Detailed information    

insolico Bioinformatically predicted

Overview

Name   radA   Type   Machinery gene
Locus tag   OLM03_RS00180 Genome accession   NZ_CP110039
Coordinates   42764..44134 (+) Length   456 a.a.
NCBI ID   WP_002356050.1    Uniprot ID   -
Organism   Enterococcus faecalis strain BE47     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 37764..49134
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OLM03_RS00155 (OLM03_00155) - 38254..38721 (-) 468 WP_016630662.1 VOC family protein -
  OLM03_RS00160 (OLM03_00160) - 38786..39733 (+) 948 WP_016630661.1 YafY family protein -
  OLM03_RS00165 (OLM03_00165) - 39825..41078 (-) 1254 WP_016630660.1 glutamate-5-semialdehyde dehydrogenase -
  OLM03_RS00170 (OLM03_00170) proB 41065..41880 (-) 816 WP_002389550.1 glutamate 5-kinase -
  OLM03_RS00175 (OLM03_00175) - 42200..42685 (+) 486 WP_002367569.1 dUTP diphosphatase -
  OLM03_RS00180 (OLM03_00180) radA 42764..44134 (+) 1371 WP_002356050.1 DNA repair protein RadA Machinery gene
  OLM03_RS00185 (OLM03_00185) - 44235..45380 (+) 1146 WP_002368622.1 PIN/TRAM domain-containing protein -
  OLM03_RS00190 (OLM03_00190) ispF 45399..45872 (+) 474 WP_002367570.1 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase -
  OLM03_RS00195 (OLM03_00195) gltX 45925..47382 (+) 1458 WP_010709111.1 glutamate--tRNA ligase -
  OLM03_RS00200 (OLM03_00200) epsC 47652..48191 (+) 540 WP_002356054.1 serine O-acetyltransferase EpsC -

Sequence

Protein


Download         Length: 456 a.a.        Molecular weight: 49757.28 Da        Isoelectric Point: 7.3489

>NTDB_id=751618 OLM03_RS00180 WP_002356050.1 42764..44134(+) (radA) [Enterococcus faecalis strain BE47]
MAKKAKVQFECQSCGYVSPKYLGRCPNCGQWNSMVEEVIQDTSDRRARVSLTGKKTQPQRLSEVVPKKEPRVKTELVELN
RVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSQQLAATGGTVLYVSGEESAEQIKLRAERLGTVNETFYLYAETDMHEISR
AIEKLEPDYVIIDSIQTMTQPDVTSVAGSVSQVRETTAELLKLAKTNGIAIFIVGHVTKEGSIAGPRMLEHMVDTVLYFE
GDKHHTFRILRAVKNRFGSTNEIGIFEMQTHGLVEVMNPSQVFLEERLEGATGSSIVVAMEGSRPILVEIQALVTPTMFG
NAKRTTTGLDFNRVSLIMAVLEKRAGLLLQNQDAYLKAAGGVKINEPAIDLALAISIASSYKEKGTSSSECFIGEIGLTG
EIRRVNSIEQRVREAQKLGFTKVYVPKNNLGGWEAPEGIEIIGVSTIGETLRKVFK

Nucleotide


Download         Length: 1371 bp        

>NTDB_id=751618 OLM03_RS00180 WP_002356050.1 42764..44134(+) (radA) [Enterococcus faecalis strain BE47]
ATGGCAAAAAAAGCAAAAGTTCAATTTGAATGTCAAAGCTGTGGGTATGTGTCTCCAAAATATCTCGGCCGCTGTCCTAA
TTGTGGACAATGGAATTCGATGGTGGAAGAGGTTATTCAAGATACTTCTGATCGTCGTGCTAGAGTAAGTTTGACTGGTA
AAAAGACACAACCACAACGGCTATCTGAAGTCGTACCTAAAAAAGAGCCTCGTGTAAAAACTGAATTAGTGGAGCTGAAT
CGGGTTTTAGGTGGTGGCGTTGTTCCAGGTTCGTTAGTTTTAATCGGTGGCGATCCCGGAATAGGTAAGTCAACATTGCT
CTTACAAGTATCGCAACAATTAGCAGCCACAGGTGGCACGGTTTTATATGTTTCTGGTGAAGAAAGTGCTGAGCAAATTA
AATTGCGAGCGGAGCGTTTAGGCACCGTCAACGAGACATTTTACTTATACGCAGAAACAGATATGCACGAAATATCACGA
GCCATTGAAAAATTAGAACCAGACTATGTCATTATTGACTCGATTCAAACAATGACGCAGCCAGATGTTACCAGCGTTGC
TGGTAGTGTCAGTCAAGTCCGAGAAACAACTGCTGAATTATTGAAACTGGCAAAAACGAATGGGATTGCCATTTTTATTG
TCGGACACGTAACGAAGGAAGGGTCTATCGCAGGGCCACGGATGTTGGAACATATGGTAGACACGGTTCTTTATTTTGAA
GGAGATAAGCATCACACCTTTAGAATCTTACGGGCTGTCAAAAATCGGTTTGGCTCAACGAATGAGATTGGTATTTTTGA
AATGCAGACACATGGATTGGTTGAAGTTATGAATCCTTCTCAGGTCTTTTTAGAAGAACGTTTAGAAGGAGCCACCGGAT
CTTCAATTGTGGTAGCAATGGAAGGTTCACGCCCCATTTTGGTGGAAATCCAAGCGTTAGTTACTCCAACGATGTTTGGC
AATGCCAAGCGAACGACCACCGGGTTGGACTTTAATCGCGTTTCTTTAATCATGGCGGTTTTAGAAAAACGAGCAGGCCT
TTTGTTACAAAACCAGGATGCGTATCTAAAAGCGGCTGGTGGAGTCAAAATTAATGAACCAGCTATTGATTTAGCGTTAG
CAATCAGTATTGCTTCTAGCTATAAAGAAAAAGGAACCTCATCTTCGGAATGTTTCATTGGCGAGATTGGTTTAACTGGT
GAAATTCGCCGCGTGAATAGTATTGAACAACGAGTGCGTGAAGCGCAAAAACTAGGCTTTACAAAAGTCTATGTCCCTAA
AAATAACCTTGGTGGCTGGGAAGCGCCTGAAGGTATTGAAATTATTGGCGTTTCAACAATTGGTGAAACGTTAAGAAAAG
TTTTTAAATAA

Domains

Predicted by InterproScan.

AAA domain (AAA_25)

(65-221)

Subunit ChlI of Mg-chelatase (ChlI)

(362-429)

Rubredoxin metal binding domain (Zn_ribbon_LapB)

(9-36)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  radA Streptococcus mitis NCTC 12261

75.385

99.781

0.752
  radA Streptococcus pneumoniae Rx1

75.385

99.781

0.752
  radA Streptococcus pneumoniae D39

75.385

99.781

0.752
  radA Streptococcus pneumoniae R6

75.385

99.781

0.752
  radA Streptococcus pneumoniae TIGR4

75.385

99.781

0.752
  radA Streptococcus mitis SK321

75.385

99.781

0.752
  radA Bacillus subtilis subsp. subtilis str. 168

66.297

98.904

0.656