Detailed information    

insolico Bioinformatically predicted

Overview

Name   nucA/comI   Type   Machinery gene
Locus tag   OHU75_RS03690 Genome accession   NZ_CP109761
Coordinates   675156..675578 (+) Length   140 a.a.
NCBI ID   WP_013057949.1    Uniprot ID   A0A8D3X0F8
Organism   Priestia megaterium strain P-NA14     
Function   cleavage of dsDNA into ssDNA (predicted from homology)   
DNA processing

Genomic Context

Location: 670156..680578
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OHU75_RS03665 (OHU75_03665) - 670487..671320 (-) 834 WP_264388136.1 AraC family transcriptional regulator -
  OHU75_RS03670 (OHU75_03670) - 671543..672364 (+) 822 WP_028409293.1 sensor histidine kinase -
  OHU75_RS03675 (OHU75_03675) - 672365..673006 (+) 642 WP_014459245.1 response regulator transcription factor -
  OHU75_RS03680 (OHU75_03680) - 673142..674521 (+) 1380 WP_098659027.1 PepSY domain-containing protein -
  OHU75_RS03685 (OHU75_03685) - 674551..675015 (+) 465 WP_098432233.1 FixH family protein -
  OHU75_RS03690 (OHU75_03690) nucA/comI 675156..675578 (+) 423 WP_013057949.1 NucA/NucB deoxyribonuclease domain-containing protein Machinery gene
  OHU75_RS03695 (OHU75_03695) - 675719..676072 (+) 354 WP_225049451.1 DUF1516 family protein -
  OHU75_RS03700 (OHU75_03700) - 676113..676706 (-) 594 WP_028409296.1 hypothetical protein -
  OHU75_RS03705 (OHU75_03705) - 676861..677517 (-) 657 WP_098580137.1 TetR/AcrR family transcriptional regulator -
  OHU75_RS03710 (OHU75_03710) - 677540..678820 (-) 1281 WP_264388137.1 glycosyltransferase -
  OHU75_RS03715 (OHU75_03715) - 679173..679394 (-) 222 WP_264388138.1 hypothetical protein -

Sequence

Protein


Download         Length: 140 a.a.        Molecular weight: 15329.17 Da        Isoelectric Point: 4.4990

>NTDB_id=749031 OHU75_RS03690 WP_013057949.1 675156..675578(+) (nucA/comI) [Priestia megaterium strain P-NA14]
MKKIVSGILILVLAVIVMIEGNPLEQNSSTNTSYDEVITFPADRYPETAAHIQHAIDKGKSAVCTIDRDGAEENRRESLK
GIPTKSGYDRDEFPMAFCEEGGSGADIEYVKPSDNRGAGSWISHQVDEFPDGTKVLIEVK

Nucleotide


Download         Length: 423 bp        

>NTDB_id=749031 OHU75_RS03690 WP_013057949.1 675156..675578(+) (nucA/comI) [Priestia megaterium strain P-NA14]
TTGAAAAAAATTGTTTCTGGTATTTTAATTTTAGTATTAGCCGTTATTGTTATGATTGAAGGAAATCCGTTAGAGCAGAA
CTCTTCAACGAATACCTCCTACGATGAAGTAATTACCTTCCCTGCTGATCGTTACCCTGAAACTGCAGCGCACATTCAAC
ATGCAATCGATAAAGGAAAATCGGCTGTTTGTACAATTGACCGAGACGGAGCTGAGGAAAACCGCCGTGAGTCTTTAAAA
GGTATTCCGACTAAAAGCGGCTATGACCGAGATGAGTTTCCGATGGCCTTTTGTGAAGAAGGCGGAAGCGGTGCTGATAT
TGAATATGTAAAGCCTTCAGACAACCGAGGCGCCGGGTCTTGGATCTCCCATCAAGTAGACGAGTTTCCTGACGGTACGA
AGGTTTTAATTGAAGTGAAGTAA

Domains

Predicted by InterproScan.

Deoxyribonuclease NucA/NucB (DNase_NucA_NucB)

(43-137)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  nucA/comI Bacillus subtilis subsp. subtilis str. 168

61.207

82.857

0.507