Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   VroAM7_RS13415 Genome accession   NZ_AP019798
Coordinates   2880180..2881865 (+) Length   561 a.a.
NCBI ID   WP_143692959.1    Uniprot ID   A0A510I820
Organism   Vibrio rotiferianus strain AM7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2875180..2886865
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VroAM7_RS13395 (VroAM7_25240) pdhR 2876796..2877563 (-) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VroAM7_RS13400 (VroAM7_25250) ampD 2877968..2878519 (-) 552 WP_038885735.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VroAM7_RS13405 (VroAM7_25260) nadC 2878612..2879499 (+) 888 WP_088880393.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VroAM7_RS13410 (VroAM7_25270) pilA 2879727..2880176 (+) 450 WP_088880392.1 pilin Machinery gene
  VroAM7_RS13415 (VroAM7_25280) pilB 2880180..2881865 (+) 1686 WP_143692959.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VroAM7_RS13420 (VroAM7_25290) pilC 2881879..2883102 (+) 1224 WP_143692960.1 type II secretion system F family protein Machinery gene
  VroAM7_RS13425 (VroAM7_25300) pilD 2883173..2884042 (+) 870 WP_143692961.1 prepilin peptidase Machinery gene
  VroAM7_RS13430 (VroAM7_25310) coaE 2884043..2884657 (+) 615 WP_143692962.1 dephospho-CoA kinase -
  VroAM7_RS13435 (VroAM7_25320) zapD 2884686..2885426 (+) 741 WP_143692963.1 cell division protein ZapD -
  VroAM7_RS13440 (VroAM7_25330) yacG 2885490..2885684 (+) 195 WP_010450195.1 DNA gyrase inhibitor YacG -
  VroAM7_RS13445 (VroAM7_25340) rplS 2886260..2886613 (-) 354 WP_005379971.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 561 a.a.        Molecular weight: 62139.18 Da        Isoelectric Point: 5.8327

>NTDB_id=74128 VroAM7_RS13415 WP_143692959.1 2880180..2881865(+) (pilB) [Vibrio rotiferianus strain AM7]
MQASLVSALSKAGLLTPAQQASIAKETESLGIELPEALTRLKLFSPQELSETLANIFGLRVVSIDNYDYHSICSQLNQRE
LITLHNALPLKRSNSTLLLAIADPTNLQVEEDFRFATGLQIELVLVDWHILQSSIRRVYGKALSQEHASPRDINQDELIN
LVEVDDNELDTIEDLSQDSSPVSRYINQILLDAVRKGASDIHFEPYESIYRVRLRCDGILMEVQQPPSHLSRRLSARLKI
LSKLDIAERRLPQDGRIKLKLNNDTAIDMRVSTLPTLFGEKIVLRLLDNSSASLAIDHLGYSEAQKQAYLQALSRPQGMI
LMTGPTGSGKTVSLYAGLNILNQPEVNISTAEDPIEINLLGINQVQIQPKIGFTFAEALRSFLRQDPDIVMVGEIRDIET
AEIAMKASQTGHLVLSTLHTNSAAETVTRLSNMGITNYNLSSSLSLVIAQRLARKLCNHCKKITNSSNQQLLDIGITDEV
IYEANTRGCSECNNGYFGRTGIYEVMIFDEPLIQAVANGISSSQLEKLAVKNGMHTLKMSGIAKLKQGITSLNELQRVLF
L

