Detailed information    

insolico Bioinformatically predicted

Overview

Name   recG   Type   Machinery gene
Locus tag   QDW53_RS00375 Genome accession   NZ_CP122679
Coordinates   84478..86559 (-) Length   693 a.a.
NCBI ID   WP_001560130.1    Uniprot ID   -
Organism   Escherichia coli strain ETEC4069     
Function   homologous recombination (predicted from homology)   
Homologous recombination

Genomic Context

Location: 79478..91559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QDW53_RS00360 (QDW53_00360) xanP 80749..82140 (-) 1392 WP_001295238.1 xanthine/proton symporter XanP -
  QDW53_RS00365 (QDW53_00365) gltS 82419..83624 (+) 1206 WP_000468836.1 sodium/glutamate symporter -
  QDW53_RS00370 (QDW53_00370) - 83627..84493 (+) 867 WP_063086436.1 hypothetical protein -
  QDW53_RS00375 (QDW53_00375) recG 84478..86559 (-) 2082 WP_001560130.1 ATP-dependent DNA helicase RecG Machinery gene
  QDW53_RS00380 (QDW53_00380) trmH 86565..87254 (-) 690 WP_001070177.1 tRNA (guanosine(18)-2'-O)-methyltransferase TrmH -
  QDW53_RS00385 (QDW53_00385) spoT 87261..89369 (-) 2109 WP_000280488.1 bifunctional GTP diphosphokinase/guanosine-3',5'-bis pyrophosphate 3'-pyrophosphohydrolase -
  QDW53_RS00390 (QDW53_00390) rpoZ 89388..89663 (-) 276 WP_000135058.1 DNA-directed RNA polymerase subunit omega -
  QDW53_RS00395 (QDW53_00395) gmk 89718..90341 (-) 624 WP_001366734.1 guanylate kinase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 76314.28 Da        Isoelectric Point: 7.3295

>NTDB_id=742232 QDW53_RS00375 WP_001560130.1 84478..86559(-) (recG) [Escherichia coli strain ETEC4069]
MKGRLLDAVPLSSLTGVGAALSNKLAKINLHTVQDLLLHLPLRYEDRTHLYPIGELLPGVYATVEGEVLNCNISFGGRRM
MTCQISDGSGILTMRFFNFSAAMKNSLATGRRVLAYGEAKRGKYGAEMIHPEYRVQGDLSTPELQETLTPVYPTTEGVKQ
ATLRKLTDQALDLLDTCAIEELLPPELSQGMMTLPEALRTLHRPPPTLQLSDLETGQHPAQRRLILEELLAHNLSMLALR
AGAQRFHAQPLSANDALKNKLLAALPFKPTGAQARVVAEIERDMALDAPMMRLVQGDVGSGKTLVAALAALRAIAHGKQV
ALMAPTELLAEQHANNFRNWFAPLGIEVGWLAGKQKGKARLAQQEAIASGQVQMIVGTHAIFQEQVQFNGLALVIIDEQH
RFGVHQRLALWEKGQQQGFHPHQLIMTATPIPRTLAMTAYADLDTSVIDELPPGRTPVTTVAIPDTRRTDIIDRVRHACM
TGGCQAYWVCTLIEESELLEAQAAEATWEELKLALPELNVGLVHGRMKPAEKQAVMASFKQGELHLLVATTVIEVGVDVP
NASLMIIENPERLGLAQLHQLRGRVGRGAVASHCVLLYKTPLSKTAQIRLQVLRDSNDGFVIAQKDLEIRGPGELLGTRQ
TGNAEFKVADLLRDQAMIPEVQRLARHIHERYPQQAKALIERWMPETERYSNA

