Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilS   Type   Regulator
Locus tag   OEG79_RS16540 Genome accession   NZ_CP107549
Coordinates   3451854..3453446 (+) Length   530 a.a.
NCBI ID   WP_264146045.1    Uniprot ID   -
Organism   Pseudomonas sp. Z8(2022)     
Function   regulate pilin expression (predicted from homology)   
Competence regulation

Genomic Context

Location: 3446854..3458446
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OEG79_RS16515 (OEG79_16515) - 3447683..3448285 (-) 603 WP_413247517.1 TetR/AcrR family transcriptional regulator -
  OEG79_RS16520 (OEG79_16520) pgeF 3448386..3449117 (-) 732 WP_264146041.1 peptidoglycan editing factor PgeF -
  OEG79_RS16525 (OEG79_16525) rluD 3449114..3450088 (-) 975 WP_264146042.1 23S rRNA pseudouridine(1911/1915/1917) synthase RluD -
  OEG79_RS16530 (OEG79_16530) - 3450442..3451434 (+) 993 WP_264146043.1 outer membrane protein assembly factor BamD -
  OEG79_RS16535 (OEG79_16535) - 3451628..3451864 (+) 237 WP_264146044.1 PP0621 family protein -
  OEG79_RS16540 (OEG79_16540) pilS 3451854..3453446 (+) 1593 WP_264146045.1 sensor histidine kinase Regulator
  OEG79_RS16545 (OEG79_16545) pilR 3453449..3454789 (+) 1341 WP_264146046.1 sigma-54-dependent transcriptional regulator Regulator
  OEG79_RS16550 (OEG79_16550) thiO 3454802..3455902 (-) 1101 WP_264146047.1 glycine oxidase ThiO -
  OEG79_RS16555 (OEG79_16555) - 3455986..3456489 (+) 504 WP_264146048.1 GspH/FimT family protein -
  OEG79_RS16560 (OEG79_16560) - 3456633..3457115 (+) 483 WP_264146049.1 GspH/FimT family pseudopilin -
  OEG79_RS16565 (OEG79_16565) pilV 3457124..3457681 (+) 558 WP_264146050.1 type IV pilus modification protein PilV Machinery gene

Sequence

Protein


Download         Length: 530 a.a.        Molecular weight: 59384.55 Da        Isoelectric Point: 7.9016

>NTDB_id=740586 OEG79_RS16540 WP_264146045.1 3451854..3453446(+) (pilS) [Pseudomonas sp. Z8(2022)]
MTADHSLQGETQGRRIFRLYHFYRLSIGLALVLLISSNLDEQLLDVVHIELFRTGSWVYLILNILIAVLVQRPKHLMQLF
SLALVDVIFLSALFYAAGGTPSGIGNLLVVAVAIANILLRGRIGLLIAAIAAMALIYLTFYLSLSRPAAAAQYVQAGALG
ALCFAGALFIQGITRRLRLSESLAEQRAADVANLEELNEQILQRMRTGILVLDTQHRVLLANQGATQLLGRSELTGKIID
PYCPELVKRLQQWLHNPTLRPDSLQAQVDGPMLQPSFISLQRGEEQHILVFLDDISQIAQQAQQLKLASLGRLTAGIAHE
IRNPLGAISHAAQLLQESEDLQGPDQRLAQIIQDHSKRMNLVIENVLQLSRRRQAEPQLLDLKYWLHRFASEFRSSAPSD
RQIHLETQSGSLQTRMDPHQLTQVLTNLVQNGLRYSAQKHRVGQVWLRLFRDEFSDLPVLEILDDGPGVPPEQEQHIFEP
FYTTENKGTGLGLYISRELCQSNQARLDYKPREGGGSCFRITFAHPRKLS

