Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   N8S79_RS09630 Genome accession   NZ_CP106678
Coordinates   2018107..2018967 (-) Length   286 a.a.
NCBI ID   WP_046850015.1    Uniprot ID   A0A0F7KGJ7
Organism   Nitrosomonas sp. PLL12-2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2013107..2023967
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8S79_RS09600 argJ 2013275..2014504 (+) 1230 WP_046850010.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  N8S79_RS09605 - 2014511..2015398 (+) 888 WP_046850011.1 ATP-binding protein -
  N8S79_RS09610 - 2015417..2016367 (+) 951 WP_046850012.1 Nudix family hydrolase -
  N8S79_RS09615 - 2016410..2016610 (-) 201 WP_082110395.1 DNA gyrase inhibitor YacG -
  N8S79_RS09620 zapD 2016623..2017378 (-) 756 WP_046850013.1 cell division protein ZapD -
  N8S79_RS09625 coaE 2017483..2018103 (-) 621 WP_046850014.1 dephospho-CoA kinase -
  N8S79_RS09630 pilD 2018107..2018967 (-) 861 WP_046850015.1 prepilin peptidase Machinery gene
  N8S79_RS09635 pilC 2019000..2020223 (-) 1224 WP_046850016.1 type II secretion system F family protein Machinery gene
  N8S79_RS09640 - 2020515..2021423 (-) 909 WP_046850017.1 SDR family oxidoreductase -
  N8S79_RS09645 - 2021468..2022511 (+) 1044 WP_046850018.1 CDP-6-deoxy-delta-3,4-glucoseen reductase -
  N8S79_RS09650 - 2022514..2023692 (-) 1179 WP_046850019.1 heme biosynthesis protein HemY -

Sequence

Protein


Download         Length: 286 a.a.        Molecular weight: 32027.88 Da        Isoelectric Point: 8.0540

>NTDB_id=734069 N8S79_RS09630 WP_046850015.1 2018107..2018967(-) (pilD) [Nitrosomonas sp. PLL12-2]
MTLTDSLQHSSAIFIAFSTIIGIIVGSFLNVVIYRLPRMMERAWHQQCAELRGENIHHSPVFNLVIPRSTCPQCEHRITV
LENIPLISYLFLKGYCSQCNTRISLRYPIVEGFTGIMSGFTAWHFGFGLTTIAALLFIWAMIALTFIDLESQLLPDTITL
PLLWIGLLFNLDNGFIEIRSAIIGTVAGYLVLWTIYWGFKLITGKEGMGYGDFKLLAAIGAWLGWQMLPLVILFSSFAGG
VISFILIITNKFQRNTTIPFGPYLAAGGLIALFWGNKINHAYFGLF

Nucleotide


Download         Length: 861 bp        

>NTDB_id=734069 N8S79_RS09630 WP_046850015.1 2018107..2018967(-) (pilD) [Nitrosomonas sp. PLL12-2]
ATGACATTAACCGATTCTCTTCAGCATTCATCGGCAATTTTTATCGCTTTTAGCACTATCATTGGAATAATAGTGGGTAG
TTTTTTGAATGTAGTCATTTACCGGCTTCCCAGAATGATGGAACGAGCATGGCACCAACAATGTGCTGAATTACGGGGAG
AGAATATTCATCATTCACCGGTTTTCAATCTGGTCATACCTCGCTCTACTTGCCCTCAATGTGAGCATAGAATTACCGTA
CTCGAAAATATTCCCCTCATCAGTTATTTATTCCTAAAAGGATACTGTTCACAATGCAATACGCGTATTTCCCTTCGCTA
CCCCATTGTCGAAGGATTTACAGGCATTATGAGCGGCTTTACGGCATGGCACTTCGGATTTGGCTTGACTACTATCGCCG
CCTTGTTGTTTATTTGGGCGATGATTGCCCTTACCTTTATCGATTTGGAGAGCCAATTATTACCCGATACGATTACATTA
CCTTTACTCTGGATCGGGCTTTTATTTAATCTGGATAATGGTTTTATTGAAATTCGATCAGCTATTATAGGCACAGTTGC
CGGTTATCTTGTTCTATGGACTATTTATTGGGGATTCAAACTGATTACGGGCAAAGAAGGAATGGGTTATGGTGATTTCA
AATTACTCGCCGCAATTGGTGCATGGCTAGGCTGGCAAATGTTACCATTAGTCATTCTATTTTCCTCATTTGCAGGAGGA
GTTATTAGTTTCATATTGATAATTACCAATAAATTTCAAAGAAATACTACCATTCCATTTGGCCCCTATCTTGCTGCCGG
AGGTTTGATTGCCTTATTCTGGGGGAATAAGATTAATCACGCTTACTTTGGTTTATTTTAG

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(20-125)

Type IV leader peptidase family (Peptidase_A24)

(136-242)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0F7KGJ7

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

52.364

96.154

0.504
  pilD Vibrio campbellii strain DS40M4

50

98.601

0.493
  pilD Acinetobacter nosocomialis M2

46.619

98.252

0.458
  pilD Acinetobacter baumannii D1279779

45.714

97.902

0.448
  pilD Neisseria gonorrhoeae MS11

45.802

91.608

0.42