Nucleotide


Download         Length: 1686 bp        

>NTDB_id=74128 VroAM7_RS13415 WP_143692959.1 2880180..2881865(+) (pilB) [Vibrio rotiferianus strain AM7]
ATGCAAGCAAGTTTAGTTAGCGCTCTTAGCAAGGCCGGATTATTGACTCCAGCGCAGCAGGCGTCTATAGCTAAAGAAAC
AGAGTCCTTAGGCATTGAGTTGCCTGAGGCTCTTACTCGTTTGAAGCTATTTTCACCGCAAGAACTGAGTGAAACATTAG
CCAATATATTTGGGCTAAGGGTCGTCAGTATAGATAATTATGACTACCACTCAATATGTTCACAGTTAAACCAGCGGGAA
CTCATTACTCTCCATAATGCACTGCCTTTAAAGCGCTCAAACTCAACACTTCTGTTAGCCATCGCAGACCCAACGAATTT
ACAAGTAGAGGAAGATTTCCGATTTGCCACGGGGTTGCAAATCGAACTTGTTTTAGTGGACTGGCACATATTGCAATCAT
CGATTAGAAGAGTATATGGCAAAGCCCTTTCTCAAGAGCATGCAAGTCCGAGAGATATCAACCAAGACGAACTCATCAAC
CTCGTTGAAGTGGATGACAATGAACTAGATACGATTGAAGATTTAAGCCAAGATAGCTCACCTGTCAGTCGTTATATCAA
CCAAATCCTTCTAGATGCTGTACGGAAAGGGGCGTCGGACATTCACTTTGAGCCTTATGAAAGCATTTACCGAGTGAGGC
TTCGATGTGATGGTATTCTCATGGAAGTACAACAACCTCCAAGTCACCTTAGTCGTCGCTTATCCGCTCGACTAAAAATC
CTATCCAAGCTCGATATTGCTGAAAGACGCTTACCTCAAGATGGTCGAATTAAGCTAAAACTTAACAACGATACCGCTAT
TGATATGCGAGTTTCGACACTTCCGACCTTATTCGGAGAGAAGATCGTTTTAAGGTTACTCGATAACAGTTCGGCATCAT
TAGCCATTGACCATCTAGGCTACAGCGAGGCACAGAAACAGGCATACCTACAAGCTCTCAGCCGCCCCCAGGGCATGATA
TTGATGACGGGACCAACCGGAAGTGGCAAAACCGTCTCTCTTTATGCTGGCTTAAACATTTTGAATCAACCAGAAGTGAA
TATATCTACCGCTGAAGATCCTATCGAAATCAACTTACTAGGTATCAACCAAGTTCAAATCCAGCCAAAAATAGGATTCA
CATTTGCAGAAGCCCTACGCTCATTTTTAAGACAAGACCCTGACATCGTTATGGTTGGAGAAATTCGAGATATTGAGACC
GCAGAAATCGCAATGAAGGCCTCTCAAACTGGTCATTTAGTGCTGTCCACCTTGCATACCAACTCTGCCGCAGAAACTGT
TACTCGCTTGAGCAATATGGGCATAACCAACTATAACTTGTCTTCTTCACTGAGCTTAGTGATTGCTCAGAGGTTAGCTC
GTAAGCTCTGCAATCATTGCAAAAAAATCACAAATAGCTCAAACCAACAACTGCTAGATATAGGAATTACGGATGAAGTA
ATTTATGAAGCAAATACAAGAGGTTGTAGCGAGTGTAACAATGGATATTTTGGCAGAACTGGCATTTATGAAGTTATGAT
CTTTGATGAACCACTCATTCAAGCGGTCGCTAACGGAATAAGTAGCTCCCAACTCGAGAAACTCGCAGTAAAAAATGGAA
TGCACACGCTAAAAATGTCAGGCATAGCAAAGTTGAAGCAAGGCATAACCAGCCTAAACGAACTACAGCGAGTTCTATTT
TTGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-143)

Type II/IV secretion system protein (T2SSE)

(188-455)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510I820

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

71.352

100

0.715
  pilB Vibrio campbellii strain DS40M4

70.285

100

0.704
  pilB Vibrio cholerae strain A1552

65.248

100

0.656
  pilB Acinetobacter baumannii D1279779

50.766

93.048

0.472
  pilB Legionella pneumophila strain ERS1305867

48.976

95.722

0.469
  pilB Acinetobacter baylyi ADP1

48.375

93.226

0.451
  pilF Neisseria gonorrhoeae MS11

43.54

100

0.439
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.388

99.643

0.373

Multiple sequence alignment