Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   VroAM7_RS13410 Genome accession   NZ_AP019798
Coordinates   2879727..2880176 (+) Length   149 a.a.
NCBI ID   WP_088880392.1    Uniprot ID   A0A510I815
Organism   Vibrio rotiferianus strain AM7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2874727..2885176
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  VroAM7_RS13395 (VroAM7_25240) pdhR 2876796..2877563 (-) 768 WP_005446261.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  VroAM7_RS13400 (VroAM7_25250) ampD 2877968..2878519 (-) 552 WP_038885735.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  VroAM7_RS13405 (VroAM7_25260) nadC 2878612..2879499 (+) 888 WP_088880393.1 carboxylating nicotinate-nucleotide diphosphorylase -
  VroAM7_RS13410 (VroAM7_25270) pilA 2879727..2880176 (+) 450 WP_088880392.1 pilin Machinery gene
  VroAM7_RS13415 (VroAM7_25280) pilB 2880180..2881865 (+) 1686 WP_143692959.1 type IV-A pilus assembly ATPase PilB Machinery gene
  VroAM7_RS13420 (VroAM7_25290) pilC 2881879..2883102 (+) 1224 WP_143692960.1 type II secretion system F family protein Machinery gene
  VroAM7_RS13425 (VroAM7_25300) pilD 2883173..2884042 (+) 870 WP_143692961.1 prepilin peptidase Machinery gene
  VroAM7_RS13430 (VroAM7_25310) coaE 2884043..2884657 (+) 615 WP_143692962.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 149 a.a.        Molecular weight: 15596.10 Da        Isoelectric Point: 10.0439

>NTDB_id=74127 VroAM7_RS13410 WP_088880392.1 2879727..2880176(+) (pilA) [Vibrio rotiferianus strain AM7]
MNVTKRKIQKGFTLIELMIVVAVIGVLAAIAIPQYQNYVAKSELGSGLATITSIRTNVEDYVVTNGAFPDGTTAGQKPTD
LGVIQPGNGTIRFVKAKGQLLLTFASTGNSPDVNSAKIALVRASGGTWTCKSTLDAPLLPKSCTKDATL

Nucleotide


Download         Length: 450 bp        

>NTDB_id=74127 VroAM7_RS13410 WP_088880392.1 2879727..2880176(+) (pilA) [Vibrio rotiferianus strain AM7]
ATGAACGTAACCAAACGAAAAATACAGAAAGGCTTTACACTGATCGAATTAATGATTGTTGTGGCTGTCATTGGAGTACT
CGCCGCAATCGCAATTCCTCAATATCAAAATTATGTCGCCAAGTCCGAGCTAGGTTCTGGCTTAGCTACCATTACTTCGA
TCAGAACTAACGTTGAAGATTATGTTGTCACCAATGGGGCATTCCCAGATGGCACCACTGCAGGTCAAAAGCCGACTGAC
CTGGGTGTTATTCAACCAGGTAACGGTACAATTCGTTTCGTGAAAGCAAAAGGTCAACTATTGCTTACCTTTGCATCGAC
AGGAAACAGCCCTGACGTAAATAGCGCAAAAATTGCATTGGTTAGAGCTTCAGGCGGTACATGGACTTGTAAATCAACCT
TAGATGCTCCTCTACTGCCTAAGTCCTGCACAAAAGACGCTACGCTATAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(6-31)

Pilin (bacterial filament) (Pilin)

(40-144)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A510I815

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

54.248

100

0.557
  pilA Vibrio cholerae strain A1552

54.248

100

0.557
  pilA Vibrio cholerae C6706

54.248

100

0.557
  pilA Pseudomonas aeruginosa PAK

45.638

100

0.456
  pilA Acinetobacter baumannii strain A118

44.366

95.302

0.423
  pilA Acinetobacter nosocomialis M2

40.127

100

0.423
  pilA/pilAI Pseudomonas stutzeri DSM 10701

40.69

97.315

0.396
  pilA Vibrio parahaemolyticus RIMD 2210633

43.704

90.604

0.396
  pilA2 Legionella pneumophila str. Paris

41.135

94.631

0.389
  pilA2 Legionella pneumophila strain ERS1305867

40.426

94.631

0.383
  pilA Haemophilus influenzae 86-028NP

37.255

100

0.383
  comP Acinetobacter baylyi ADP1

39.041

97.987

0.383

Multiple sequence alignment