Detailed information NTG0073954

Bioinformatically predicted

Overview

• Name: pilR
• Type: Regulator
• Locus tag: MKFW12EY_RS18440
• Genome accession: NZ_AP019777
• Coordinates: 4094948..4096288 (-)
• Length: 446 a.a.
• NCBI ID: WP_054763602.1
• Uniprot ID: A0A172UC47
• Organism: Methylomonas koyamae strain Fw12E-Y
• Function: regulate pilin expression (predicted from homology) Competence regulation

Genomic Context

Location: 4089948..4101288

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
MKFW12EY_RS18425 (MKFW12EY_36680)	-	4091408..4092259 (-)	852	WP_054762725.1	HDOD domain-containing protein	-
MKFW12EY_RS18430 (MKFW12EY_36690)	-	4092440..4094275 (+)	1836	WP_054762703.1	EAL domain-containing protein	-
MKFW12EY_RS18435 (MKFW12EY_36700)	-	4094427..4094903 (+)	477	WP_221053533.1	hypothetical protein	-
MKFW12EY_RS18440 (MKFW12EY_36710)	pilR	4094948..4096288 (-)	1341	WP_054763602.1	sigma-54 dependent transcriptional regulator	Regulator
MKFW12EY_RS18445 (MKFW12EY_36720)	-	4096285..4097826 (-)	1542	WP_082881000.1	ATP-binding protein	-
MKFW12EY_RS18450 (MKFW12EY_36730)	sucC	4098006..4099175 (+)	1170	WP_054763603.1	ADP-forming succinate--CoA ligase subunit beta	-
MKFW12EY_RS18455 (MKFW12EY_36740)	sucD	4099175..4100047 (+)	873	WP_221053534.1	succinate--CoA ligase subunit alpha	-

Sequence

Protein

• Length: 446 a.a.
• Molecular weight: 49534.08 Da
• Isoelectric Point: 6.2593

>NTDB_id=73954 MKFW12EY_RS18440 WP_054763602.1 4094948..4096288(-) (pilR) [Methylomonas koyamae strain Fw12E-Y]
MNMPVTLVVDDEPDIRELLEITLNRMQIQTRSAATLAEAKHCLANQSFDLCLTDMRLPDGDGLELVDYIQAQGLTLPVAV
ITAHGSMDIAIKAMKKGAFDFLSKPVDLAVLRQLVSHALQISLNRVAEKERRTRDLLLGESAPMRNIRSKIDKVARNQAP
VYISGESGSGKELVARLIHQQSPRSEKAFIAINCGAIPHELMESEFFGHKKGSFTGAVSDKQGLFQAADGGTLFLDEVAD
LPLALQVKLLRAIQEKKIRPIGEQREIPVDVRLLSATHRDLAKMVQEGSFRQDLYYRINVIELNLPPLRARAADIPQLTE
HLLQGLARANGIAIPRLSDAALNALKQYYFPGNVRELENILERALALHEGGVIEVDDLNLPTGLDLAAAEDFDAEKMSLE
SYLEDIEKKALSSALEENRWNKTATAKYLGMSFRSLRYRLKKLGLE

Nucleotide

• Length: 1341 bp

>NTDB_id=73954 MKFW12EY_RS18440 WP_054763602.1 4094948..4096288(-) (pilR) [Methylomonas koyamae strain Fw12E-Y]
ATGAATATGCCTGTTACGTTAGTGGTCGACGACGAGCCCGATATTCGGGAACTGTTGGAAATCACGTTAAACCGGATGCA
GATCCAGACCCGTAGCGCGGCTACGCTCGCCGAAGCCAAGCATTGTCTGGCTAATCAATCCTTCGATTTATGTTTGACCG
ACATGCGCTTGCCGGACGGAGACGGCTTGGAACTGGTCGATTACATCCAGGCCCAAGGTTTGACGCTGCCGGTGGCGGTG
ATCACTGCCCACGGCAGCATGGATATCGCGATCAAGGCCATGAAAAAAGGCGCTTTCGATTTTCTGTCCAAGCCGGTCGA
TCTGGCGGTGTTAAGGCAATTGGTCAGCCATGCCTTGCAGATCTCGCTGAACCGGGTGGCCGAAAAGGAACGCCGCACCC
GCGATTTGTTGCTCGGGGAATCGGCGCCGATGCGCAATATCCGCTCCAAGATCGATAAAGTGGCGCGCAACCAGGCGCCG
GTTTACATCAGCGGCGAGTCGGGTTCCGGCAAGGAATTGGTGGCGCGGCTGATTCACCAGCAAAGCCCGCGCAGCGAAAA
GGCTTTTATCGCCATCAATTGCGGCGCGATTCCGCACGAATTGATGGAAAGCGAATTTTTCGGTCACAAAAAAGGCAGCT
TCACCGGGGCGGTCAGCGATAAGCAAGGTTTGTTTCAAGCCGCGGACGGCGGCACGCTGTTTCTGGACGAAGTGGCCGAT
TTGCCGTTGGCTTTGCAAGTCAAATTACTGCGGGCGATACAGGAAAAGAAAATCCGTCCGATCGGCGAGCAGCGCGAAAT
TCCGGTGGACGTGCGTTTGCTGAGCGCGACCCACAGGGATTTGGCAAAAATGGTTCAGGAAGGCAGTTTTCGGCAGGACT
TGTATTACCGGATCAACGTCATCGAACTGAATCTGCCGCCATTACGGGCGCGAGCCGCCGATATTCCGCAATTGACCGAA
CATCTGCTGCAAGGTTTGGCGCGGGCCAACGGCATTGCTATCCCGCGCCTGTCGGATGCGGCGTTGAATGCGCTGAAACA
GTATTATTTTCCGGGCAACGTCCGCGAGTTGGAAAATATTTTGGAGCGGGCTTTGGCGCTGCACGAGGGCGGCGTGATCG
AGGTCGACGATTTGAATTTGCCGACCGGTTTGGACTTGGCCGCGGCGGAAGATTTCGATGCCGAAAAAATGTCGCTGGAA
AGTTATCTGGAAGATATCGAGAAAAAAGCGCTGAGTTCGGCTTTGGAGGAAAACCGCTGGAATAAAACCGCGACGGCTAA
ATATTTGGGTATGAGTTTCCGCTCGTTGCGTTACCGGTTGAAGAAACTCGGTCTGGAATAA

Domains

Predicted by InterproScan.

• Bacterial regulatory protein, Fis family (HTH_8) (403-443)

• Sigma-54 interaction domain (Sigma54_activat) (138-303)

• Response regulator receiver domain (Response_reg) (7-115)

3D structure

• AlphaFold DB: A0A172UC47

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilR	Pseudomonas aeruginosa PAK	63.333	100	0.639
pilR	Acinetobacter baumannii strain A118	50.644	100	0.529
luxO	Vibrio cholerae strain A1552	39.189	99.552	0.39