Detailed information NTG0073363

Bioinformatically predicted

Overview

• Name: comGF
• Type: Machinery gene
• Locus tag: GOZ34_RS07550
• Genome accession: NZ_AP019712
• Coordinates: 1592585..1593082 (-)
• Length: 165 a.a.
• NCBI ID: WP_001825340.1
• Uniprot ID: A0A2S6DDC2
• Organism: Staphylococcus aureus strain Tokyo12480
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1587585..1598082

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
GOZ34_RS07525 (TMSFP480_14550)	gcvPB	1587747..1589219 (-)	1473	WP_000202179.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPB	-
GOZ34_RS07530 (TMSFP480_14560)	gcvPA	1589212..1590558 (-)	1347	WP_000019698.1	aminomethyl-transferring glycine dehydrogenase subunit GcvPA	-
GOZ34_RS07535 (TMSFP480_14570)	gcvT	1590578..1591669 (-)	1092	WP_155978173.1	glycine cleavage system aminomethyltransferase GcvT	-
GOZ34_RS07540 (TMSFP480_14580)	-	1591828..1592352 (-)	525	WP_001015120.1	shikimate kinase	-
GOZ34_RS07545	-	1592342..1592488 (-)	147	WP_001789879.1	hypothetical protein	-
GOZ34_RS07550 (TMSFP480_14590)	comGF	1592585..1593082 (-)	498	WP_001825340.1	competence type IV pilus minor pilin ComGF	Machinery gene
GOZ34_RS07555 (TMSFP480_14600)	comGE	1593000..1593299 (-)	300	WP_173020051.1	competence protein ComGE	Machinery gene
GOZ34_RS07560 (TMSFP480_14610)	comGD	1593286..1593732 (-)	447	WP_001796473.1	competence type IV pilus minor pilin ComGD	Machinery gene
GOZ34_RS07565 (TMSFP480_14620)	comGC	1593710..1594021 (-)	312	WP_000472256.1	competence type IV pilus major pilin ComGC	Machinery gene
GOZ34_RS07570 (TMSFP480_14630)	comGB	1594035..1595105 (-)	1071	WP_000776418.1	competence type IV pilus assembly protein ComGB	Machinery gene
GOZ34_RS07575 (TMSFP480_14640)	comGA	1595077..1596051 (-)	975	WP_000697220.1	competence type IV pilus ATPase ComGA	Machinery gene
GOZ34_RS07580 (TMSFP480_14650)	-	1596103..1596726 (-)	624	WP_001223008.1	MBL fold metallo-hydrolase	-
GOZ34_RS07585 (TMSFP480_14660)	-	1596723..1597052 (-)	330	WP_029549654.1	MTH1187 family thiamine-binding protein	-
GOZ34_RS07590 (TMSFP480_14670)	-	1597052..1598038 (-)	987	WP_000161315.1	ROK family glucokinase	-

Sequence

Protein

• Length: 165 a.a.
• Molecular weight: 19179.92 Da
• Isoelectric Point: 10.2192

>NTDB_id=73363 GOZ34_RS07550 WP_001825340.1 1592585..1593082(-) (comGF) [Staphylococcus aureus strain Tokyo12480]
MILSKVTNKFVLFQKIPLLIKRHVYSINVKAFSLIEMLVAMMVISITLLIVPDLIRLSKTFLIESRELTTVDFEFFSRDI
LEDFKGVDRNDIEIRQQRIILHKGEEMIEYKLINNKIIKVVNDRGNITLINNVTAFTANIYYKSIIKITITVKVGTNLQT
KTIYV

Nucleotide

• Length: 498 bp

>NTDB_id=73363 GOZ34_RS07550 WP_001825340.1 1592585..1593082(-) (comGF) [Staphylococcus aureus strain Tokyo12480]
ATGATATTAAGCAAAGTGACCAACAAATTTGTGCTATTTCAAAAAATACCACTTCTTATCAAAAGACATGTATACAGTAT
TAATGTCAAAGCTTTTTCGCTTATTGAAATGTTAGTAGCGATGATGGTTATAAGCATAACTTTACTAATTGTTCCAGACT
TAATTAGACTTAGCAAAACTTTTCTAATTGAAAGTAGGGAATTAACAACTGTAGATTTCGAATTTTTCTCAAGAGATATT
CTAGAGGATTTTAAAGGTGTAGATAGAAACGATATTGAAATTAGGCAACAGCGTATCATTTTACATAAAGGTGAAGAAAT
GATCGAATACAAATTAATAAATAATAAAATTATTAAAGTTGTAAATGACAGAGGAAATATAACATTGATTAATAATGTTA
CAGCATTTACTGCAAATATCTACTATAAATCTATTATTAAAATAACGATAACAGTTAAAGTCGGTACAAATCTGCAGACT
AAAACTATTTATGTATAA

Domains

Predicted by InterproScan.

• Prokaryotic N-terminal methylation motif (N_methyl) (28-51)

• Putative Competence protein ComGF (ComGF) (68-155)

3D structure

• AlphaFold DB: A0A2S6DDC2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comGF	Staphylococcus aureus N315	98.182	100	0.982
comGF	Staphylococcus aureus MW2	96.97	100	0.97