Nucleotide


Download         Length: 1593 bp        

>NTDB_id=740586 OEG79_RS16540 WP_264146045.1 3451854..3453446(+) (pilS) [Pseudomonas sp. Z8(2022)]
GTGACCGCTGATCACAGTTTGCAAGGCGAAACCCAGGGGCGACGCATTTTCAGGCTCTATCATTTCTATCGCCTGAGCAT
CGGTCTGGCGCTGGTACTGCTGATCTCCAGCAACCTTGATGAGCAGCTTCTGGATGTGGTGCATATCGAGCTGTTTCGTA
CCGGCAGCTGGGTCTACCTGATCCTCAACATCCTCATTGCCGTACTGGTGCAACGCCCCAAGCACCTGATGCAGCTATTC
AGCCTGGCGCTGGTGGATGTGATCTTTCTCTCCGCGCTGTTCTACGCAGCTGGCGGCACACCCAGCGGCATCGGCAACCT
GCTGGTGGTGGCGGTGGCCATCGCCAATATCCTGCTTCGTGGCCGCATCGGCCTGCTGATCGCCGCCATTGCCGCAATGG
CGCTGATCTACCTGACCTTCTATCTCAGCCTCAGCCGCCCCGCCGCTGCCGCCCAGTACGTGCAGGCCGGCGCCCTCGGC
GCCCTATGCTTCGCCGGTGCGCTATTCATTCAAGGCATCACCCGCCGCCTGCGCCTGAGTGAAAGCCTGGCCGAGCAACG
CGCGGCAGACGTCGCCAATCTGGAGGAGCTCAATGAACAGATCCTGCAGCGCATGCGTACCGGCATCCTGGTGCTCGACA
CGCAGCATCGTGTGCTGCTGGCCAACCAGGGCGCGACACAGCTGCTGGGCCGCAGCGAACTGACCGGCAAGATCATCGAC
CCGTACTGCCCCGAGCTGGTGAAGCGCCTGCAACAGTGGTTGCACAACCCGACCCTGCGCCCGGACAGCCTGCAGGCGCA
GGTCGACGGGCCTATGCTGCAGCCCAGCTTCATCTCCCTGCAGCGCGGAGAAGAACAGCACATCCTGGTTTTTCTCGACG
ACATTTCGCAGATCGCCCAGCAGGCGCAACAGCTGAAACTGGCCTCGCTCGGCCGCCTGACCGCAGGCATCGCCCACGAA
ATCCGCAACCCGCTCGGTGCAATCAGCCACGCCGCGCAGCTGTTGCAGGAATCCGAAGATCTGCAAGGCCCCGATCAGCG
CCTGGCGCAGATCATTCAGGATCACTCCAAGCGCATGAATCTGGTGATCGAGAACGTTCTGCAGCTCTCGCGCCGACGTC
AGGCCGAGCCACAATTGCTGGACCTGAAGTACTGGCTGCACCGCTTCGCCAGCGAATTTCGCAGCTCGGCGCCGAGCGAC
CGGCAAATCCATCTGGAAACCCAGAGTGGCAGCCTGCAAACACGCATGGACCCCCATCAGCTGACCCAGGTGCTGACCAA
CCTGGTACAGAACGGCTTGCGCTACAGCGCGCAGAAACACCGGGTCGGCCAGGTCTGGCTGCGTCTGTTCCGCGACGAGT
TCAGCGACCTGCCGGTGCTGGAGATTCTCGACGACGGCCCCGGCGTGCCGCCCGAGCAGGAGCAGCACATCTTCGAGCCC
TTCTACACCACTGAAAACAAGGGCACCGGACTCGGCCTGTATATCTCCCGCGAGCTATGCCAGAGCAACCAGGCCCGCCT
CGACTACAAACCTCGTGAAGGGGGCGGCAGCTGCTTCCGCATCACCTTCGCCCATCCGCGCAAACTGAGCTGA

Domains

Predicted by InterproScan.

His Kinase A (phospho-acceptor) domain (HisKA)

(309-373)

Histidine kinase-, DNA gyrase B-, and HSP90-like ATPase (HATPase_c)

(417-523)

PAS domain (PAS_8)

(199-246)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilS Pseudomonas aeruginosa PAK

66.792

100

0.668