Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilR   Type   Regulator
Locus tag   PALA32_RS24995 Genome accession   NZ_CP109844
Coordinates   5365299..5366636 (+) Length   445 a.a.
NCBI ID   WP_023088571.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain PALA32     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 5360299..5371636
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PALA32_RS24970 (PALA32_04942) pgeF 5360442..5361170 (-) 729 WP_003102588.1 peptidoglycan editing factor PgeF -
  PALA32_RS24975 (PALA32_04943) rluD 5361167..5362129 (-) 963 WP_003094686.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  PALA32_RS24980 (PALA32_04944) - 5362275..5363300 (+) 1026 WP_003102590.1 outer membrane protein assembly factor BamD -
  PALA32_RS24985 (PALA32_04945) - 5363457..5363702 (+) 246 WP_003102591.1 PP0621 family protein -
  PALA32_RS24990 (PALA32_04946) pilS 5363692..5365284 (+) 1593 WP_003094692.1 two-component system sensor histidine kinase PilS Regulator
  PALA32_RS24995 (PALA32_04947) pilR 5365299..5366636 (+) 1338 WP_023088571.1 two-component system response regulator PilR Regulator
  PALA32_RS25000 (PALA32_04948) thiO 5366682..5367776 (-) 1095 WP_023084775.1 glycine oxidase ThiO -
  PALA32_RS25005 (PALA32_04949) - 5367911..5368420 (+) 510 WP_023088572.1 GspH/FimT family pseudopilin -
  PALA32_RS25010 (PALA32_04950) - 5368526..5369032 (+) 507 WP_003102598.1 GspH/FimT family pseudopilin -
  PALA32_RS25015 (PALA32_04951) pilV 5369023..5369580 (+) 558 WP_003119423.1 type 4a pilus minor pilin PilV Machinery gene
  PALA32_RS25020 (PALA32_04952) pilW 5369577..5370401 (+) 825 WP_003119422.1 type 4a pilus minor pilin PilW -
  PALA32_RS25025 (PALA32_04953) pilX 5370398..5370985 (+) 588 WP_003102605.1 type 4a pilus minor pilin PilX -

Sequence

Protein


Download         Length: 445 a.a.        Molecular weight: 49755.09 Da        Isoelectric Point: 5.8654

>NTDB_id=750000 PALA32_RS24995 WP_023088571.1 5365299..5366636(+) (pilR) [Pseudomonas aeruginosa strain PALA32]
MSRQKALIVDDEPDIRELLEITLGRMKLDTRSARNVKEARELLAREPFDMCLTDMRLPDGSGLDLVQYIQQRHPQTPVAM
ITAYGSLDTAIQALKAGAFDFLTKPVDLGRLRELVATALRLRNPEAEEAPVDNRLLGESPPMRALRNQIGKLARSQAPVY
ISGESGSGKELVARLIHEQGPRIERPFVPVNCGAIPSELMESEFFGHKKGSFTGAIEDKQGLFQAASGGTLFLDEVADLP
MAMQVKLLRAIQEKAVRAVGGQQEVAVDVRILCATHKDLAAEVGAGRFRQDLYYRLNVIELRVPPLRERREDIPLLAERI
LKRLAGDTGLPAARLTGDAQEKLKNYRFPGNVRELENMLERAYTLCEDDQIQPHDLRLADAPGASQEGAASLSEIDNLED
YLEDIERKLIMQALEETRWNRTAAAQRLGLTFRSMRYRLKKLGID

Nucleotide


Download         Length: 1338 bp        

>NTDB_id=750000 PALA32_RS24995 WP_023088571.1 5365299..5366636(+) (pilR) [Pseudomonas aeruginosa strain PALA32]
ATGAGCCGACAAAAAGCCCTGATCGTCGACGATGAACCGGATATCCGCGAACTGCTGGAAATCACTCTCGGCCGCATGAA
GCTGGACACCCGCAGCGCCCGCAACGTCAAGGAAGCCCGCGAGTTGCTGGCCCGCGAGCCGTTCGACATGTGCCTCACCG
ACATGCGCCTGCCGGACGGCAGCGGCCTCGATCTGGTCCAGTACATCCAGCAGCGCCATCCACAGACCCCGGTGGCCATG
ATCACCGCGTACGGCAGCCTGGACACCGCGATCCAGGCGCTCAAGGCCGGTGCCTTCGACTTCCTCACCAAACCGGTCGA
CCTCGGTCGCTTGCGGGAGCTGGTGGCAACCGCCCTGCGCTTGCGCAACCCGGAAGCCGAGGAAGCGCCGGTGGACAACC
GCCTGCTCGGCGAGTCGCCGCCGATGCGCGCCCTGCGCAACCAGATCGGCAAGTTGGCGCGCAGCCAGGCGCCGGTCTAC
ATCAGTGGCGAGTCCGGCAGCGGCAAGGAACTGGTGGCGCGCCTGATCCACGAGCAGGGGCCACGTATCGAGCGGCCGTT
CGTGCCGGTGAACTGCGGCGCGATTCCCTCCGAACTGATGGAAAGCGAGTTCTTCGGCCACAAGAAAGGCAGCTTCACTG
GCGCTATCGAAGACAAGCAGGGCCTGTTCCAGGCCGCCAGCGGCGGCACCCTGTTCCTCGACGAAGTCGCCGACCTGCCG
ATGGCCATGCAGGTCAAACTGCTCCGGGCGATCCAGGAAAAGGCCGTGCGCGCGGTCGGCGGCCAGCAGGAGGTCGCCGT
CGACGTGCGCATCCTCTGCGCCACCCACAAGGACCTCGCCGCCGAAGTCGGCGCCGGGCGCTTCCGCCAGGACCTCTACT
ACCGCCTCAACGTCATCGAGCTGCGCGTACCGCCGCTGCGCGAACGCCGCGAGGACATCCCGCTGCTCGCCGAACGCATC
CTCAAGCGCCTGGCCGGCGACACCGGCCTGCCGGCCGCCAGGCTGACCGGCGACGCACAGGAGAAGCTGAAGAACTACCG
CTTCCCGGGCAACGTCCGCGAGCTGGAAAACATGCTGGAGCGCGCCTACACCCTGTGCGAAGACGACCAGATCCAGCCTC
ACGACCTGCGCCTGGCCGATGCGCCGGGTGCCAGCCAGGAAGGCGCCGCGAGCCTGAGCGAAATAGACAACCTCGAGGAC
TACCTGGAAGACATCGAGCGCAAGCTGATCATGCAGGCGCTCGAGGAGACCCGCTGGAACCGCACCGCCGCGGCTCAGCG
TCTGGGCCTGACGTTCCGCTCGATGCGCTACCGCCTGAAAAAGCTGGGCATCGACTGA

Domains

Predicted by InterproScan.

Response regulator receiver domain (Response_reg)

(7-115)

Sigma-54 interaction domain (Sigma54_activat)

(135-301)

Bacterial regulatory protein, Fis family (HTH_8)

(402-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilR Pseudomonas aeruginosa PAK

99.775

100

0.998
  pilR Acinetobacter baumannii strain A118

51.198

100

0.528
  luxO Vibrio cholerae strain A1552

37.946

100

0.382