Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA/pilA3   Type   Machinery gene
Locus tag   AA2Tth_RS06390 Genome accession   NZ_AP019792
Coordinates   1192547..1193248 (+) Length   233 a.a.
NCBI ID   WP_143585827.1    Uniprot ID   -
Organism   Thermus thermophilus strain AA2-20     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1187547..1198248
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AA2Tth_RS06360 (TthAA220_12460) ilvB 1187983..1189671 (-) 1689 WP_143585820.1 biosynthetic-type acetolactate synthase large subunit -
  AA2Tth_RS06370 (TthAA220_12470) - 1189827..1190807 (-) 981 WP_018461695.1 YpdA family putative bacillithiol disulfide reductase -
  AA2Tth_RS06375 (TthAA220_12480) pilA/pilA1 1190973..1191443 (+) 471 WP_143585821.1 GspH/FimT family protein Machinery gene
  AA2Tth_RS06380 (TthAA220_12490) - 1191499..1191972 (+) 474 WP_143585823.1 GspH/FimT family protein -
  AA2Tth_RS06385 (TthAA220_12500) pilA/pilA2 1191990..1192550 (+) 561 WP_242004637.1 type II secretion system protein Machinery gene
  AA2Tth_RS06390 (TthAA220_12510) pilA/pilA3 1192547..1193248 (+) 702 WP_143585827.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  AA2Tth_RS06395 (TthAA220_12520) comZ 1193259..1194935 (+) 1677 WP_143585829.1 pilus assembly PilX N-terminal domain-containing protein Machinery gene
  AA2Tth_RS06400 (TthAA220_12530) - 1195156..1195506 (+) 351 WP_143585831.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  AA2Tth_RS06410 (TthAA220_12540) - 1195755..1196135 (+) 381 WP_018461702.1 DUF1640 domain-containing protein -
  AA2Tth_RS06415 (TthAA220_12550) - 1196211..1196753 (+) 543 WP_143585835.1 Uma2 family endonuclease -
  AA2Tth_RS06420 (TthAA220_12560) - 1196775..1197539 (-) 765 WP_242004638.1 IS982 family transposase -

Sequence

Protein


Download         Length: 233 a.a.        Molecular weight: 24935.35 Da        Isoelectric Point: 7.4615

>NTDB_id=74048 AA2Tth_RS06390 WP_143585827.1 1192547..1193248(+) (pilA/pilA3) [Thermus thermophilus strain AA2-20]
MKRGFTLVEVLVAMAILVVVLAVGVRYFASTSELARNTQARSELQDRVRMVMQVVTADLQMAGARYWNSGDQNQAFSLPL
PPLSGSDGGPKDTLTLYYVTSLRDLASACRRVDYGFEGDTLRRSDVSATPTSGSDCTTPSPNYQPLAEGILALDIQYQCS
DSTTRNTPDCGANAYPRSAKVTVAGYSLTPVTNPGPASLTTVTGKTLACPQGRACYALTQEVLMPNLKPLPTP

Nucleotide


Download         Length: 702 bp        

>NTDB_id=74048 AA2Tth_RS06390 WP_143585827.1 1192547..1193248(+) (pilA/pilA3) [Thermus thermophilus strain AA2-20]
ATGAAGCGCGGCTTCACCCTGGTGGAGGTCCTGGTGGCCATGGCCATCTTGGTGGTGGTCCTGGCCGTGGGCGTGCGCTA
CTTCGCAAGCACAAGCGAACTCGCCCGGAACACCCAGGCAAGGAGCGAGCTCCAGGACCGGGTGCGCATGGTGATGCAGG
TGGTGACCGCCGACCTGCAGATGGCGGGGGCCCGCTACTGGAATAGTGGCGACCAAAACCAAGCCTTCAGCCTCCCCCTT
CCCCCGCTTTCGGGAAGCGACGGAGGCCCTAAGGACACCCTAACCCTCTACTACGTCACGAGCCTCCGGGACCTGGCCTC
CGCCTGCCGCCGGGTGGACTACGGGTTTGAAGGGGACACCTTGCGTCGGAGCGACGTGAGCGCCACACCCACCTCGGGGA
GCGACTGCACCACCCCTTCCCCCAACTACCAACCCCTGGCCGAGGGCATCCTGGCCCTGGACATCCAATACCAGTGCAGC
GACAGCACCACCCGAAACACTCCGGACTGCGGCGCAAACGCCTACCCCCGCTCGGCCAAGGTTACGGTGGCGGGCTACTC
CCTGACCCCGGTGACCAACCCGGGCCCCGCCTCCCTCACCACCGTCACCGGAAAGACCCTCGCCTGCCCCCAGGGCCGGG
CCTGCTACGCCCTCACCCAGGAGGTCCTCATGCCCAACCTCAAGCCCCTGCCCACCCCCTAG

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(2-23)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA/pilA3 Thermus thermophilus HB27

93.133

100

0.931

Multiple sequence alignment