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=742232 QDW53_RS00375 WP_001560130.1 84478..86559(-) (recG) [Escherichia coli strain ETEC4069]
ATGAAAGGTCGCCTGTTAGATGCTGTCCCACTCAGTTCCCTAACGGGCGTTGGCGCAGCACTTAGTAACAAACTGGCGAA
AATCAACCTGCATACCGTGCAGGATCTACTCTTACACCTTCCCCTGCGCTACGAAGATCGCACCCATCTCTACCCCATCG
GAGAACTACTGCCGGGTGTTTATGCCACGGTGGAAGGCGAAGTGCTGAACTGCAATATCTCCTTCGGCGGTCGGCGGATG
ATGACCTGCCAGATCAGCGACGGTTCCGGCATTCTCACCATGCGCTTTTTCAATTTCAGCGCGGCAATGAAAAATAGCCT
GGCGACGGGCCGCCGTGTACTGGCTTATGGCGAAGCAAAGCGCGGTAAATATGGTGCGGAGATGATCCACCCGGAATACC
GCGTGCAGGGCGATCTCAGCACGCCAGAATTACAGGAAACGCTCACGCCGGTTTACCCAACTACGGAAGGCGTAAAGCAG
GCCACGCTGCGTAAATTAACCGATCAGGCGCTGGATCTGCTCGACACCTGCGCCATTGAAGAACTCCTGCCGCCGGAACT
GTCACAAGGAATGATGACGCTACCGGAAGCGTTGCGCACTTTGCACCGCCCGCCGCCGACGCTACAGCTTAGCGATCTCG
AAACCGGGCAGCATCCAGCACAACGCCGTCTGATTCTGGAAGAACTGCTTGCGCACAACCTCAGCATGTTGGCGTTACGC
GCTGGTGCACAACGTTTCCATGCCCAGCCGCTTAGCGCCAATGACGCGCTGAAAAATAAACTCCTCGCAGCCTTACCGTT
CAAGCCAACGGGCGCACAGGCACGCGTGGTGGCGGAGATCGAGCGCGATATGGCGCTGGATGCGCCGATGATGCGCCTGG
TACAGGGCGATGTAGGTTCCGGTAAAACGCTGGTCGCTGCCCTCGCCGCGCTGCGTGCGATTGCCCACGGCAAACAGGTG
GCATTGATGGCACCAACCGAATTACTCGCCGAACAGCACGCCAATAACTTCCGTAACTGGTTTGCACCGCTCGGTATCGA
AGTGGGCTGGCTCGCCGGTAAGCAGAAAGGTAAAGCGCGGCTGGCACAGCAGGAAGCCATCGCCAGCGGTCAGGTGCAGA
TGATTGTCGGAACACACGCCATCTTCCAGGAACAGGTGCAGTTTAACGGCCTGGCGCTGGTGATTATCGACGAACAGCAT
CGCTTTGGCGTGCATCAGCGTCTGGCATTGTGGGAGAAAGGCCAGCAGCAAGGCTTCCATCCGCATCAGTTGATCATGAC
CGCCACGCCGATCCCCCGCACGCTGGCGATGACTGCGTATGCTGATCTCGACACCTCGGTCATTGATGAACTGCCGCCAG
GCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACTCGCCGTACCGACATCATTGACCGCGTGCGCCACGCCTGCATG
ACTGGAGGTTGTCAGGCGTACTGGGTTTGTACGTTGATTGAAGAGTCAGAGTTACTGGAAGCGCAGGCGGCGGAAGCTAC
CTGGGAAGAGTTGAAACTGGCGTTGCCGGAACTGAACGTCGGCCTGGTACACGGGCGGATGAAACCTGCCGAGAAACAGG
CGGTGATGGCGTCGTTTAAACAAGGTGAACTACACCTGCTGGTTGCCACTACCGTTATTGAAGTCGGTGTTGATGTGCCT
AACGCCAGTCTGATGATTATCGAAAACCCTGAGCGTCTGGGTCTGGCGCAGTTACACCAGCTTCGCGGGCGTGTAGGTCG
TGGTGCGGTGGCTTCTCACTGCGTGCTGCTCTACAAAACGCCGCTCTCCAAAACCGCGCAAATTCGGCTGCAAGTGCTGC
GCGACAGCAACGACGGTTTTGTGATTGCACAGAAAGACTTAGAGATTCGCGGACCGGGTGAACTGTTAGGTACGCGTCAG
ACGGGTAATGCTGAGTTTAAAGTGGCGGATTTACTGCGCGACCAGGCGATGATCCCGGAAGTTCAGCGCCTGGCGCGACA
TATTCACGAACGTTACCCACAACAGGCAAAAGCCCTGATAGAACGCTGGATGCCAGAAACAGAACGCTACTCCAACGCAT
GA

Domains

Predicted by InterProScan.

DEAD/DEAH box helicase (DEAD)

(273-433)

RecG wedge domain (RecG_wedge)

(15-171)

Helicase conserved C-terminal domain (Helicase_C)

(510-588)

ATP-dependent DNA helicase RecG, domain 3, C-terminal (RecG_dom3_C)

(617-676)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  recG Neisseria meningitidis strain C311

52.232

96.97

0.506
  recG/mmsA Streptococcus pneumoniae R6

38.929

99.711

0.388
  recG/mmsA Streptococcus pneumoniae R36A

38.929

99.711

0.388
  recG Bacillus subtilis subsp. subtilis str. 168

39.058

94.949

0.371