGAAGTACAGGAGCCATTGAAAAGGCAATCGCTGAAATTAAACAGATGAACAAGGAGGC
TGGTTATGTCTAA

Domains

Predicted by InterproScan.

Type III restriction enzyme, res subunit (ResIII)

(91-235)

Helicase conserved C-terminal domain (Helicase_C)

(289-393)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A140EWW0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comFA/cflA Streptococcus mitis NCTC 12261

67.053

100

0.672
  comFA/cflA Streptococcus pneumoniae Rx1

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae D39

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae R6

66.357

100

0.665
  comFA/cflA Streptococcus pneumoniae TIGR4

66.357

100

0.665
  comFA/cflA Streptococcus mitis SK321

65.893

100

0.66
  comFA Lactococcus lactis subsp. cremoris KW2

54.156

92.326

0.5
  comFA Latilactobacillus sakei subsp. sakei 23K

38.051

100

0.381
  comFA Bacillus subtilis subsp. subtilis str. 168

37.59

96.512

0.363