Detailed information NTG0073585

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: K6J89_RS13700
• Genome accession: NZ_AP024621
• Coordinates: 2619813..2620430 (-)
• Length: 205 a.a.
• NCBI ID: WP_004398514.1
• Uniprot ID: P39694
• Organism: Bacillus subtilis strain BEST3095
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2614813..2625430

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K6J89_RS13670 (BsBEST3095_26140)	rpsT	2615115..2615381 (+)	267	WP_003229989.1	30S ribosomal protein S20	-
K6J89_RS13675 (BsBEST3095_26150)	holA	2615396..2616439 (-)	1044	WP_003229987.1	DNA polymerase III subunit delta	-
K6J89_RS13680	-	2616479..2616628 (-)	150	WP_003229985.1	hypothetical protein	-
K6J89_RS13685 (BsBEST3095_26160)	yqzM	2616669..2616803 (+)	135	WP_003229983.1	YqzM family protein	-
K6J89_RS13690	comEC	2616843..2619173 (-)	2331	WP_009967776.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
K6J89_RS13695 (BsBEST3095_26190)	comEB	2619177..2619746 (-)	570	WP_003229978.1	ComE operon protein 2	-
K6J89_RS13700 (BsBEST3095_26200)	comEA	2619813..2620430 (-)	618	WP_004398514.1	competence protein ComEA	Machinery gene
K6J89_RS13705 (BsBEST3095_26210)	comER	2620514..2621335 (+)	822	WP_004398597.1	late competence protein ComER	-
K6J89_RS13710 (BsBEST3095_26220)	yqeM	2621401..2622144 (-)	744	WP_003229973.1	class I SAM-dependent methyltransferase	-
K6J89_RS13715 (BsBEST3095_26230)	rsfS	2622141..2622497 (-)	357	WP_003229971.1	ribosome silencing factor	-
K6J89_RS13720 (BsBEST3095_26240)	yqeK	2622515..2623075 (-)	561	WP_004399059.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
K6J89_RS13725 (BsBEST3095_26250)	nadD	2623065..2623634 (-)	570	WP_004398676.1	nicotinate-nucleotide adenylyltransferase	-
K6J89_RS13730 (BsBEST3095_26260)	yhbY	2623646..2623936 (-)	291	WP_003226133.1	ribosome assembly RNA-binding protein YhbY	-
K6J89_RS13735 (BsBEST3095_26270)	aroE	2623930..2624772 (-)	843	WP_003229967.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 205 a.a.
• Molecular weight: 21769.45 Da
• Isoelectric Point: 4.7220

>NTDB_id=73585 K6J89_RS13700 WP_004398514.1 2619813..2620430(-) (comEA) [Bacillus subtilis strain BEST3095]
MNWLNQHKKAIILAASAAVFTAIMIFLATGKNKEPVKQAVPTETENTVVKQEANNDESNETIVIDIKGAVQHPGVYEMRT
GDRVSQAIEKAGGTSEQADEAQVNLAEILQDGTVVYIPKKGEETAVQQGGGGSVQSDGGKGALVNINTATLEELQGISGV
GPSKAEAIIAYREENGRFQTIEDITKVSGIGEKSFEKIKSSITVK

Nucleotide

• Length: 618 bp

>NTDB_id=73585 K6J89_RS13700 WP_004398514.1 2619813..2620430(-) (comEA) [Bacillus subtilis strain BEST3095]
ATGAATTGGTTGAATCAGCATAAGAAAGCAATTATTTTAGCGGCTTCTGCGGCTGTTTTCACAGCGATTATGATCTTTCT
GGCCACAGGGAAAAATAAAGAGCCGGTGAAGCAAGCTGTACCAACAGAGACAGAAAATACAGTGGTAAAGCAGGAAGCAA
ACAACGACGAGTCAAACGAAACAATTGTGATAGACATCAAAGGTGCTGTTCAGCATCCTGGCGTTTATGAAATGCGAACA
GGGGACAGAGTATCTCAGGCAATTGAGAAAGCGGGCGGGACCAGTGAACAAGCAGACGAAGCGCAAGTAAATTTGGCGGA
GATTCTGCAGGACGGGACAGTGGTGTACATCCCGAAAAAGGGAGAGGAAACAGCAGTGCAGCAAGGTGGCGGAGGGTCTG
TCCAAAGCGATGGAGGGAAGGGAGCGCTGGTGAATATCAATACAGCAACCTTAGAGGAGTTACAAGGCATCTCAGGGGTG
GGGCCATCCAAAGCTGAAGCTATTATTGCATACCGGGAGGAAAACGGTCGTTTCCAAACAATTGAAGATATCACTAAGGT
TTCAGGAATAGGTGAAAAGTCATTTGAGAAAATAAAGTCTTCCATTACAGTAAAGTGA

Domains

Predicted by InterProScan.

• Helix-hairpin-helix motif (HHH_3) (142-203)

• SLBB domain (SLBB) (63-118)

3D structure

• PDB: 8DFK

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	100	100	1
comEA	Staphylococcus aureus MW2	37.273	100	0.4
comEA	Staphylococcus aureus N315	36.364	100	0.39