Detailed information NTG0074000

Bioinformatically predicted

Overview

• Name: pilU
• Type: Machinery gene
• Locus tag: K5658_RS02590
• Genome accession: NZ_AP019783
• Coordinates: 592086..593369 (+)
• Length: 427 a.a.
• NCBI ID: WP_221065436.1
• Uniprot ID: -
• Organism: Methylomagnum ishizawai strain RS11D-Pr
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 587086..598369

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K5658_RS02565 (MishRS11D_05100)	mazG	587435..588232 (+)	798	WP_221065431.1	nucleoside triphosphate pyrophosphohydrolase	-
K5658_RS02570 (MishRS11D_05110)	-	588354..589175 (-)	822	WP_221065432.1	PEP-CTERM sorting domain-containing protein	-
K5658_RS02575 (MishRS11D_05120)	nfi	589273..589935 (+)	663	WP_221065433.1	deoxyribonuclease V	-
K5658_RS02580 (MishRS11D_05130)	-	589972..590193 (-)	222	WP_221065434.1	acetyltransferase	-
K5658_RS02585 (MishRS11D_05140)	-	590485..591951 (+)	1467	WP_221065435.1	Ni/Fe hydrogenase subunit alpha	-
K5658_RS02590 (MishRS11D_05150)	pilU	592086..593369 (+)	1284	WP_221065436.1	PilT/PilU family type 4a pilus ATPase	Machinery gene
K5658_RS02595 (MishRS11D_05160)	-	593454..593951 (+)	498	WP_221065437.1	hydrogenase maturation protease	-
K5658_RS02600 (MishRS11D_05170)	-	594080..596206 (+)	2127	WP_221065438.1	SpoIIE family protein phosphatase	-
K5658_RS02605 (MishRS11D_05180)	-	596163..597686 (+)	1524	WP_221065439.1	PhnD/SsuA/transferrin family substrate-binding protein	-

Sequence

Protein

• Length: 427 a.a.
• Molecular weight: 47297.14 Da
• Isoelectric Point: 6.2023

>NTDB_id=74000 K5658_RS02590 WP_221065436.1 592086..593369(+) (pilU) [Methylomagnum ishizawai strain RS11D-Pr]
MKFQAYLKLLVYKDGSDLYLTANAPPAAKFQGKLTPIDNLMMTDESIREIAHSIMDAEQRREFEHRPEMNLAITEPGVGR
FRVNIFKQRNAYAMVVRNIKVKIPSLEELGLPETLKSVIMEKRGLVLFVGGTGSGKSTSLAALIDYRNTHSAGHIITIED
PIEFIHPHKKSLVNQREVGVDTQSYEDALKNTLRQAPDVILIGEIRSQDTMEYALAFAETGHLCLSTLHANNANQALDRI
INFFPEERRNQLLMDLSLNLRAFISQRLVPTVDGKRAAAIEILLGTPLVSDLIRKGEVHTIREVMEKSEGVGMQTFDNHL
VRMYRQGLIGEETAIKNADSANNVRLKINLGQPRMVEPEEEAPPEAAPAPGNKAETPPPERPKPAPGRSGLSLEPIASEE
ETPAAAAALDEPSFEWKPAKPLNRPGG

Nucleotide

• Length: 1284 bp

>NTDB_id=74000 K5658_RS02590 WP_221065436.1 592086..593369(+) (pilU) [Methylomagnum ishizawai strain RS11D-Pr]
ATGAAATTCCAAGCCTATCTCAAGCTCCTGGTCTACAAGGATGGTTCCGATCTTTACCTGACCGCCAACGCCCCGCCCGC
CGCCAAGTTCCAGGGCAAGCTCACGCCCATCGACAATCTGATGATGACCGACGAGAGCATCCGCGAAATCGCCCATTCGA
TCATGGACGCCGAGCAGCGCCGCGAATTCGAGCATCGGCCCGAGATGAACCTCGCCATCACCGAGCCGGGCGTGGGGCGG
TTCCGGGTCAATATCTTCAAGCAGCGCAACGCCTATGCCATGGTGGTGCGCAACATCAAGGTCAAGATTCCCAGCCTGGA
GGAATTGGGCCTCCCCGAGACCCTCAAGAGCGTCATCATGGAGAAGCGCGGGCTGGTGCTGTTCGTCGGCGGCACGGGGT
CGGGCAAATCGACCTCGCTGGCGGCCTTGATCGATTACCGCAACACCCATAGCGCCGGCCATATCATCACCATCGAAGAC
CCCATCGAGTTCATCCATCCGCACAAGAAGAGCCTGGTGAACCAGCGCGAGGTGGGGGTCGATACCCAGTCCTACGAGGA
CGCGCTCAAGAACACCCTCAGGCAAGCCCCGGACGTGATCCTGATCGGCGAAATCCGCTCCCAGGACACCATGGAATACG
CCCTGGCCTTCGCCGAGACCGGGCATCTCTGCCTCTCGACCCTGCACGCCAACAACGCCAACCAAGCCCTGGACCGCATC
ATCAATTTCTTCCCCGAAGAGCGGCGCAACCAGTTGTTGATGGATTTGTCGCTGAACCTCAGGGCCTTCATTTCCCAGCG
CCTGGTGCCGACGGTGGACGGCAAGCGCGCCGCCGCCATCGAAATCCTGCTGGGCACGCCCTTGGTGTCCGATTTGATCC
GCAAGGGCGAGGTCCATACCATCCGCGAGGTGATGGAGAAGTCCGAGGGCGTGGGGATGCAGACCTTCGACAACCATCTG
GTCCGCATGTACCGGCAGGGCTTGATCGGCGAGGAAACCGCGATCAAGAACGCCGATTCGGCCAACAATGTGCGGCTCAA
GATCAACCTGGGCCAACCGCGGATGGTCGAGCCGGAGGAGGAAGCCCCGCCCGAGGCCGCGCCCGCGCCGGGCAACAAGG
CCGAAACCCCGCCGCCCGAAAGACCCAAGCCCGCGCCGGGCCGTTCGGGCCTGAGTTTGGAACCCATCGCCAGCGAGGAG
GAAACGCCCGCCGCCGCCGCCGCCCTCGACGAGCCCAGTTTTGAATGGAAGCCCGCCAAGCCCCTCAACCGGCCCGGCGG
TTAG

Domains

Predicted by InterproScan.

• Type II/IV secretion system protein (T2SSE) (42-270)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilU	Pseudomonas stutzeri DSM 10701	60.511	82.436	0.499
pilU	Acinetobacter baylyi ADP1	54.237	82.904	0.45
pilU	Vibrio cholerae strain A1552	50.716	81.733	0.415